In:
Microbial Physiology, S. Karger AG, Vol. 14, No. 1-3 ( 2008), p. 128-136
Abstract:
The survival of 〈 i 〉 Bifidobacterium animalis 〈 /i 〉 strain DN-173 010 was assessed after its ingestion in a fermented product or in a lyophilised form. Twelve healthy subjects were included in a randomised, open study with 2 parallel groups. The composition and activities of the faecal microbiota were monitored before (10-day baseline step), during (1-week product administration step) and after (10-day follow-up step) the ingestion of 1 of the 2 products. A colony immunoblotting method, fluorescent in situ hybridisation with group-specific DNA probes, and temporal temperature gradient gel electrophoresis using group-specific primers were carried out to compare survival of 〈 i 〉 B. animalis 〈 /i 〉 strain DN-173 010 after ingestion of the 2 products, together with analyses of enzyme activities and faecal metabolites. At the end of the supplementation step, the mean number of 〈 i 〉 B. animalis 〈 /i 〉 DN-173 010 quantified by immunodetection in the faeces of 5 of 6 subjects in each treatment group was ≧10 〈 sup 〉 8 〈 /sup 〉 colony-forming units/g faeces. These numbers corresponded to an average survival of 22% for the lyophilised form and 20% for the fermented product. At the same step, the PCR temporal temperature gradient gel electrophoresis profiles showed a double band corresponding to the 〈 i 〉 B. animalis 〈 /i 〉 DN-173 010 pattern for 11 subjects. No major modification was observed during the trial in either the dominant members of the faecal microbiota assessed by fluorescent in situ hybridisation or their activities. In conclusion, we show that the lyophilised form of 〈 i 〉 B. animalis 〈 /i 〉 DN-173 010 survives transit and could represent a more convenient form to administer for long-term clinical trials.
Type of Medium:
Online Resource
ISSN:
2673-1665
,
2673-1673
Language:
English
Publisher:
S. Karger AG
Publication Date:
2008
detail.hit.zdb_id:
3042601-7
detail.hit.zdb_id:
3008505-6
Permalink