In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 14, No. 12 ( 2020-12-4), p. e0008942-
Abstract:
Many ruminant diseases of viral aetiology can be effectively prevented using appropriate vaccination measures. For diseases such as Rift Valley fever (RVF) the long inter-epizootic periods make routine vaccination programs unfeasible. Coupling RVF prophylaxis with seasonal vaccination programmes by means of multivalent vaccine platforms would help to reduce the risk of new RVF outbreaks. Methodology/Principal findings In this work we generated recombinant attenuated Rift Valley fever viruses (RVFVs) encoding in place of the virulence factor NSs either the VP2 capsid protein or a truncated form of the non-structural NS1 protein of bluetongue virus serotype 4 (BTV-4). The recombinant viruses were able to carry and express the heterologous BTV genes upon consecutive passages in cell cultures. In murine models, a single immunization was sufficient to protect mice upon RVFV challenge and to elicit a specific immune response against BTV-4 antigens that was fully protective after a BTV-4 boost. In sheep, a natural host for RVFV and BTV, both vaccines proved immunogenic although conferred only partial protection after a virulent BTV-4 reassortant Morocco strain challenge. Conclusions/Significance Though additional optimization will be needed to improve the efficacy data against BTV in sheep, our findings warrant further developments of attenuated RVFV as a dual vaccine platform carrying heterologous immune relevant antigens for ruminant diseases in RVF risk areas.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0008942
DOI:
10.1371/journal.pntd.0008942.g001
DOI:
10.1371/journal.pntd.0008942.g002
DOI:
10.1371/journal.pntd.0008942.g003
DOI:
10.1371/journal.pntd.0008942.g004
DOI:
10.1371/journal.pntd.0008942.g005
DOI:
10.1371/journal.pntd.0008942.g006
DOI:
10.1371/journal.pntd.0008942.g007
DOI:
10.1371/journal.pntd.0008942.g008
DOI:
10.1371/journal.pntd.0008942.g009
DOI:
10.1371/journal.pntd.0008942.s001
DOI:
10.1371/journal.pntd.0008942.s002
DOI:
10.1371/journal.pntd.0008942.s003
DOI:
10.1371/journal.pntd.0008942.s004
DOI:
10.1371/journal.pntd.0008942.s005
DOI:
10.1371/journal.pntd.0008942.s006
DOI:
10.1371/journal.pntd.0008942.s007
DOI:
10.1371/journal.pntd.0008942.s008
DOI:
10.1371/journal.pntd.0008942.s009
DOI:
10.1371/journal.pntd.0008942.r001
DOI:
10.1371/journal.pntd.0008942.r002
DOI:
10.1371/journal.pntd.0008942.r003
DOI:
10.1371/journal.pntd.0008942.r004
DOI:
10.1371/journal.pntd.0008942.r005
DOI:
10.1371/journal.pntd.0008942.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2429704-5
Permalink