In:
Journal of Bacteriology, American Society for Microbiology, Vol. 180, No. 4 ( 1998-02-15), p. 809-814
Abstract:
The gene rapL lies within the region of the Streptomyces hygroscopicus chromosome which contains the biosynthetic gene cluster for the immunosuppressant rapamycin. Introduction of a frameshift mutation into rapL by ΦC31 phage-mediated gene replacement gave rise to a mutant which did not produce significant amounts of rapamycin. Growth of this rapL mutant on media containing added l -pipecolate restored wild-type levels of rapamycin production, consistent with a proposal that rapL encodes a specific l -lysine cyclodeaminase important for the production of the l -pipecolate precursor. In the presence of added proline derivatives, rapL mutants synthesized novel rapamycin analogs, indicating a relaxed substrate specificity for the enzyme catalyzing pipecolate incorporation into the macrocycle.
Type of Medium:
Online Resource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/JB.180.4.809-814.1998
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1998
detail.hit.zdb_id:
1481988-0
SSG:
12
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