In:
Parasitology, Cambridge University Press (CUP), Vol. 145, No. 3 ( 2018-03), p. 292-306
Abstract:
Immunoactivation depends upon the antigen potential to modulate T-cell repertoires. The present study has enumerated the effect of 61 kDa recombinant Leishmania donovani co-factor-independent phosphoglycerate mutase (r Ld -iPGAM) on mononuclear cells of healthy and treated visceral leishmaniasis subjects as well as on THP-1 cell line. r Ld -iPGAM stimulation induced higher expression of interleukin-1 β (IL-1 β ) in the phagocytic cell, its receptor and CD69 on T-cell subsets. These cellular activations resulted in upregulation of host-protective cytokines IL-2, IL-12, IL-17, tumour necrosis factor- α and interferon- γ , and downregulation of IL-4, IL-10 and tumour growth factor- β . This immune polarization was also evidenced by upregulation of nuclear factor- κ light-chain enhancer of activated B cells p50 and regulated expression of suppressor of mother against decapentaplegic protein-4. r Ld -iPGAM stimulation also promoted lymphocyte proliferation and boosted the leishmaniacidal activity of macrophages by upregulating reactive oxygen species. It also induced 1·8-fold higher release of nitric oxide (NO) by promoting the transcription of inducible nitric oxide synthase gene. Besides, in silico analysis suggested the presence of major histocompatibility complex class I and II restricted epitopes, which can proficiently trigger CD8 + and CD4 + cells, respectively. This study reports r Ld -iPGAM as an effective immunoprophylactic agent, which can be used in future vaccine design.
Type of Medium:
Online Resource
ISSN:
0031-1820
,
1469-8161
DOI:
10.1017/S0031182017001494
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2018
detail.hit.zdb_id:
1491287-9
detail.hit.zdb_id:
207627-5
SSG:
12
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