In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 18, No. 5 ( 2022-5-3), p. e1010488-
Abstract:
Transmitted/founder (T/F) HIV-1 envelope proteins (Envs) from infected individuals that developed neutralization breadth are likely to possess inherent features desirable for vaccine immunogen design. To explore this premise, we conducted an immunization study in rhesus macaques (RM) using T/F Env sequences from two human subjects, one of whom developed potent and broad neutralizing antibodies (Z1800M) while the other developed little to no neutralizing antibody responses (R66M) during HIV-1 infection. Using a DNA/MVA/protein immunization protocol, 10 RM were immunized with each T/F Env. Within each T/F Env group, the protein boosts were administered as either monomeric gp120 or stabilized trimeric gp140 protein. All vaccination regimens elicited high titers of antigen-specific IgG, and two animals that received monomeric Z1800M Env gp120 developed autologous neutralizing activity. Using early Env escape variants isolated from subject Z1800M as guides, the serum neutralizing activity of the two immunized RM was found to be dependent on the gp120 V5 region. Interestingly, the exact same residues of V5 were also targeted by a neutralizing monoclonal antibody (nmAb) isolated from the subject Z1800M early in infection. Glycan profiling and computational modeling of the Z1800M Env gp120 immunogen provided further evidence that the V5 loop is exposed in this T/F Env and was a dominant feature that drove neutralizing antibody targeting during infection and immunization. An expanded B cell clonotype was isolated from one of the neutralization-positive RM and nmAbs corresponding to this group demonstrated V5-dependent neutralization similar to both the RM serum and the human Z1800M nmAb. The results demonstrate that neutralizing antibody responses elicited by the Z1800M T/F Env in RM converged with those in the HIV-1 infected human subject, illustrating the potential of using immunogens based on this or other T/F Envs with well-defined immunogenicity as a starting point to drive breadth.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1010488
DOI:
10.1371/journal.ppat.1010488.g001
DOI:
10.1371/journal.ppat.1010488.g002
DOI:
10.1371/journal.ppat.1010488.g003
DOI:
10.1371/journal.ppat.1010488.g004
DOI:
10.1371/journal.ppat.1010488.g005
DOI:
10.1371/journal.ppat.1010488.g006
DOI:
10.1371/journal.ppat.1010488.g007
DOI:
10.1371/journal.ppat.1010488.g008
DOI:
10.1371/journal.ppat.1010488.g009
DOI:
10.1371/journal.ppat.1010488.g010
DOI:
10.1371/journal.ppat.1010488.g011
DOI:
10.1371/journal.ppat.1010488.g012
DOI:
10.1371/journal.ppat.1010488.g013
DOI:
10.1371/journal.ppat.1010488.g014
DOI:
10.1371/journal.ppat.1010488.g015
DOI:
10.1371/journal.ppat.1010488.s001
DOI:
10.1371/journal.ppat.1010488.s002
DOI:
10.1371/journal.ppat.1010488.s003
DOI:
10.1371/journal.ppat.1010488.s004
DOI:
10.1371/journal.ppat.1010488.s005
DOI:
10.1371/journal.ppat.1010488.s006
DOI:
10.1371/journal.ppat.1010488.s007
DOI:
10.1371/journal.ppat.1010488.s008
DOI:
10.1371/journal.ppat.1010488.s009
DOI:
10.1371/journal.ppat.1010488.s010
DOI:
10.1371/journal.ppat.1010488.s011
DOI:
10.1371/journal.ppat.1010488.s012
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2205412-1
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