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  • 1
    Online Resource
    Online Resource
    Molecular detection of Ehrlichia canis from canine spleen tissue and its association with renal lesions in dogs
    Diva Enterprises Private Limited ; 2019
    In:  Indian Journal of Veterinary Pathology Vol. 43, No. 4 ( 2019), p. 282-
    Details
    In: Indian Journal of Veterinary Pathology, Diva Enterprises Private Limited, Vol. 43, No. 4 ( 2019), p. 282-
    Type of Medium: Online Resource
    ISSN: 0250-4758 , 0973-970X
    URL: Article
    DOI: 10.5958/0973-970X.2019.00055.5
    Language: English
    Publisher: Diva Enterprises Private Limited
    Publication Date: 2019
    detail.hit.zdb_id: 2387540-9
    SSG: 22
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  • 2
    Online Resource
    Online Resource
    Genotyping of HF Crossbred Breeding Bulls for A1/A2 Variant of β-Casein Gene
    Agricultural Research Communication Center ; 2022
    In:  Indian Journal of Animal Research , No. Of ( 2022-1-20)
    Details
    In: Indian Journal of Animal Research, Agricultural Research Communication Center, , No. Of ( 2022-1-20)
    Abstract: Background: The variants of β-casein (CSN 2) gene viz. A1 and A2 are supposed to be related with human health. Hence, to know the status of breeding bulls used for AI services were screened for A1 and A2 variants. Total 47 blood samples of crossbred breeding bulls from Frozen Semen Laboratory, Pune and Aurangabad were used for present work. Methods: The extraction of genomic DNA by phenol-chloroform method were carried out from the blood samples of 47 breeding bulls. Further, DNA based PCR-RFLP method was used to identify the A1, A2 variants. Pure DNA fragments of b-casein (CSN 2) gene were amplified by PCR using suitable pairs of primer to amplify 121 bp and 244 bp products. The primer used to amplify 244 bp was allele specific. The 121 bp fragment then subjected to restriction enzyme digestion. The restriction enzyme digestions resulted into two types of band pattern. The 244 bp fragment was used for validation of A1 and A2 allele of 121 bp. Result: Two types of genotypes i.e. A1A2 and A2A2 were revealed in the experimental population of HF crossbred breeding bulls. The genotypic frequency of A1A2 and A2A2 was estimated 65 and 35 per cent, respectively. The present frequency status of A1 and A2 allele in the breeding bulls may be suggested for planning future breeding strategy in dairy cattle.
    Type of Medium: Online Resource
    ISSN: 0976-0555 , 0367-6722
    URL: Article
    DOI: 10.18805/IJAR.B-4726
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2022
    SSG: 22
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    A REVIEW ON CONCEPT OF KIKKISA (STRIAE GRAVIDARUM)
    World Wide Journals ; 2024
    In:  GLOBAL JOURNAL FOR RESEARCH ANALYSIS
    Details
    In: GLOBAL JOURNAL FOR RESEARCH ANALYSIS, World Wide Journals
    Abstract: Pregnancy is one of the important and beautiful phase in women's life. While experiencing the pregnancy women endures the intense physical and anatomical changes in her body. Kikkisa is one such ailment which occur during the 7th month of pregnancy or 2nd trimester of pregnancy; affecting the skin of the belly, breast region, groin region, and backside as well. According to the Ayurveda kikkisa is skin disorder associated with Garbotpeedana(foetus growth), Kandu( itching) and Vidaha (burning sensation). Is is more commonly correlated with Striae gravidarum where scarlike lesions appears on the breasts, thighs, abdomen, and buttocks. Skin conditions that develop during pregnancy can be dangerous to the woman for a very long time and affect how others perceive her face. So the present study aims to evaluate the etymology of Kikissa(Striae Gravidarum) according to both traditional and modern aspects so as to develop the effective management therapy for the natural maintenance of skin and body.
    Type of Medium: Online Resource
    URL: Journal
    URL: Article
    DOI: 10.36106/gjra
    DOI: 10.36106/gjra/8908150
    Language: English
    Publisher: World Wide Journals
    Publication Date: 2024
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  • 4
    Online Resource
    Online Resource
    ​POU1F1 Gene Polymorphism and its Associations with Body Weight of Osmanabadi Goat
    Agricultural Research Communication Center ; 2021
    In:  Indian Journal of Animal Research , No. Of ( 2021-10-27)
    Details
    In: Indian Journal of Animal Research, Agricultural Research Communication Center, , No. Of ( 2021-10-27)
    Abstract: Background: Osmanabadi is the premium meat type goat breed of Maharashtra, known for its adaptability and reproductive efficiency. Identification of superior germplasm and incorporating them into breeding programme is the need of hour for augmenting productivity of Osmanabadi as well as other indigenous goat breeds. Hence, the present research was aimed to study Pituitary Specific Transcription Factor I (POU1F1) gene polymorphism and its associations with body weight of Osmanabadi goat. Methods: Genomic DNA was extracted from 217 blood samples of randomly selected Osmanabadi goats. The 450 bp fragment of POU1F1 gene encompassing exon-6 and its flanking region (3’UTR) was PCR amplified. The PCR amplicon was subjected to RFLP using AluI and PstI restriction enzymes to identify polymorphism at nucleotide position 174 (T greater than C) in exon 6 and at nucleotide position 110 (T greater than C) in the 3’UTR of POU1F1 gene, respectively. The association of the observed allelic variants / genotypes with body weight of the animals was performed using One-way ANOVA. Result: The AluI PCR-RFLP of 450 bp amplicon of POU1F1 gene in Osmanabadi goats revealed polymorphism with three different genotypes viz., ‘CC’, ‘TT’ and ‘TC’. The observed frequencies for TT, TC and CC genotypes were 0.604, 0.355 and 0.041 respectively. The frequencies of T and C alleles were found to be 0.781 and 0.219, respectively. However, PstI PCR-RFLP revealed monomorphic 3’UTR of POU1F1 gene with single TT genotype. We observed significant differences (P less than 0.01) between various genotypes at AluI exon-6 locus of POU1F1 gene and body weights at birth, 3 and 6 months of Osmanabadi goat. Highest body weight was recorded for TT genotype followed by TC and CC genotypes in all three age groups. Our findings indicate that the AluI PCR-RFLP locus of the exon-6 of POU1F1 gene can be used as a potent molecular marker for selection of superior stock of Osmanabadi goats.
    Type of Medium: Online Resource
    ISSN: 0976-0555 , 0367-6722
    URL: Article
    DOI: 10.18805/IJAR.B-4528
    Language: Unknown
    Publisher: Agricultural Research Communication Center
    Publication Date: 2021
    SSG: 22
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    DataSheet_1.pdf
    Frontiers Media SA ; 2021
    In:  Frontiers in Immunology Vol. 12 ( 2021-6-14)
    Details
    In: Frontiers in Immunology, Frontiers Media SA, Vol. 12 ( 2021-6-14)
    Abstract: The role of sMAdCAM, an important gut immune migratory marker, remains unexplored in COVID-19 pathogenesis considering recent studies positing the gut as a sanctuary site for SARS-CoV-2 persistence. Thus, assimilating profiles of systemic inflammatory mediators with sMAdCAM levels may provide insights into the progression of COVID-19 disease. Also, the role of these markers in governing virus specific immunity following infection remains largely unexplored. A cohort (n = 84) of SARS-C0V-2 infected individuals included a group of in-patients (n = 60) at various stages of disease progression together with convalescent individuals (n = 24) recruited between April and June 2020 from Mumbai, India. Follow-up of 35 in-patients at day 7 post diagnosis was carried out. Th1/Th2/Th17 cytokines along with soluble MAdCAM (sMAdCAM) levels in plasma were measured. Also, anti-viral humoral response as measured by rapid antibody test (IgG, IgM), Chemiluminescent Immunoassay (IgG), and antibodies binding to SARS-CoV-2 proteins were measured by Surface Plasmon Resonance (SPR) from plasma. IL-6 and sMAdCAM levels among in-patients inversely correlated with one another. When expressed as a novel integrated marker—sMIL index (sMAdCAM/IL-6 ratio)—these levels were incrementally and significantly higher in various disease states with convalescents exhibiting the highest values. Importantly, sMAdCAM levels as well as sMIL index (fold change) correlated with peak association response units of receptor binding domain and fold change in binding to spike respectively as measured by SPR. Our results highlight key systemic and gut homing parameters that need to be monitored and investigated further to optimally guide therapeutic and prophylactic interventions for COVID-19.
    Type of Medium: Online Resource
    ISSN: 1664-3224
    URL: Article
    URL: Article
    DOI: 10.3389/fimmu.2021.619906
    DOI: 10.3389/fimmu.2021.619906.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2606827-8
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