In:
Journal of Occupational Health, Wiley, Vol. 53, No. 4 ( 2011-07), p. 250-257
Abstract:
Injury of Cell Tight Junctions and Changes of Actin Level in Acute Lung Injury Caused by the Perfluoroisobutylene Exposure and the Role of Myosin Light Chain Kinase: Ge Meng, et al. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, P. R. China— Objectives To investigate the injury of cell tight junctions and change in actin level in the alveolus epithelial cells of the lung after Perfluoroisobutylene (PFIB) exposure and the role of myosin light chain kinase (MLCK) in the injury. Methods Rats and mice were exposed to a sublethaldose of PFIB. The changes in tight junction zonula occludens‐1 (ZO‐1), actin and myosin light chain kinase (MLCK) were detected by immunoluorescence at 30 min, 1, 2, 4, 8, 16, 24, 48 and 72 h after PFIB exposure. The role of MLCK was analyzed by lung indices and the act in leve l. Resu lts: The norma l ZO‐1 immunofluorescence density and those after PFIB exposure were 71.63, 39.41, 37.59, 35.71, 33.22, 31.34,31.61, 24.51, 40.03 and 44.71 respectively, The normal actin immunoluorescence density and those after PFIB exposure were 31.82, 36.46, 36.57, 41.60, 40.95, 35.41,30.69, 19.96, 29.30 and 33.00 respectively, The normal MLCK immunoluorescence density and those after PFIB exposure were 61.21, 50.87, 48.37, 43.65, 41.96,35.44, 31.77, 30.85, 33.10 and 38.20 respectively. When the MLCK inhibitor ML‐7 was given in advance, pulmonary edema and actin degradation were suppressed. Conclusions At an earlier stage, the increased permeability of the blood‐air barrier after PFIB exposure is probably the result of injury of cell tight junctions that acts in concert with later changes in actin, resulting in an increase in permeability. MLCK could be a potential target for novel drug development for relief of acute lung injury.
Type of Medium:
Online Resource
ISSN:
1341-9145
,
1348-9585
DOI:
10.1539/joh.10-0055-OA
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
1340985-2
detail.hit.zdb_id:
2075956-3
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