In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 72, No. 8_Supplement ( 2012-04-15), p. 195-195
Abstract:
High-grade gliomas are some of the most lethal forms of human cancer and new clinical biomarkers and therapeutic targets are highly needed. MicroRNAs (miRNAs), a group of short noncoding RNAs, hold great potential as new biomarkers and targets as they are commonly deregulated in a variety of diseases including gliomas. MicroRNA-21 (miR-21) is the most consistently over-expressed miRNA in several cancers including gliomas and is therefore very promising as a useful clinical biomarker and therapeutic target. To better understand the role of miR-21 in gliomas, paraffin embedded glioma tissue samples from 193 patients with grade I, II, III and IV tumors were analyzed by in situ hybridization (ISH) using LNA-DNA chimeric probes. We found miR-21 expression in tumor cells and tumor-associated blood vessels, whereas no expression was seen in adjacent normal brain parenchyma. Using advanced image analysis we obtained quantitative estimates reflecting the miR-21 expression levels. The miR-21 levels correlated significantly with grade (p = 0.027, rs = 0.161, 95% CI, 0.015 to 0.301) with the highest levels measured in grade IV gliomas. Furthermore, quantitative estimates obtained for the tumor cells alone were significantly associated with poor prognosis in all tumors (p = 0.012) and grade III and IV tumors alone (p = 0.005). This was independent of known clinical parameters (age, grade and sex). In conclusion, we have shown that miR-21 is constricted to tumor cells and tumor blood vessels and that tumor cell miR-21 represent a valuable prognostic biomarker for the disease. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 195. doi:1538-7445.AM2012-195
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2012-195
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2012
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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