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  • 1
    In: Revista Brasileira de Farmacognosia, Springer Science and Business Media LLC, Vol. 25, No. 2 ( 2015-03), p. 134-141
    Type of Medium: Online Resource
    ISSN: 0102-695X
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2015
    detail.hit.zdb_id: 2690840-2
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  • 2
    In: Plants, MDPI AG, Vol. 12, No. 15 ( 2023-07-29), p. 2813-
    Abstract: Plant secondary metabolites are key components for new, safe and effective drugs. Ethanolic extract of Maesa indica Roxb. Sweet (ME) aerial parts were used for biosynthesis of sustainable green zinc oxide nanoparticles (ZnO NPs) with an average particle size 6.80 ± 1.47 nm and zeta potential −19.7 mV. Both transmission electron microscopy and X-ray diffraction assay confirmed the hexagonal shape of ZnO NPs. Phenolic ingredients in ME were identified using LC-ESI-MS/MS-MRM revealing the identification of chlorogenic acid, gallic acid, caffeic acid, rutin, coumaric acid, vanillin, naringenin, quercetin, ellagic acid, 3.4-dihydroxybenzoic acid, methyl gallate, kaempferol, ferulic acid, syringic acid, and luteolin. The major compound was chlorogenic acid at concentration of 1803.84 μg/g. The antiviral activity of ME, ZnO NPs, and combination of ME with ZnO NPs against coronavirus 229E were investigated. ZnO NPs had superior antiviral effect against coronavirus 229E than ME while their combination showed the highest anti-coronavirus 229E effect, with 50% inhibition concentration (IC50) of 5.23 ± 0.18 µg/mL and 50% cytotoxic concentration (CC50) of 138.49 ± 0.26 µg/mL while the selectivity index (SI) was 26.47. The current study highlighted the possible novel anti-coronavirus 229E activity of green ZnO NPs synthesized from Maesa indica. More studies are needed to further investigate this antiviral activity to be utilized in future biomedical and environmental applications.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2704341-1
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  • 3
    In: Antioxidants, MDPI AG, Vol. 8, No. 12 ( 2019-12-15), p. 646-
    Abstract: The prevalence of hepatic diseases globally and in Egypt particularly necessitates an intensive search for natural hepatoprotective candidates. Despite the traditional use of Chrysophyllum oliviforme L. and C. cainito L. leaves in the treatment of certain ailments, evidence-based reports on their bioactivities are limited. In this work, in vivo and in silico studies were conducted to evaluate their methanol extracts potential to alleviate liver damage in CCl4-intoxicated rats, in addition to their antioxidant activity and identifying the molecular mechanisms of their phenolic constituents. The extracts restored the altered total cholesterol (TC), triglycerides (TG), high-density lipoproteins (HDL), alanine aminotransferase ALT, aspartate aminotransferase AST, total protein, and albumin. Histopathological architecture, DNA fragmentation, and mRNA expression level of TGF-β1 also confirmed the anti-fibrotic activity of the two extracts. The total phenolic content (TPC) in C. oliviforme ethanol extract exceeded that in C. caimito. Additionally, the malondialdehyde (MDA), reduced glutathione (GSH), and total antioxidant capacity (TAC) levels assured the antioxidant potential. Seven phenolics; quercetin, isoquercitrin, myricetin, kaempferol, and caffeic, trans-ferulic, and gallic acids were isolated from the ethanol extract of C. oliviforme. The molecular docking of isolated compounds revealed a low binding energy (kcal/mol with TGF-β1, thus confirming the hepatoprotctive activity of the extracts. In conclusion, the C. oliviforme leaves could be considered as potent safe raw material for the production of herbal formulations to alleviate hepatic toxicity after preclinical safety study.
    Type of Medium: Online Resource
    ISSN: 2076-3921
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2704216-9
    SSG: 15,3
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  • 4
    In: Chemistry & Biodiversity, Wiley, Vol. 18, No. 7 ( 2021-07)
    Abstract: Plants belonging to Euphorbia L. genus are considered very interesting from a medicinal point of view due to their diverse metabolites and bioactivities. The essential oil (EO) of Euphorbia mauritanica L. is not studied up to date. Therefore, the present study aimed to explore the chemical profile of this EO and evaluate its antioxidant, cytotoxic, and allelopathic potentialities. The EO was extracted from the whole plant via hydrodistillation and then, analyzed by gas chromatography/mass spectrometry (GC/MS). The correlation of E. mauritanica with the other Euphorbia plants was established using chemometric analysis. The antioxidant activity was determined based on scavenging of the free radical, 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS). The anti‐proliferation of the EO on the Hep G2 and MCF‐7 cells was evaluated. Finally the allelopathic activity of the EO was assessed against the two noxious weeds, Dactyloctenium aegyptium and Urospermum picroides . Forty‐one compounds were identified using GC/MS analysis, with an abundance of terpenoids (91.54 %) that were categorized into mono‐ (30.75 %), sesqui‐ (15.23 %), and diterpenes (45.56 %). Interestingly, the results revealed the preponderance of diterpenoid constituents although they are rarely found in the EOs of the plant kingdom. The major compounds were (3 E )‐cembrene A (18.66 %), verticiol (17.05 %), limonene (7.91 %), eucalyptol (7.26 %), α‐pinene (5.61 %), neo‐cembrene A (3.52 %), kaur‐16‐ene (3.24 %), and cembrene (3.09 %). The EO showed moderate antioxidant activity where it attained IC 50 values of 83.34 and 64.21 μg mL −1 for DPPH and ABTS compared to 23.01 and 19.23 μg mL −1 for ascorbic acid as standard, respectively. The EO exhibited very weak cytotoxic effect on MCF‐7 and Hep G2 cells. The EO showed significant allelopathic activities against the weeds D. aegyptium and U. picroides in a concentration‐dependent manner. EO was found more effective against U. picroides than D. aegyptium with IC 50 values of 0.79, 0.45, and 0.67 mg mL −1 and 1.17, 0.55, and 1.08 mg mL −1 for germination, root, and shoot growth, respectively. Due to the high content of diterpenes in E. mauritanica , further study is recommended for more characterization of pure forms of the identified diterpenes as well as evaluating their bioactivity either solely or synergistically.
    Type of Medium: Online Resource
    ISSN: 1612-1872 , 1612-1880
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2139001-0
    SSG: 12
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  • 5
    In: Chemistry & Biodiversity, Wiley, Vol. 20, No. 2 ( 2023-02)
    Abstract: In spite of tremendous efforts exerted in the management of COVID‐19, the absence of specific treatments and the prevalence of delayed and long‐term complications termed post‐COVID syndrome still urged all concerned researchers to develop a potent inhibitor of SARS‐Cov‐2. The hydromethanolic extracts of different parts of E. mauritanica were in vitro screened for anti‐SARS‐Cov‐2 activity. Then, using an integrated strategy of LC/MS/MS, molecular networking and NMR, the chemical profile of the active extract was determined. To determine the optimum target for these compounds, docking experiments of the active extract's identified compounds were conducted at several viral targets. The leaves extract showed the best inhibitory effect with IC 50 8.231±0.04 μg/ml. The jatrophane diterpenes were provisionally annotated as the primary metabolites of the bioactive leaves extract based on multiplex of LC/MS/MS, molecular network, and NMR. In silico studies revealed the potentiality of the compounds in the most active extract to 3CLpro, where compound 20 showed the best binding affinity. Further attention should be paid to the isolation of various jatrophane diterpenes from Euphorbia and evaluating their effects on SARS‐Cov‐2 and its molecular targets.
    Type of Medium: Online Resource
    ISSN: 1612-1872 , 1612-1880
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2139001-0
    SSG: 12
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  • 6
    In: RSC Advances, Royal Society of Chemistry (RSC), Vol. 11, No. 27 ( 2021), p. 16755-16767
    Type of Medium: Online Resource
    ISSN: 2046-2069
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2021
    detail.hit.zdb_id: 2623224-8
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  • 7
    Online Resource
    Online Resource
    Mary Ann Liebert Inc ; 2013
    In:  Journal of Medicinal Food Vol. 16, No. 11 ( 2013-11), p. 1022-1029
    In: Journal of Medicinal Food, Mary Ann Liebert Inc, Vol. 16, No. 11 ( 2013-11), p. 1022-1029
    Type of Medium: Online Resource
    ISSN: 1096-620X , 1557-7600
    Language: English
    Publisher: Mary Ann Liebert Inc
    Publication Date: 2013
    detail.hit.zdb_id: 2030886-3
    SSG: 15,3
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  • 8
    In: International Journal of Applied Pharmaceutics, Innovare Academic Sciences Pvt Ltd, ( 2019-09-23), p. 154-164
    Abstract: Objective: Evaluation of two Jasminum sambac L. (Ait) cultivars; Arabian Nights (JSA) and Grand Duke of Tuscany (JSG) ethanolic leaves extracts as reducing agents for the green synthesis of silver nanoparticles (AgNPs) and evaluation of their cytotoxicity against MCF-7 breast cancer and 5637 bladder cancer cell lines and chemical profiling of the two cultivars. Methods: The synthesis of silver nanoparticles (AgNPs) by the two cultivars and characterization of AgNPs by ultraviolet (UV)–visible spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). Additionally, the use of The high-performance liquid chromatography coupled with photodiode array-mass-mass-spectroscopy (HPLC-PDA-MS/MS) for chemical profiling of both cultivars and evaluation of total leaves extracts and corresponding nanoparticles towards MCF-7 and 5637 cell lines compared to aneuploidy immortal keratinocyte (Ha Cat) normal cells by neutral cell assay. Results: The green synthesized AgNPs (of an average size range of 8.83 and 11.24 nm for JSA and JSG, respectively) exhibited cytotoxicity against MCF-7 and 5637 cell lines. The IC50 was determined for each total extract JSA (15.29±2.16 μg/ml) and JSG (20.28±1.20 μg/ml) and corresponding AgNPs 17.32±2.22 μg/ml and 6.32±1.01μg/ml for JSA and JSG, respectively. The IC50 of JSA and JSG against 5637 bladder cancer cell line were 13.76±1.11 μg/ml and 50.69±3.75 μg/ml, while the corresponding AgNPs showed IC50 of 5.54±0.88 μg/ml and 27.89±2.84 μg/ml, respectively. The HPLC-PDA-MS/MS allowed the identification of 59 compounds; 10 simple phenols, 17 flavonoids; quercetin and kaempferol glycosides, 2 lignans, and 30 secoiridoids; oleuropein, molihauside, and sambacoside. Conclusion: This study proved that JSA is an excellent source for the synthesis of AgNPs with optimum characters and enhanced activities toward MCF-7 and 5637 cell lines in correlation to identified compounds.
    Type of Medium: Online Resource
    ISSN: 0975-7058
    Language: Unknown
    Publisher: Innovare Academic Sciences Pvt Ltd
    Publication Date: 2019
    detail.hit.zdb_id: 2675577-4
    SSG: 15,3
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  • 9
    Online Resource
    Online Resource
    Sociedad Quimica de Mexico, A.C. ; 2022
    In:  Journal of the Mexican Chemical Society Vol. 66, No. 4 ( 2022-10-01)
    In: Journal of the Mexican Chemical Society, Sociedad Quimica de Mexico, A.C., Vol. 66, No. 4 ( 2022-10-01)
    Abstract: Abstract. Tecoma genus is a medicinally-valuable member in Bignoniaceae family comprising fourteen defectively-demarcated species with overlapping vegetative characters. Our study aimed to explore the micro-morphometric, phytochemical and genetic diversity among eight cultivated Tecoma plants. The variabilities existing in three data matrices derived from micro-morphometric features of powdered leaves, high performance liquid chromatographic fingerprints and Random Amplified Polymorphic DNA (RAPD) fingerprints were explored by multivariate analysis. The microscopical measurements of non-glandular trichomes, i.e. collapsed multicellular and branched, and glandular trichomes were revealed to be the significant botanical discriminatory features of the powdered plant specimens, however species-specific phytochemical markers were identified to be gallic acid, catechin and chlorogenic acid for T. radicans, T. grandiflora, T. capensis var. yellow, respectively. Sinapic acid was enriched in T capensis and its varieties i.e. pink, red and harmony. RAPD primers i.e. OP-B02, OP-C04 and OP-K2 served as molecular descriptors producing the highest polymorphism among the plants. The variabilities within two trumpet creeper plants “T. radicans and T. grandiflora” were unveiled via their micro-morphometric features as well as genetic fingerprints. Tecoma hybrid “Tecoma ×smithii” was more micro-morphometrically similar to the Chinese “T. grandiflora.” than T. capensis which is one of its parent plants, and proved to be more genetically related to it. This is the report for biodiversity in botanical, chemical and genetic aspects of Tecoma plants which are essential for the comprehensive authentication and differentiation among the studied plants assisted by multivariate analysis. The presented approach provides a competent and time-saving tool for simultaneously-discriminating among closely-related species.   Resumen. El género Tecoma es un miembro medicinalmente valioso de la familia Bignoniaceae que comprende catorce especies demarcadas defectuosamente con caracteres vegetativos superpuestos. Nuestro estudio tuvo como objetivo explorar la diversidad micromorfométrica, fitoquímica y genética entre ocho plantas de Tecoma cultivadas. Se exploraron mediante análisis multivariado las variabilidades existentes en tres matrices de datos derivadas de las características micromorfométricas de las hojas en polvo, las huellas dactilares por medio de cromatografía líquida de alta resolución y las huellas dactilares de ADN polimórfico amplificado al azar (RAPD). Las mediciones microscópicas de tricomas no glandulares, esto es, tricomas multicelulares colapsados ​​y ramificados, y tricomas glandulares mostraron ser las características significativas de discriminación botánica de las muestras en polvo de plantas. Se identificaron marcadores fitoquímicos específicos para algunas especies como ácido gálico, catequina y ácido clorogénico para T. radicans, T. grandiflora, T. capensis var. amarillo, respectivamente. El ácido sinápico está enriqueció en T capensis y sus variedades, esto es, rosa, rojo y armonía. Los cebadores RAPD, esto es, OP-B02, OP-C04 y OP-K2 sirvieron como descriptores moleculares que producen el polimorfismo más elevado entre las plantas. A través de sus características micromorfométricas y sus huellas genéticas se determinó la variabilidad en dos plantas trepadoras de trompeta “T. radicans y T. grandiflora.” El híbrido de Tecoma “Tecoma × smithii” es micromorfométricamente más similar al chino “T. grandiflora” que a T. capensis, que es una de sus plantas progenitoras, y mostró estar más relacionada genéticamente con ella. En esta investigación de biodiversidad en aspectos botánicos, químicos y genéticos de las plantas de Tecoma que son esenciales para la autenticación y diferenciación integral entre las plantas estudiadas con la ayuda del análisis multivariado. El enfoque presentado proporciona una herramienta eficiente y que ahorra tiempo para discriminar simultáneamente entre especies estrechamente relacionadas.
    Type of Medium: Online Resource
    ISSN: 2594-0317 , 1870-249X
    Language: Unknown
    Publisher: Sociedad Quimica de Mexico, A.C.
    Publication Date: 2022
    detail.hit.zdb_id: 2210387-9
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  • 10
    In: RSC Advances, Royal Society of Chemistry (RSC), Vol. 10, No. 70 ( 2020), p. 43103-43108
    Type of Medium: Online Resource
    ISSN: 2046-2069
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2020
    detail.hit.zdb_id: 2623224-8
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