In:
PeerJ, PeerJ, Vol. 9 ( 2021-02-25), p. e10892-
Abstract:
The Doublesex/Mab-3 Domain transcription factor DMD-10 is expressed in several cell types in C. elegans , including in the nervous system. We sought to investigate whether DMD-10 is required for normal neuronal function using behavioral assays. We found that mutation of dmd-10 did not broadly affect behavior. dmd-10 mutants were normal in several behavioral assays including a body bends assay for locomotion, egg laying, chemotaxis and response to gentle touch to the body. dmd-10 mutants did have defects in nose-touch responsiveness, which requires the glutamate receptor GLR-1. However, using quantitative fluorescence microscopy to measure levels of a GLR-1::GFP fusion protein in the ventral nerve cord, we found no evidence supporting a difference in the number of GLR-1 synapses or in the amount of GLR-1 present in dmd-10 mutants. dmd-10 mutants did have decreased responsiveness to high osmolarity, which, along with nose-touch, is sensed by the polymodal sensory neuron ASH. Furthermore, mutation of dmd-10 impaired behavioral response to optogenetic activation of ASH, suggesting that dmd-10 promotes neuronal signaling in ASH downstream of sensory receptor activation. Together our results suggest that DMD-10 is important in regulating the frequency of multiple ASH-dependent behavioral responses.
Type of Medium:
Online Resource
ISSN:
2167-8359
DOI:
10.7717/peerj.10892/fig-1
DOI:
10.7717/peerj.10892/fig-2
DOI:
10.7717/peerj.10892/fig-3
DOI:
10.7717/peerj.10892/fig-4
DOI:
10.7717/peerj.10892/fig-5
DOI:
10.7717/peerj.10892/fig-6
DOI:
10.7717/peerj.10892/supp-1
DOI:
10.7717/peerj.10892/supp-2
DOI:
10.7717/peerj.10892/supp-3
DOI:
10.7717/peerj.10892/supp-4
DOI:
10.7717/peerj.10892/supp-5
Language:
English
Publisher:
PeerJ
Publication Date:
2021
detail.hit.zdb_id:
2703241-3
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