GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Three Characteristics Associated with Enterotoxigenic Escherichia coli Isolated from Man
    American Society for Microbiology ; 1973
    In:  Infection and Immunity Vol. 8, No. 3 ( 1973-09), p. 322-328
    Details
    In: Infection and Immunity, American Society for Microbiology, Vol. 8, No. 3 ( 1973-09), p. 322-328
    Abstract: In Dacca, Bangladesh, potent enterotoxin-producing Escherichia coli were isolated from many hospital cases of acute cholera-like diarrhea. Enterotoxigenic (tox + ) and non-enterotoxigenic (tox − ) isolates of E. coli were used to investigate possible means of differentiating tox + E. coli from those (tox − ) of the normal flora. The majority (81%) of the tox + E. coli studied were found to be negative for sucrose fermentation, 85% exhibited retarded growth in a peptone medium at pH 8.5, and 92% released large amounts of ammonium sulfate precipitable materials into culture supernatant fluids; 66.6% exhibited all three of these properties. For the tox − group the respective values were found to be 50%, 31%, and 34%; only 9.3% exhibited all three properties. These results indicate that it may be possible to use phenotypic characteristics other than antigenic composition and enterotoxin production for the identification of enterotoxigenic E. coli.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    URL: Article
    DOI: 10.1128/iai.8.3.322-328.1973
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1973
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Helicobacter pylori cagA Status and s and m Alleles of vacA in Isolates from Individuals with a Variety of H. pylori -Associated Gastric Diseases
    American Society for Microbiology ; 1998
    In:  Journal of Clinical Microbiology Vol. 36, No. 11 ( 1998-11), p. 3435-3437
    Details
    In: Journal of Clinical Microbiology, American Society for Microbiology, Vol. 36, No. 11 ( 1998-11), p. 3435-3437
    Abstract: The cagA gene was detected in 100% of 16 Helicobacter pylori isolates from patients with gastric carcinoma versus 78% of 18 isolates from patients with duodenal ulcers ( P = 0.344) and only 64% of 22 isolates from patients with gastritis only ( P = 0.005) in Brazil. Also, there was a significant association between isolation of cagA + s1-type vacA H. pylori in cases of stomach cancer and ulcers as opposed to cases of gastritis only ( P = 0.004), but this was not true in Houston ( P = 0.238), where 94% of all isolates were cagA + .
    Type of Medium: Online Resource
    ISSN: 0095-1137 , 1098-660X
    URL: Article
    DOI: 10.1128/JCM.36.11.3435-3437.1998
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1998
    detail.hit.zdb_id: 1498353-9
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Stimulation of Adenyl Cyclase by Escherichia coli Enterotoxin
    Springer Science and Business Media LLC ; 1972
    In:  Nature New Biology Vol. 236, No. 66 ( 1972-4), p. 137-138
    Details
    In: Nature New Biology, Springer Science and Business Media LLC, Vol. 236, No. 66 ( 1972-4), p. 137-138
    Type of Medium: Online Resource
    ISSN: 0090-0028 , 2058-1092
    URL: Article
    DOI: 10.1038/newbio236137a0
    RVK:
    TA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 1972
    detail.hit.zdb_id: 2590703-7
    SSG: 11
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Transfer of an ST:LT:CFA/II plasmid into Escherichia coli K-12 strain RR1 by contransformation with PSC301 plasmid DNA
    Oxford University Press (OUP) ; 1980
    In:  FEMS Microbiology Letters Vol. 8, No. 4 ( 1980-08), p. 251-254
    Details
    In: FEMS Microbiology Letters, Oxford University Press (OUP), Vol. 8, No. 4 ( 1980-08), p. 251-254
    Type of Medium: Online Resource
    ISSN: 0378-1097 , 1574-6968
    URL: Issue
    URL: Article
    DOI: 10.1111/fml.1980.8.issue-4
    DOI: 10.1111/j.1574-6968.1980.tb05089.x
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1980
    detail.hit.zdb_id: 1501716-3
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Immunoprotective oral whole cell vaccine for enterotoxigenic Escherichia coli diarrhea prepared by in situ destruction of chromosomal and plasmid DNA with colicin E2
    Oxford University Press (OUP) ; 1988
    In:  FEMS Microbiology Letters Vol. 47, No. 1 ( 1988-01), p. 9-18
    Details
    In: FEMS Microbiology Letters, Oxford University Press (OUP), Vol. 47, No. 1 ( 1988-01), p. 9-18
    Type of Medium: Online Resource
    ISSN: 0378-1097 , 1574-6968
    URL: Issue
    URL: Article
    DOI: 10.1111/fml.1988.47.issue-1
    DOI: 10.1111/j.1574-6968.1988.tb02485.x
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1988
    detail.hit.zdb_id: 1501716-3
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Helicobacter pylori Adhesins: Review and Perspectives
    Wiley ; 2000
    In:  Helicobacter Vol. 5, No. 4 ( 2000-12), p. 183-195
    Details
    In: Helicobacter, Wiley, Vol. 5, No. 4 ( 2000-12), p. 183-195
    Abstract: It is highly unlikely that chronic infection with H. pylori could occur in the absence of adhesin–host cell interactions. Also, there is no evidence that any of the serious outcomes of H. pylori infection such as gastric and duodenal ulcers, gastric cancer or mucosa‐associated lymphoid tissue (MALT) lymphoma could occur without prior colonization of the gastric epithelium mediated by H. pylori adhesins. H. pylori is highly adaptable, as evidenced by the fact that it can occupy a single host for decades. An important facet of this adaptability is its ability to physically interact with various types of host cells and also with host mucins and extracellular matrix proteins using a number of different adhesins displaying a variety of unique receptor specificities. Thus it is highly unlikely that any one particular H. pylori adhesin will ever be proven responsible for a particular outcome such as duodenal ulcer, MALT lymphoma, or adenocarcinoma. Also, while the search for additional H. pylori adhesins should and certainly will continue, we suggest that the scope of this effort should be expanded to include investigations into the patterns of expression and interaction between individual outer membrane proteins. Which of the numerous H. pylori outer membrane proteins (OMPs) actually function as adhesins (i.e., have receptor‐binding sites) and which OMPs are simply necessary for optimal display of the adhesive OMPs? There are many other important questions about H. pylori adhesins waiting to be answered. For example, which adhesins are responsible for loose adherence to host cells and which adhesins are responsible for intimate, or membrane‐to‐membrane, adherence, and do these adhesins normally work in concert or in a sequential fashion? Also, is a specific type of adhesin necessary for type IV protein translocation into host cells and, if so, is adhesin expression coregulated with the effector protein export?
    Type of Medium: Online Resource
    ISSN: 1083-4389 , 1523-5378
    URL: Article
    DOI: 10.1046/j.1523-5378.2000.00029.x
    Language: English
    Publisher: Wiley
    Publication Date: 2000
    detail.hit.zdb_id: 2020336-6
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    New Surface-Associated Heat-Labile Colonization Factor Antigen (CFA/II) Produced by Enterotoxigenic Escherichia coli of Serogroups O6 and O8
    American Society for Microbiology ; 1978
    In:  Infection and Immunity Vol. 21, No. 2 ( 1978-08), p. 638-647
    Details
    In: Infection and Immunity, American Society for Microbiology, Vol. 21, No. 2 ( 1978-08), p. 638-647
    Abstract: Enterotoxigenic Escherichia coli (ETEC) belonging to serogroups O6 and O8 do not possess the H-10407-type colonization factor antigen (CFA/I). However, these frequently isolated ETEC were found to possess a second and distinct heat-labile surface-associated colonization factor antigen, termed CFA/II. Whereas CFA/I mediates mannose-resistant hemagglutination of human group A erythrocytes, CFA/II does not. CFA/II mediates mannose-resistant hemagglutination of bovine erythrocytes, and mannose-resistant hemagglutination is rapid only at reduced temperature (4°C). Because CFA/II, like CFA/I, is spontaneously lost by many ETEC isolates in the laboratory, it was possible to produce specific anti-CFA/II serum by preparing antiserum against living cells of a prototype strain (PB-176) and adsorbing this serum with living and heat-treated cells of its CFA/II-negative derivative strain PB-176-P. This serum, which neutralized the colonization factor activity of CFA/II-positive strains in infant rabbits, was employed to confirm the presence of CFA/II on ETEC which exhibited mannose-resistant hemagglutination of bovine but not human erythrocytes. CFA/II, like CFA/I, mediates adherence of the bacteria to the mucosal surface of the small intestine, as demonstrated by indirect immunofluorescence. CFA/II appears to be an important virulence factor for humans since CFA/II-positive ETEC are frequently isolated from diarrhea cases, particularly travelers' diarrhea, in Mexico; these ETEC were not uncommon in a collection of isolates from Bangladesh. The O6:H16 strain of ETEC responsible for an outbreak of diarrhea in the United States was also shown to be CFA/II positive. CFA/I and CFA/II were never found on the same serotypes of ETEC, but 98% of the heat-stable and heat-labile enterotoxin-producing ETEC belonging to the frequently isolated serogroups O6, O8, O15, O25, O63, and O78 were positive for either CFA/I or CFA/II.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    URL: Article
    DOI: 10.1128/iai.21.2.638-647.1978
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1978
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Enzyme-Linked Immunosorbent Assay for Quantitating the Humoral Immune Response to the Colonization Factor Antigen of Enterotoxigenic Escherichia coli
    American Society for Microbiology ; 1980
    In:  Infection and Immunity Vol. 27, No. 2 ( 1980-02), p. 525-531
    Details
    In: Infection and Immunity, American Society for Microbiology, Vol. 27, No. 2 ( 1980-02), p. 525-531
    Abstract: The enzyme-linked immunosorbent assay technique was used to quantitate, in milligrams per milliliter, anti-colonization factor antigen/I (CFA/I) immunoglobulin G (IgG) in acute- and convalescent-phase sera of individuals who experienced diarrhea associated with CFA/I-positive enterotoxigenic Escherichia coli . Purified CFA/I was used as antigen to coat polystyrene Microtiter plate wells for the determination of anti-CFA/I antibody. A reference anti-CFA/I IgG preparation was obtained by affinity chromatography of a high-titered serum with a CFA/I-Sepharose 4B column; IgG was the only class of immunoglobulin detectable in this serum as anti-CFA/I. Goat anti-human IgG conjugated to alkaline phosphatase was used in the enzyme-linked immunosorbent assay. Quantitation of IgG in the reference anti-CFA/I serum was achieved by comparison with a known sample of pure human IgG. Anti-CFA/I in test sera was quantitated by titration with CFA/I-coated Microtiter plate wells in the enzyme-linked immunosorbent assay, using a standard curve obtained with the reference anti-CFA/I serum. Anti-CFA/I IgG in paired sera was determined as percentage of total IgG by using the radial immunodiffusion technique to quantitate total IgG for each test serum. Diarrhea with isolation of CFA/I-positive enterotoxigenic E. coli was associated with a significant rise in serum anti-CFA/I IgG when these values were expressed as either milligrams of IgG per milliliter or as percentage of total IgG, although the response varied quantitatively and nonresponders were detected. None of the matched controls showed an anti-CFA/I IgG response. Further elucidation of the immune response to enterotoxigenic E. coli can now be accomplished by applying these methods to determine the class and specificity of immunoglobulins in external secretions such as saliva and intestinal contents.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    URL: Article
    DOI: 10.1128/iai.27.2.525-531.1980
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1980
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Polymyxin B-Induced Release of Low-Molecular-Weight, Heat-Labile Enterotoxin from Escherichia coli
    American Society for Microbiology ; 1974
    In:  Infection and Immunity Vol. 10, No. 5 ( 1974-11), p. 1010-1017
    Details
    In: Infection and Immunity, American Society for Microbiology, Vol. 10, No. 5 ( 1974-11), p. 1010-1017
    Abstract: Polymyxin B-induced release of enterotoxin from Escherichia coli strain H-10407 was demonstrated. Incubation of E. coli cells derived from 6-h cultures with polymyxin caused the rapid release of enterotoxin with a molecular weight of approximately 20,000, as estimated by the gel filtration technique. The rapidity of the release of enterotoxin indicates that it probably resides in the periplasmic space of the cell. The low-molecular-weight enterotoxin possessed vascular permeability factor and diarrheagenic activities, both of which were found to be heat-labile. The permeability factor activity of this enterotoxin was neutralized by antisera prepared against crude E. coli enterotoxin, Vibrio cholerae enterotoxin (choleragen), and V. cholerae toxoid (choleragenoid), respectively. Supernatant fluids of 6-h E. coli cultures did not contain this molecular form of enterotoxin but did contain very high-molecular-weight, heat-labile enterotoxin. Incubation of cells derived from older (18 h) cultures with polymyxin caused the release of both low- (20,000) and high-molecular-weight forms of enterotoxin. We concluded that either the 20,000-dalton form of heat-labile enterotoxin is not released by E. coli under in vitro growth conditions or that enterotoxin released in this form is rapidly destroyed or inactivated.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    URL: Article
    DOI: 10.1128/iai.10.5.1010-1017.1974
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1974
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Diversity in the Variable Region ofHelicobacter pylori cagAGene Involves More Than Simple Repetition of a 102-Nucleotide Sequence
    Elsevier BV ; 1998
    In:  Biochemical and Biophysical Research Communications Vol. 245, No. 3 ( 1998-04), p. 780-784
    Details
    In: Biochemical and Biophysical Research Communications, Elsevier BV, Vol. 245, No. 3 ( 1998-04), p. 780-784
    Type of Medium: Online Resource
    ISSN: 0006-291X
    URL: Article
    DOI: 10.1006/bbrc.1998.8465
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1998
    detail.hit.zdb_id: 1461396-7
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...