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  • 1
    Online Resource
    Online Resource
    Electrochemical Impedance Studies on Single and Multi-Walled Carbon Nanotubes—Polymer Nanocomposites for Biosensors Development
    American Scientific Publishers ; 2015
    In:  Journal of Nanoscience and Nanotechnology Vol. 15, No. 5 ( 2015-05-01), p. 3385-3393
    Details
    In: Journal of Nanoscience and Nanotechnology, American Scientific Publishers, Vol. 15, No. 5 ( 2015-05-01), p. 3385-3393
    Type of Medium: Online Resource
    ISSN: 1533-4880 , 1533-4899
    URL: Article
    DOI: 10.1166/jnn.2015.10208
    Language: English
    Publisher: American Scientific Publishers
    Publication Date: 2015
    SSG: 11
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  • 2
    Online Resource
    Online Resource
    Electrochemical sensors based on carbon nanomaterials for acetaminophen detection: A review
    Elsevier BV ; 2015
    In:  Analytica Chimica Acta Vol. 886 ( 2015-07), p. 16-28
    Details
    In: Analytica Chimica Acta, Elsevier BV, Vol. 886 ( 2015-07), p. 16-28
    Type of Medium: Online Resource
    ISSN: 0003-2670
    URL: Article
    DOI: 10.1016/j.aca.2015.05.044
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    detail.hit.zdb_id: 52-8
    detail.hit.zdb_id: 1483436-4
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  • 3
    Online Resource
    Online Resource
    Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection
    MDPI AG ; 2021
    In:  Molecules Vol. 26, No. 11 ( 2021-05-27), p. 3200-
    Details
    In: Molecules, MDPI AG, Vol. 26, No. 11 ( 2021-05-27), p. 3200-
    Abstract: Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules26113200
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2008644-1
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  • 4
    Online Resource
    Online Resource
    Poly‐(pyrrole‐3‐carboxylic acid) Based Nanostructured Platform for the Detection of Carcinoembryonic Antigen
    Wiley ; 2018
    In:  Electroanalysis Vol. 30, No. 6 ( 2018-06), p. 1100-1106
    Details
    In: Electroanalysis, Wiley, Vol. 30, No. 6 ( 2018-06), p. 1100-1106
    Abstract: A nanostructured platform based on pyrrole 3‐carboxylic acid was developed and it was used for the detection of carcinoembryonic antigen, a biomarker for gastro‐intestinal tract cancers. The conductive polymer offers possibilities for functionalization due to the presence of the carboxylic group. The platform was tailored by polymerizing pyrrole 3‐carboxylic acid at the surface of a graphite‐based screen‐printed electrode by using multiple pulse amperometry and cyclic voltammetry. The carboxylic groups were activated, facilitating the formation of amide covalent bonds with the terminal amine groups from the antibody. Each step's optimization was tracked by performing electrochemical impedance spectroscopy. The immunosensor was successfully applied for the detection of carcinoembryonic antigen in synthetic samples. By comparing pyrrole 3‐carboxylic acid with pyrrole, significantly greater increases of the charge transfer resistance were achieved by using the functionalized monomer for the immobilization step. The binding of the anti‐CEA was reproducible and directly proportional with its concentration. The increase of the charge transfer resistance after the incubation with carcinoembryonic antigen was linearly correlated with the concentration of the sample and the LOD was 33.33 pg mL −1 . A simple nanostructured platform for the label‐free detection of a carcinogenic biomarker molecule was developed, thus holding great opportunities for clinical diagnostics and other biosensor applications.
    Type of Medium: Online Resource
    ISSN: 1040-0397 , 1521-4109
    URL: Issue
    URL: Article
    DOI: 10.1002/elan.v30.6
    DOI: 10.1002/elan.201700803
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1483564-2
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  • 5
    Online Resource
    Online Resource
    Exploring the Research Progress about the Applications of Cyclodextrins and Nanomaterials in Electroanalysis
    Wiley ; 2023
    In:  Electroanalysis Vol. 35, No. 1 ( 2023-01)
    Details
    In: Electroanalysis, Wiley, Vol. 35, No. 1 ( 2023-01)
    Abstract: Cyclodextrins and nanomaterials are widely used in the achievement of powerful platforms in supramolecular chemistry and nanotechnology. The relatively hydrophobic internal cavity of the CDs selectively retains molecules having the proper geometry, while the hydrophilic exterior allows CDs to improve the dispersibility and molecular recognition. The nanomaterials provide higher surface area, good conductivity, and electrocatalytic effect. The use of nanomaterials and CDs in electrochemical sensors’ design allows the development of a large variety of devices, explaining the increasing number of papers in the last years that are discussed in this review.
    Type of Medium: Online Resource
    ISSN: 1040-0397 , 1521-4109
    URL: Issue
    URL: Article
    DOI: 10.1002/elan.v35.1
    DOI: 10.1002/elan.202200014
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1483564-2
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  • 6
    Online Resource
    Online Resource
    Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study
    MDPI AG ; 2021
    In:  Journal of Personalized Medicine Vol. 11, No. 2 ( 2021-02-19), p. 147-
    Details
    In: Journal of Personalized Medicine, MDPI AG, Vol. 11, No. 2 ( 2021-02-19), p. 147-
    Abstract: Methylene blue (MB) is both a dye and a medicine known and used for a long time including as lymphatic tracer in melanoma and breast cancer for revealing sentinel lymph nodes. Proflavine (PRO) is an acriflavine dye, used as bacteriostatic disinfectant against many gram-positive bacteria that was also successfully applied to evaluate morphopathological changes in tissues. This study was performed on a group of twenty-eight Wistar rats and had as its main objective the in vivo evaluation of the use of MB and PRO as perforator tracers. The two dyes proved to be effective functional perforasome tracers with medium inflammatory infiltrate in the skin of the island perforator flap which heals perfectly at 14 days with complete absence of the inflammatory reaction. At the same injected amount, PRO seems to determine a greater inflammatory reaction compared with MB, but in smaller concentration, the inflammatory response is absent in the case of PRO. In conclusion, both substances tested within this in vivo study are good functional perforasome tracers, but PRO has the advantage of the absence of inflammatory reaction when using lower concentrations, while preserving unalerted its efficiency as tracer.
    Type of Medium: Online Resource
    ISSN: 2075-4426
    URL: Article
    DOI: 10.3390/jpm11020147
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2662248-8
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  • 7
    Online Resource
    Online Resource
    Saliva, a Magic Biofluid Available for Multilevel Assessment and a Mirror of General Health—A Systematic Review
    MDPI AG ; 2019
    In:  Biosensors Vol. 9, No. 1 ( 2019-02-14), p. 27-
    Details
    In: Biosensors, MDPI AG, Vol. 9, No. 1 ( 2019-02-14), p. 27-
    Abstract: Background: Saliva has been recently proposed as an alternative to classic biofluid analyses due to both availability and reliability regarding the evaluation of various biomarkers. Biosensors have been designed for the assessment of a wide spectrum of compounds, aiding in the screening, diagnosis, and monitoring of pathologies and treatment efficiency. This literature review aims to present the development in the biosensors research and their utility using salivary assessment. Methods: a comprehensive literature search has been conducted in the PubMed database, using the keywords “saliva” and “sensor”. A two-step paper selection algorithm was devised and applied. Results: The 49 papers selected for the present review focused on assessing the salivary biomarkers used in general diseases, oral pathologies, and pharmacology. The biosensors proved to be reliable tools for measuring the salivary levels of biochemical metabolic compounds such as glucose, proteinases and proteins, heavy metals and various chemical compounds, microorganisms, oncology markers, drugs, and neurotransmitters. Conclusions: Saliva is a biofluid with a significant clinical applicability for the evaluation and monitoring of a patient’s general health. Biosensors designed for assessing a wide range of salivary biomarkers are emerging as promising diagnostic or screening tools for improving the patients’ quality of life.
    Type of Medium: Online Resource
    ISSN: 2079-6374
    URL: Article
    DOI: 10.3390/bios9010027
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2662125-3
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  • 8
    Online Resource
    Online Resource
    Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin
    MDPI AG ; 2019
    In:  Biosensors Vol. 9, No. 1 ( 2019-02-27), p. 31-
    Details
    In: Biosensors, MDPI AG, Vol. 9, No. 1 ( 2019-02-27), p. 31-
    Abstract: In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro‒polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor was successfully tested in the presence of some other cephalosporins and of other pharmaceutical compounds. The developed method was successfully applied to the detection of cefalexin from real environmental and pharmaceutical samples.
    Type of Medium: Online Resource
    ISSN: 2079-6374
    URL: Article
    DOI: 10.3390/bios9010031
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2662125-3
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  • 9
    Online Resource
    Online Resource
    Wearable Sensors for the Detection of Biomarkers for Wound Infection
    MDPI AG ; 2021
    In:  Biosensors Vol. 12, No. 1 ( 2021-12-21), p. 1-
    Details
    In: Biosensors, MDPI AG, Vol. 12, No. 1 ( 2021-12-21), p. 1-
    Abstract: Infection represents a major complication that can affect wound healing in any type of wound, especially in chronic ones. There are currently certain limitations to the methods that are used for establishing a clinical diagnosis of wound infection. Thus, new, rapid and easy-to-use strategies for wound infection diagnosis need to be developed. To this aim, wearable sensors for infection diagnosis have been recently developed. These sensors are incorporated into the wound dressings that are used to treat and protect the wound, and are able to detect certain biomarkers that can be correlated with the presence of wound infection. Among these biomarkers, the most commonly used ones are pH and uric acid, but a plethora of others (lactic acid, oxygenation, inflammatory mediators, bacteria metabolites or bacteria) have also been detected using wearable sensors. In this work, an overview of the main types of wearable sensors for wound infection detection will be provided. These sensors will be divided into electrochemical, colorimetric and fluorimetric sensors and the examples will be presented and discussed comparatively.
    Type of Medium: Online Resource
    ISSN: 2079-6374
    URL: Article
    DOI: 10.3390/bios12010001
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2662125-3
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  • 10
    Online Resource
    Online Resource
    Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials
    MDPI AG ; 2022
    In:  Biosensors Vol. 12, No. 8 ( 2022-08-13), p. 638-
    Details
    In: Biosensors, MDPI AG, Vol. 12, No. 8 ( 2022-08-13), p. 638-
    Abstract: The rapid diagnosis of Pseudomonas aeruginosa infection is very important because this bacterium is one of the main sources of healthcare-associated infections. Pseudomonas quinolone signal (PQS) is a specific molecule for quorum sensing (QS) in P. aeruginosa, a form of cell-to-cell bacterial communication and its levels can allow the determination of the bacterial population. In this study, the development of the first electrochemical detection of PQS using screen-printed electrodes modified with carbon nanotubes (CNT-SPE) is reported. The electrochemical fingerprint of PQS was determined using different electrode materials and screen-printed electrodes modified with different nanomaterials. The optimization of the method in terms of electrolyte, pH, and electrochemical technique was achieved. The quantification of PQS was performed using one of the anodic peaks in the electrochemical fingerprint of the PQS on the CNT-SPE. The sensor exhibited a linear range from 0.1 to 15 µM, with a limit of detection of 50 nM. The sensor allowed the selective detection of PQS, with low interference from other QS molecules. The sensor was successfully applied to analysis of real samples (spiked urine and human serum samples, spiked microbiological growth media, and microbiological cultures).
    Type of Medium: Online Resource
    ISSN: 2079-6374
    URL: Article
    DOI: 10.3390/bios12080638
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662125-3
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