In:
Small, Wiley, Vol. 19, No. 31 ( 2023-08)
Abstract:
Nucleolus, which participates in many crucial cellular activities, is an ideal target for evaluating the state of a cell or an organism. Here, bright red‐emissive carbon dots (termed CPCDs) with excitation‐independent/polarity‐dependent fluorescence emission are synthesized by a one‐step hydrothermal reaction between congo red and p ‐phenylenediamine. The CPCDs can achieve wash‐free, real‐time, long‐term, and high‐quality nucleolus imaging in live cells, as well as in vivo imaging of two common model animals—zebrafish and Caenorhabditis elegans ( C . elegans ). Strikingly, CPCDs realize the nucleolus imaging of organs/flowing blood cells in zebrafish at a cellular level for the first time, and the superb nucleolus imaging of C . elegans suggests that the germ cells in the spermatheca probably have no intact nuclei. These previously unachieved imaging results of the cells/tissues/organs may guide the zebrafish‐related studies and benefit the research of C . elegans development. More importantly, a novel strategy based on CPCDs for in vivo toxicity evaluation of materials/drugs (e.g., Ag + ), which can visualize the otherwise unseen injuries in zebrafish, is developed. In conclusion, the CPCDs represent a robust tool for visualizing the structures and dynamic behaviors of live zebrafish and C . elegans , and may find important applications in cell biology and toxicology.
Type of Medium:
Online Resource
ISSN:
1613-6810
,
1613-6829
DOI:
10.1002/smll.202205890
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2168935-0
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