In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 7 ( 2023-7-26), p. e0287205-
Abstract:
Rodents have the capacity for spontaneous bladder regeneration and bladder smooth muscle cell (BSMC) migration following a subtotal cystectomy (STC). YAP/WWTR1 and BDNF (Brain-derived neurotrophic factor) play crucial roles in development and regeneration. During partial bladder outlet obstruction (PBO), excessive YAP/WWTR1 signaling and BDNF expression increases BSMC hypertrophy and dysfunction. YAP/WWTR1 and expression of BDNF and CYR61 were examined in models of regeneration and wound repair. Live cell microscopy was utilized in an ex vivo model of STC to visualize cell movement and division. In Sprague-Dawley female rats, STC was performed by resection of the bladder dome sparing the trigone, followed by closure of the bladder. Smooth muscle migration and downstream effects on signaling and expression were also examined after scratch wound of BSMC with inhibitors of YAP and BDNF signaling. Sham, PBO and incision (cystotomy) were comparators for the STC model. Scratch wound in vitro increased SMC migration and expression of BDNF , CTGF and CYR61 in a YAP/WWTR1-dependent manner. Inhibition of YAP/WWTR1 and BDNF signaling reduced scratch-induced migration. BDNF and CYR61 expression was elevated during STC and PBO. STC induces discrete genes associated with endogenous de novo cell regeneration downstream of YAP/WWTR1 activation.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0287205
DOI:
10.1371/journal.pone.0287205.g001
DOI:
10.1371/journal.pone.0287205.g002
DOI:
10.1371/journal.pone.0287205.g003
DOI:
10.1371/journal.pone.0287205.g004
DOI:
10.1371/journal.pone.0287205.g005
DOI:
10.1371/journal.pone.0287205.g006
DOI:
10.1371/journal.pone.0287205.t001
DOI:
10.1371/journal.pone.0287205.s001
DOI:
10.1371/journal.pone.0287205.s002
DOI:
10.1371/journal.pone.0287205.s003
DOI:
10.1371/journal.pone.0287205.s004
DOI:
10.1371/journal.pone.0287205.s005
DOI:
10.1371/journal.pone.0287205.s006
DOI:
10.1371/journal.pone.0287205.s007
DOI:
10.1371/journal.pone.0287205.s008
DOI:
10.1371/journal.pone.0287205.s009
DOI:
10.1371/journal.pone.0287205.r001
DOI:
10.1371/journal.pone.0287205.r002
DOI:
10.1371/journal.pone.0287205.r003
DOI:
10.1371/journal.pone.0287205.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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