In:
The Journal of Immunology, The American Association of Immunologists, Vol. 190, No. 1_Supplement ( 2013-05-01), p. 202.1-202.1
Abstract:
Silver nanomaterials are increasingly being used as antimicrobial agents in medical devices, yet their effects on immunocompetence are unclear. This study investigated modulation of immune cell profiles and cell activation by exposure to silver nanoparticles (AgNP). Mice were injected iv (each day for 2 d) with 66 or 300 µg of either 20 or 110 nm AgNP (or sodium citrate) and sacrificed 1 d later. Flow cytometric analysis revealed that injection of 66 µg of 20 nm AgNP increased Th2 cells and decreased effector memory cells, while exposure to the same dose of larger-sized AgNP had no effect on numbers of CD3+,CD4+, Th1, Th2, Th17 cells, CD4/CD8 ratio, or total memory/naïve/central memory/effector memory cells compared to controls. At 300 µg, 20 nm AgNP significantly decreased the number of naïve CD4+ cells and CD3+CD4- cells (i.e., cytotoxic T cells), while increasing central memory cells, activated central memory cells, effector memory cells, activated effector memory cells, total memory cells, and natural killer cells. A somewhat different cell profile was observed following exposure to the same dose of 110 nm AgNP including an increase in activated CD11c+ cells (macrophages). Taken together, these data indicate that systemic AgNP exposure can modulate the immune system in a dose-dependent manner, with smaller size particles leading to more drastic effects. Overall, these studies have policy implications and demonstrate the need for protecting already vulnerable individuals.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.190.Supp.202.1
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2013
detail.hit.zdb_id:
1475085-5
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