GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Quantitative Trait Loci (QTL) Detection and Validation of Linked Markers Associated with Yellow Mosaic Virus Resistance in Urdbean [Vigna mungo (L.) Hepper] in Andhra Pradesh
    Agricultural Research Communication Center ; 2022
    In:  Indian Journal Of Agricultural Research , No. Of ( 2022-4-30)
    Details
    In: Indian Journal Of Agricultural Research, Agricultural Research Communication Center, , No. Of ( 2022-4-30)
    Abstract: Background: The YMD is one of the serious viral diseases in blackgram and is being transmitted by whitefly (Bemisia tabaci). The development of YMV resistant blackgram varieties is one of the important aspects for sustainable blackgram production. Marker assisted selection and genetic transformation could be utilized in developing YMV resistant blackgram genotypes. Methods: We attempted to map the QTLs governing YMV resistance in blackgram employing parents i.e., PBG32 (susceptible) and PU31 (resistant). Parental polymorphic studies were conducted using 147 SSR markers and genotyping and phenotyping data were used for QTL mapping. The markers, CEDG097 and CEDG172 that flanked to qYMV1 QTL were validated in 25 blackgram genotypes. Result: SSR markers showed 16% polymorphism between the parents. By using F2 genotypic data, genetic linkage map was constructed and total genetic map length observed was 335.47 cm. Single QTL (qYMV1) for YMV tolerance was detected and located between markers CEDG097 and CEDG172 with LOD score value of 2.76. The PVE by qYMV1 is 13.10% and the QTL is tightly linked (0 cM) to the left flanking marker CEDG97, which denotes a major QTL. Genotyping of the 25 known blackgram genotypes employing CEDG097 and CEDG172 markers, CEDG097 showed 91% linkage in known resistant and susceptible varieties to the YMV disease reaction and CEDG172 exhibited 74% linkage in known varieties to the YMV disease reaction. The resistance alleles (PU31 allele) of both markers i.e. CEDG097 and CEDG172 were recorded in VBN7, LBG922, LBG933 and TBG130-1 hence, these genotypes can be considered as potent YMV resistant varieties and also can be used as donor parents in resistance breeding programmes.
    Type of Medium: Online Resource
    ISSN: 0976-058X , 0367-8245
    URL: Article
    DOI: 10.18805/IJARe.A-5895
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2022
    detail.hit.zdb_id: 2569845-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Genetic Variability Studies of Yellow Mosaic Virus Infecting Blackgram [Vigna mungo (L.) Hepper] from Andhra Pradesh, India
    Agricultural Research Communication Center ; 2022
    In:  LEGUME RESEARCH - AN INTERNATIONAL JOURNAL , No. Of ( 2022-3-28)
    Details
    In: LEGUME RESEARCH - AN INTERNATIONAL JOURNAL, Agricultural Research Communication Center, , No. Of ( 2022-3-28)
    Abstract: Background: The productivity of blackgram is affected by many biotic and abiotic stresses. Among the biotic stresses, yellow mosaic disease (YMD) cause severe yield loss and it is caused by four distinct viruses (belongs to genus begomovirus) collectively known as yellow mosaic virus (YMV). Hence there is need to characterize various YMV isolates associated with blackgram in Andhra Pradesh. Methods: YMV infected blackgram samples were collected from East Godavari, Kurnool and Prakasam districts of Andhra Pradesh. The Rolling Circle Amplification (RCA) based full length MYMIV DNA-A and MYMV DNA-B of three isolates were cloned and sequenced. Result: Nucleotide sequence of full length DNA-A of MYMIV-East Godavari isolate showed greater than 96% similarity at nucleotide and greater than 90% at amino acid level with other MYMIV isolates in NCBI database. The complete DNA-A nucleotide sequence of MYMIV-Kurnool and MYMIV-Prakasam isolates shared greater than 99% similarity at nucleotide and greater than 98% at amino acid level with other MYMIV isolates. The complete nucleotide sequence of MYMV DNA-B of three isolates (East Godavari, Kurnool and Prakasam) had greater than 97% homology with other MYMV DNA-B isolates from database. The predicted amino acid sequence of MYMV DNA-B of three isolates shared greater than 96% homology with other MYMV-B isolates. The common region (CR) sequence similarity between MYMIV-As and MYMV-Bs of East Godavari and Kurnool isolates was 76.4% and 78.3% with Prakasam isolate. The divergence between the MYMIV-A and MYMV-B of present three isolates (East Godavari, Kurnool and Prakasam) under study were ranged from 22.2 to 22.6%.
    Type of Medium: Online Resource
    ISSN: 0976-0571 , 0250-5371
    URL: Article
    DOI: 10.18805/LR-4800
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Molecular characterization of virus species causing yellow mosaic disease of redgram in Andhra Pradesh
    EM International, Publishers of Quality International Journals ; 2022
    In:  Ecology, Environment and Conservation ( 2022-5-31), p. 300-305
    Details
    In: Ecology, Environment and Conservation, EM International, Publishers of Quality International Journals, ( 2022-5-31), p. 300-305
    Abstract: Redgram is animportant drought tolerant leguminous crop in the semi-arid subtropics. Many fungal, bacterialand viral diseases are major constraints for redgram production, apart from all these diseases, yellowmosaic disease (YMD) is observed in redgram crop in few localities of Chittoor district, Andhra Pradeshduring 2017. The present study was aimed to find out associated begomo virus species causing YMD inredgram.The total genomic DNA was isolated from YMV infected redgram leaves by modified CTAB method.The rolling circle amplification based full length clones were constructed in pUC18 vector. The two constructed clones (DNA-A and DNA-B) were confirmed by using gene specific primers and weresequenced.The coat protein gene andcomplete nucleotide sequence analysis was carried out with the knownbegomoviruses sequences obtained from Genbank database.The phylogenetic analysis of coat protein geneof YMV-AP-Red gram isolate shared 94.3-98.1% nucleotide identity with HgYMV isolates, followed byMYMV (81.2-85.2%), MYMIV (78.1-80.2%) and DoYMV(72.2-74%). Hence it was confirmed that viral speciescausing YMD in redgram from Tirupati, AndhraPradesh is a Horsegram yellow mosaic virus and it is avariant of HgYMV as it shared 〉 94% similarity with other HgYMV isolates at nucleotide level.
    Type of Medium: Online Resource
    ISSN: 0971-765X
    URL: Journal
    URL: Article
    DOI: 10.53550/EEC
    DOI: 10.53550/EEC.2022.v28i03s.045
    Language: Unknown
    Publisher: EM International, Publishers of Quality International Journals
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Distribution of Two Species of Begomoviruses Infecting Blackgram in Andhra Pradesh
    Sciencedomain International ; 2020
    In:  Current Journal of Applied Science and Technology ( 2020-11-27), p. 12-17
    Details
    In: Current Journal of Applied Science and Technology, Sciencedomain International, ( 2020-11-27), p. 12-17
    Abstract: Background: Yellow mosaic diseases of blackgram is caused by two species of begomoviruses (MYMV, MYMIV). It is known fact that certain varities which are resistant to one species are susceptible to another species when screened by agroinoculations. Hence data on distribution of DNA-A & B components of MYMV / MYMIV under field conditions were not available in Andhra Pradesh. Methods: In the present study total eighty yellow mosaic infected blackgram samples were colleted from seven districts of Andhra Pradesh during rabi and kharif   2016-17. The total DNA was isolated and detected for presence DNA-A and B components of   MYMV/ MYMIV by PCR with specific primers. Samples which showed negative in normal PCR are further detected by RCA-PCR. Results: PCR results showed that out of 80 YMD infected blackgram samples tested from seven districts, positve results were otained in 50% samples for MYMV-A, 98.75% for MYMIV-A,and  90% for MYMV-B   and none for MYMIV-B. However RCA-PCR detected MYMIV-B presence in all 7 samples (8.25%) collected from Guntur district, but none from other six districts. These results clearly shows that both species of begoviruses are present in Andhra Pradesh and MYMIV-A is predominant species   in Andhra Pradesh as compared to MYMV-A. Hence germplasm and breedig material should be screened for both the species of begomovirses either under field or through agroinoculations to develop YMV resistant blackgram. 
    Type of Medium: Online Resource
    ISSN: 2457-1024
    URL: Article
    DOI: 10.9734/cjast/2020/v39i3631068
    Language: Unknown
    Publisher: Sciencedomain International
    Publication Date: 2020
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Biological characterization and host range of Tobacco Streak Virus (TSV)
    Agricultural Research Communication Center ; 2018
    In:  LEGUME RESEARCH - AN INTERNATIONAL JOURNAL , No. of ( 2018-11-26)
    Details
    In: LEGUME RESEARCH - AN INTERNATIONAL JOURNAL, Agricultural Research Communication Center, , No. of ( 2018-11-26)
    Abstract: TSV isolates (GNAP7. SFAP17 and CUKA13) maintained on cowpea were cross inoculated on five popularly grown varieties and hybrids of groundnut (JL-24, K-6, Prasuna, K-9, Kadiri harithandra), sunflower (KBSH-44, DRSH-1, NDSH-1012, Mordent, Sunbred-275) and cucumber (Long green, Poorna, Ageti, Sheetal, Uday). Incubation period required for the initiation of local and systemic symptoms varied among different hosts and cultivars. Local symptoms of veinal necrosis, leaf yellowing and wilting, petiole necrosis, chlorotic spot, tip necrosis and systemic symptoms of mosaic, wilting, necrotic streaks on stem, bud necrosis, axillary shoot proliferation were observed in different hosts and Culivars. Out of 23 plant species tested against TSV by sap inoculation, 15 plant species were susceptible to TSV, which includes Beta vulgaris, Abelmoschus esculentus, Cicer arietinum, Solanum melongena, Ricinus communis, Chrysanthemum indicum, Phaseolus vulgaris, Gerbera jamesonii, Macrotyloma uniflorum, Vigna unguiculata subsp. unguiculata, Tagetus erecta, Allium cepa, Phaseolus coccineus, Solanum lycopersicum, Vigna unguiculata ssp. sesquipedalis.
    Type of Medium: Online Resource
    ISSN: 0976-0571 , 0250-5371
    URL: Article
    DOI: 10.18805/LR-3934
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2018
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Construction of Full-length Dimer Clones of Yellow Mosaic Virus and Screening of Blackgram Germplasm using Agroinoculation
    Agricultural Research Communication Center ; 2022
    In:  LEGUME RESEARCH - AN INTERNATIONAL JOURNAL , No. Of ( 2022-1-18)
    Details
    In: LEGUME RESEARCH - AN INTERNATIONAL JOURNAL, Agricultural Research Communication Center, , No. Of ( 2022-1-18)
    Abstract: Background: Vigna mungo (Blackgram) is the major pulse crop cultivated in the Indian sub-continent. It is highly prone to yellow mosaic disease (YMD), which is widespread problem because all cultivated varieties are susceptible to the YMD in Andhra Pradesh state. The presence of two species of begomovirus i.e. Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV) with YMD was reported in Andhra Pradesh. The current study was aimed to develop simple method of viral dimer clone construction and identifying the resistant blackgram genotypes against two species of begomovirus (MYMV and MYMIV) by agroinoculation technique. Methods: In this study, we standardize the rolling circle amplification (RCA) based viral dimer clone construction (MYMV DNA-A, MYMV DNA-B and MYMIV DNA-A) and constructed viral dimer clones were used to transform Agrobacterium tumefaciens EHA105 cells through freeze-thaw technique. Sprouted seed method of agroinoculation screening was conducted. Result: Total 45 blackgram genotypes were screened by sprouted seed method of agroinoculation with two combinations of viral dimeric constructs (MYMV-TPT-A+MYMV-TPT-B and MYMIV-TPT-A+MYMV-TPT-B). Twenty four genotypes offered resistant against MYMV infection and twenty three genotypes were resistant to MYMIV infection. Total 17 genotypes offered resistance to both species of virus (MYMV andMYMIV) associated with YMD.
    Type of Medium: Online Resource
    ISSN: 0976-0571 , 0250-5371
    URL: Article
    DOI: 10.18805/LR-4775
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Biochemical characterization of Actinomycetes isolated from rhizosphere soils of groundnut in Andhra Pradeh
    EM International, Publishers of Quality International Journals ; 2023
    In:  Ecology, Environment and Conservation Vol. 29, No. 01 ( 2023), p. 342-346
    Details
    In: Ecology, Environment and Conservation, EM International, Publishers of Quality International Journals, Vol. 29, No. 01 ( 2023), p. 342-346
    Abstract: Roving survey was conducted and collected Actinomycetes isolates in major groundnut growing areas of Andhra Pradesh (10 districts) viz., Anantapuramu, YSR Kadapa, Kurnool, Chittoor, SPSR Nellore, Prakasam, Guntur, West Godavari, Vizianagaram and Srikakulam during Kharif, 2016. During the survey, 180 rhizospheric soil samples were collected and isolated a total number of fifty morphologically different Actinomycetes isolates. Basic identification of Actinomycetes was done by visual observation, general morphology, spore formation, colony morphology, an earthy odouri and including Gram stain. The isolates were characterized based on different biochemical tests. The enzymativ activity of isolates was studied to know the efficacy against stem rot pathogen Sclerotium rolfsii in groundnut.
    Type of Medium: Online Resource
    ISSN: 0971-765X
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.53550/EEC.2023
    DOI: 10.53550/EEC
    DOI: 10.53550/EEC.2023.v29i01.049
    Language: Unknown
    Publisher: EM International, Publishers of Quality International Journals
    Publication Date: 2023
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Morphological and cultural characterization of colletotrichum capsici, incitant of blight of chickpea in Andhra Pradesh, India
    Agricultural Research Communication Center ; 2016
    In:  Legume Research - An International Journal , No. OF ( 2016-04-09)
    Details
    In: Legume Research - An International Journal, Agricultural Research Communication Center, , No. OF ( 2016-04-09)
    Abstract: In Andhra Pradesh, Chickpea is severely affected by wilt (Fusarium oxysporum f.sp. ciceri) and dry root rot (Rhizoctonia bataticola). In addition to these two diseases, Colletotrichum blight occurred in severe form during rabi 2009 and 2010 due to heavy unusual rains, which resulted in crop failure in many areas leading to re-sowing of the crops. Hence studies were conducted on Colletotrichum blight of chickpea, where a total of seven isolates were collected from major chickpea growing areas of Kurnool, Anantapur, Prakasam and Kadapa districts of Andhra Pradesh. The pathogen was isolated from infected plants and various morphological and cultural characteristics like size and shape of conidia, size of setae, chlamydospores, colony color, colony diameter, sectoring in the culture, mycelia dry weight and sporulation were studied and a great variability was observed among the isolates.
    Type of Medium: Online Resource
    ISSN: 0976-0571 , 0250-5371
    URL: Article
    DOI: 10.18805/lr.v0iOF.9563
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2016
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Field Evaluation of the Efficacy of Fungicides and Rhizosphere Bacterial Antagonists against Rice Stem Rot Disease
    Sciencedomain International ; 2023
    In:  International Journal of Plant & Soil Science Vol. 35, No. 11 ( 2023-05-04), p. 105-111
    Details
    In: International Journal of Plant & Soil Science, Sciencedomain International, Vol. 35, No. 11 ( 2023-05-04), p. 105-111
    Abstract: Rice stem rot disease in paddy cultivation leads to reduction of yield. Management of this disease is difficult as it is soil borne. In the present study, field trials were conducted during Rabi 2016-17 and early Kharif 2017-18 to assess the effectiveness of twelve fungicides and four rhizosphere bacterial antagonistic isolates (RRB-1 to RRB-4) against rice stem rot disease. Disease incidence was recorded from the date of initial incidence of the disease i.e., once in 15 days from maximum tillering to panicle emergence. Pooled analysis reveals that Hexaconazole recorded lowest per cent disease index (32.22) with highest grain yield and straw yield of 7.43 t/ha and 7.22 t/ha respectively, followed by Difenoconazole with grain yield and straw yield of 7.29 t/ha and 6.88 t/ha respectively. On the other hand, bacterial antagonists using root dipping and foliar spray with RRB-4 showed less PDI of 32.92 with grain yield of 6.97 t/ha and straw yield of 6.61 t/ha. With this study, hexaconazole (0.2%) and bacterial antagonists RRB-4 using root dipping and foliar spray (105 to 106 CFU/ml) can be effectively managed the rice stem rot.
    Type of Medium: Online Resource
    ISSN: 2320-7035
    URL: Article
    DOI: 10.9734/ijpss/2023/v35i112951
    Language: Unknown
    Publisher: Sciencedomain International
    Publication Date: 2023
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Field Evaluation of the Strategies of Integration of Fungicides and Bio Control Agents for Efficient Management of Rice Stem Rot Disease
    Excellent Publishers ; 2018
    In:  International Journal of Current Microbiology and Applied Sciences Vol. 7, No. 07 ( 2018-07-20), p. 3796-3800
    Details
    In: International Journal of Current Microbiology and Applied Sciences, Excellent Publishers, Vol. 7, No. 07 ( 2018-07-20), p. 3796-3800
    Type of Medium: Online Resource
    ISSN: 2319-7692 , 2319-7706
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.20546/ijcmas.2018.707
    DOI: 10.20546/ijcmas
    DOI: 10.20546/ijcmas.2018.707.440
    Language: Unknown
    Publisher: Excellent Publishers
    Publication Date: 2018
    detail.hit.zdb_id: 2697628-6
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...