In:
ASAIO Journal, Ovid Technologies (Wolters Kluwer Health), Vol. 69, No. 5 ( 2023-05), p. 467-474
Abstract:
The loss of high molecular weight multimers (HMWM) of von Willebrand factor (vWF) in aortic stenosis (AS) and continuous-flow left ventricular assist devices (cf-LVADs) is believed to be associated with high turbulent blood shear. The objective of this study is to understand the degradation mechanism of HMWM in terms of exposure time (kinetic) and flow regime (dynamics) within clinically relevant pathophysiologic conditions. A custom high-shear rotary device capable of creating fully controlled exposure times and flows was used. The system was set so that human platelet-poor plasma flowed through at 1.75 ml/sec, 0.76 ml/sec, or 0.38 ml/sec resulting in the exposure time ( t exp ) of 22, 50, or 100 ms, respectively. The flow was characterized by the Reynolds number (Re). The device was run under laminar (Re = 1,500), transitional (Re = 3,000; Re = 3,500), and turbulent (Re = 4,500) conditions at a given t exp followed by multimer analysis. No degradation was observed at laminar flow at all given t exp . Degradation of HMWM at a given t exp increases with the Re. Re ( p 〈 0.0001) and t exp ( p = 0.0034) are significant factors in the degradation of HMWM. Interaction between Re and t exp , however, is not always significant ( p = 0.73).
Type of Medium:
Online Resource
ISSN:
1058-2916
DOI:
10.1097/MAT.0000000000001848
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2023
detail.hit.zdb_id:
2083312-X
Permalink