In:
Yeast, Wiley, Vol. 31, No. 5 ( 2014-05), p. 159-166
Abstract:
Centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. It can, for instance, isolate G 1 daughter cells from a Saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell‐cycle biases when conducting genome‐wide/single‐cell studies. The present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast species can be efficiently elutriated, while offering solutions to optimize success. This approach was used to characterize elutriation parameters and S‐phase progression of four yeast species ( S. cerevisiae , Candida glabrata , Lachancea kluyveri and Pichia sorbitophila ) and could theoretically be applied to any culture of single, individual cells. Copyright © 2014 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0749-503X
,
1097-0061
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
1479172-9
SSG:
12
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