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  • 1
    Online Resource
    Online Resource
    Flowering genes in Metrosideros fit a broad herbaceous model encompassing Arabidopsis and Antirrhinum
    Wiley ; 2004
    In:  Physiologia Plantarum Vol. 121, No. 1 ( 2004-05), p. 163-173
    Details
    In: Physiologia Plantarum, Wiley, Vol. 121, No. 1 ( 2004-05), p. 163-173
    Abstract: Molecular studies were conducted on Metrosideros excelsa to determine if the current genetic models for flowering with regard to inflorescence and floral meristem identity genes in annual plants were applicable to a woody perennial. MEL , MESAP1 and METFL1 , the fragments of LEAFY ( LFY ), APETALA1 ( AP1 ) and TERMINAL FLOWER1 ( TFL1 ) equivalents, respectively, were isolated from M. excelsa . Temporal expression patterns showed that MEL and MESAP1 exhibited a bimodal pattern of expression. Expression exhibited during early floral initiation in autumn was followed by down‐regulation during winter, and up‐regulation in spring as floral organogenesis occurred. Spatial expression patterns of MEL showed that it had greater similarity to FLORICAULA ( FLO ) than to LFY , whereas MESAP1 was more similar to AP1 than SQUAMOSA . The interaction between MEL and METFL1 was more similar to the interaction between FLO and CENTRORADIALIS than that between LFY and TFL1 . Consequently, the three genes from M. excelsa fit a broader herbaceous model encompassing Antirrhinum as well as Arabidopsis , but with differences, such as the bimodal pattern of expression seen with MEL and MESAP1 . In mid‐winter, at the time when both MEL and MESAP1 were down‐regulated, GA 1 was below the level of detection in M. excelsa buds. Even though application of gibberellin inhibits flowering in members of the Myrtaceae, MEL was responsive to gibberellin with expression in juvenile plants up‐regulated by GA 3 . However, MESAP1 was not up‐regulated indicating that meristem competence was also probably required to promote flowering in M. excelsa .
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    URL: Article
    DOI: 10.1111/ppl.2004.121.issue-1
    DOI: 10.1111/j.0031-9317.2004.00304.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2004
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    detail.hit.zdb_id: 2020837-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    185 Effects of Prohexadione-Ca on Gibberellin Levels in Young Apple Shoots
    American Society for Horticultural Science ; 2000
    In:  HortScience Vol. 35, No. 3 ( 2000-06), p. 422D-422
    Details
    In: HortScience, American Society for Horticultural Science, Vol. 35, No. 3 ( 2000-06), p. 422D-422
    Abstract: Prohexadione-Ca (BAS 125 W) is a new growth retardant for the inhibition of excessive vegetative growth in apple and other plant species. From work with enzyme preparations, it is known that prohexadione-Ca mimics 2-oxoglutaric acid, the co-substrate of dioxygenases, which catalyze late steps in gibberellin (GA) biosynthesis. As a result, the formation of growth-active GAs is reduced. In order to have a better understanding of its effects in intact plants, we have analyzed the GA status of treated and untreated apple plantlets. In a typical experiment, the following results were obtained: Plants (cv. Jonagold on M9 at 19 cm of new shoot growth) were sprayed until run-off with an aqueous preparation containing 25 ppm of active ingredient. After 22 days of cultivation under greenhouse conditions, total new shoot growth of the controls and the treated plants was 55 cm and 44 cm, respectively. In the apical part of this material the following GAs (roughly ordered in biosynthetic sequence) were detected at the following levels (control/treated in microgram per kilogram dry weight): GA 19 (31/62), GA29 (24/36), GA20 (11/20), GA1 (4/3), and GA 8 (8/3). These results clearly demonstrate that prohexadione-Ca blocks primarily the hydroxylation of GA 20 into GA 1 . This leads to reduced levels of the highly active GA 1 and of GA 8 , its inactive metabolite, whereas GA 20 and the other inactive precursors accumulate. The data support older observations obtained in vitro, which indicate that GA 20 3β-hydroxylase and related dioxygenases are the primary targets of prohexadione-Ca and similar compounds.
    Type of Medium: Online Resource
    ISSN: 0018-5345 , 2327-9834
    URL: Article
    DOI: 10.21273/HORTSCI.35.3.422D
    Language: Unknown
    Publisher: American Society for Horticultural Science
    Publication Date: 2000
    detail.hit.zdb_id: 2040198-X
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  • 3
    Online Resource
    Online Resource
    Outreach within the Bristol ChemLabS CETL (Centre for Excellence in Teaching and Learning)
    Canadian Center of Science and Education ; 2013
    In:  Higher Education Studies Vol. 3, No. 1 ( 2013-01-25)
    Details
    In: Higher Education Studies, Canadian Center of Science and Education, Vol. 3, No. 1 ( 2013-01-25)
    Type of Medium: Online Resource
    ISSN: 1925-475X , 1925-4741
    URL: Article
    DOI: 10.5539/hes.v3n1p39
    Language: Unknown
    Publisher: Canadian Center of Science and Education
    Publication Date: 2013
    detail.hit.zdb_id: 2658154-1
    SSG: 5,3
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  • 4
    Online Resource
    Online Resource
    Gibberellin Biosynthesis in Maize. Metabolic Studies with GA15, GA24, GA25, GA7, and 2,3-Dehydro-GA9  
    Oxford University Press (OUP) ; 1999
    In:  Plant Physiology Vol. 121, No. 3 ( 1999-11-01), p. 1037-1045
    Details
    In: Plant Physiology, Oxford University Press (OUP), Vol. 121, No. 3 ( 1999-11-01), p. 1037-1045
    Abstract: [17-14C]-Labeled GA15, GA24, GA25, GA7, and 2,3-dehydro-GA9 were separately injected into normal,dwarf-1 (d1), and dwarf-5 (d5) seedlings of maize (Zea mays L.). Purified radioactive metabolites from the plant tissues were identified by full-scan gas chromatography-mass spectrometry and Kovats retention index data. The metabolites from GA15 were GA44, GA19, GA20, GA113, and GA15-15,16-ene (artifact?). GA24 was metabolized to GA19, GA20, and GA17. The metabolites from GA25 were GA17, GA25 16α,17-H2-17-OH, and HO-GA25 (hydroxyl position not determined). GA7was metabolized to GA30, GA3, isoGA3 (artifact?), and trace amounts of GA7-diene-diacid (artifact?). 2,3-Dehydro-GA9was metabolized to GA5, GA7 (trace amounts), 2,3-dehydro-GA10 (artifact?), GA31, and GA62. Our results provide additional in vivo evidence of a metabolic grid in maize (i.e. pathway convergence). The grid connects members of a putative, non-early 3,13-hydroxylation branch pathway to the corresponding members of the previously documented early 13-hydroxylation branch pathway. The inability to detect the sequence GA12 → GA15 → GA24 → GA9 indicates that the non-early 3,13-hydroxylation pathway probably plays a minor role in the origin of bioactive gibberellins in maize.
    Type of Medium: Online Resource
    ISSN: 1532-2548 , 0032-0889
    URL: Article
    DOI: 10.1104/pp.121.3.1037
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1999
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Ethnic differences in the indirect effects of the COVID-19 pandemic on clinical monitoring and hospitalisations for non-COVID conditions in England: a population-based, observational cohort study using the OpenSAFELY platform
    Elsevier BV ; 2023
    In:  eClinicalMedicine Vol. 61 ( 2023-07), p. 102077-
    Details
    In: eClinicalMedicine, Elsevier BV, Vol. 61 ( 2023-07), p. 102077-
    Type of Medium: Online Resource
    ISSN: 2589-5370
    URL: Article
    DOI: 10.1016/j.eclinm.2023.102077
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2946413-4
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  • 6
    Online Resource
    Online Resource
    Gaming science: the “Gamification” of scientific thinking
    Frontiers Media SA ; 2013
    In:  Frontiers in Psychology Vol. 4 ( 2013)
    Details
    In: Frontiers in Psychology, Frontiers Media SA, Vol. 4 ( 2013)
    Type of Medium: Online Resource
    ISSN: 1664-1078
    URL: Article
    DOI: 10.3389/fpsyg.2013.00607
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2013
    detail.hit.zdb_id: 2563826-9
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  • 7
    Online Resource
    Online Resource
    Improving the assessment and management of obesity in UK children and adolescents: the PROMISE research programme including a RCT
    National Institute for Health and Care Research ; 2020
    In:  Programme Grants for Applied Research Vol. 8, No. 3 ( 2020-3), p. 1-264
    Details
    In: Programme Grants for Applied Research, National Institute for Health and Care Research, Vol. 8, No. 3 ( 2020-3), p. 1-264
    Abstract: Five linked studies were undertaken to inform identified evidence gaps in the childhood obesity pathway. Objectives (1) To scope the impact of the National Child Measurement Programme (NCMP) (study A). (2) To develop a brief evidence-based electronic assessment and management tool (study B). (3) To develop evidence-based algorithms for identifying the risk of obesity comorbidities (study B). (4) To conduct an efficacy trial of the Healthy Eating and Lifestyle Programme (HELP) (study C). (5) To improve the prescribing of anti-obesity drugs in UK adolescents (study D). (6) To investigate the safety, outcomes and predictors of outcome of adolescent bariatric surgery in the UK (study E). Methods Five substudies – (1) a parental survey before and after feedback from the National Childhood Measurement Programme, (2) risk algorithm development and piloting of a new primary care management tool, (3) a randomised controlled trial of the Healthy Eating and Lifestyle Programme, (4) quantitative and qualitative studies of anti-obesity drug treatment in adolescents and (5) a prospective clinical audit and cost-effectiveness evaluation of adolescent bariatric surgery in one centre. Results Study A – before the National Childhood Measurement Programme feedback, three-quarters of parents of overweight and obese children did not recognise their child to be overweight. Eighty-seven per cent of parents found the National Childhood Measurement Programme feedback to be helpful. Feedback had positive effects on parental knowledge, perceptions and intentions. Study B – risk estimation models for cardiovascular and psychosocial comorbidities of obesity require further development. An online consultation tool for primary care practitioners is acceptable and feasible. Study C – the Healthy Eating and Lifestyle Programme, when delivered in the community by graduate mental health workers, showed no significant effect on body mass index at 6 months (primary outcome) when compared with enhanced usual care. Study D – anti-obesity drugs appear efficacious in meta-analysis, and their use has expanded rapidly in the last decade. However, the majority of prescriptions are rapidly discontinued after 1–3 months of treatment. Few young people described positive experiences of anti-obesity drugs. Prescribing was rarely compliant with the National Institute for Health and Care Excellence guidance. Study E – bariatric surgery appears safe, effective and highly cost-effective in adolescents in the NHS. Future work and limitations Work is needed to evaluate behaviour and body mass index change in the National Childhood Measurement Programme more accurately and improve primary care professionals’ understanding of the National Childhood Measurement Programme feedback, update and further evaluate the Computer-Assisted Treatment of CHildren (CATCH) tool, investigate delivery of weight management interventions to young people from deprived backgrounds and those with significant psychological distress and obtain longer-term data on anti-obesity drug use and bariatric surgery outcomes in adolescence. Trial registration Current Controlled Trials ISRCTN99840111. Funding This project was funded by the National Institute for Health Research (NIHR) Programme Grants for Applied Research programme and will be published in full in Programme Grants for Applied Research ; Vol. 8, No. 3. See the NIHR Journals Library website for further project information.
    Type of Medium: Online Resource
    ISSN: 2050-4322 , 2050-4330
    URL: Article
    DOI: 10.3310/pgfar08030
    Language: English
    Publisher: National Institute for Health and Care Research
    Publication Date: 2020
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