In:
Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 28, No. 3 ( 1980-03), p. 231-237
Abstract:
Lysosomal cathepsin D has been localized with the electron microscope employing an indirect immunohistochemical method using peroxidase labeled, monospecific antibody Fab' subunits. The acid proteinase has been demonstrated within secondary lysosomes of cardiac myocytes and interstitial cells, but not in components of the Golgi apparatus or endoplasmic reticulum. Incubations with a variety of peroxidatic inhibitors suggests that the staining that is observed in secondary lysosomes is attributable to the peroxidase-labeled antibody and not to endogenous oxidation of DAB. The protocol outlined here provides a reproducible method to localize the major lysosomal acid proteinase of the heart at the subcellular level.
Type of Medium:
Online Resource
ISSN:
0022-1554
,
1551-5044
DOI:
10.1177/28.3.6986433
Language:
English
Publisher:
SAGE Publications
Publication Date:
1980
detail.hit.zdb_id:
1421306-0
SSG:
12
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