In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 12 ( 2021-3-4)
Abstract:
Isolation of nuclei tagged in specific cell types (INTACT) is a method developed to isolate cell-type-specific nuclei that are tagged through in vivo biotin labeling of a nuclear targeting fusion (NTF) protein. In our work, INTACT was used to capture nuclei of meiocytes and to generate a meiotic transcriptome in Arabidopsis . Using the promoter of AtDMC1 recombinase to label meiotic nuclei, we generated transgenic plants carrying AtDMC1:NTF along with biotin ligase enzyme ( BirA ) under the constitutive ACTIN2 ( ACT2 ) promoter. AtDMC1 -driven expression of biotin-labeled NTF allowed us to collect nuclei of meiocytes by streptavidin-coated magnetic beads. The nuclear meiotic transcriptome was obtained by RNA-seq using low-quantity input RNA. Transcripts grouped into different categories according to their expression levels were investigated by gene ontology enrichment analysis (GOEA). The most enriched GO term “DNA demethylation” in mid/high-expression classes suggests that this biological process is particularly relevant to meiosis onset. The majority of genes with established roles in meiosis were distributed in the classes of mid/high and high expression. Meiotic transcriptome was compared with public available transcriptomes from other tissues in Arabidopsis. Bioinformatics analysis by expression network identified a core of more than 1,500 genes related to meiosis landmarks.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2021.638051
DOI:
10.3389/fpls.2021.638051.s001
DOI:
10.3389/fpls.2021.638051.s002
DOI:
10.3389/fpls.2021.638051.s003
DOI:
10.3389/fpls.2021.638051.s004
DOI:
10.3389/fpls.2021.638051.s005
DOI:
10.3389/fpls.2021.638051.s006
DOI:
10.3389/fpls.2021.638051.s007
DOI:
10.3389/fpls.2021.638051.s008
DOI:
10.3389/fpls.2021.638051.s009
DOI:
10.3389/fpls.2021.638051.s010
DOI:
10.3389/fpls.2021.638051.s011
DOI:
10.3389/fpls.2021.638051.s012
DOI:
10.3389/fpls.2021.638051.s013
DOI:
10.3389/fpls.2021.638051.s014
DOI:
10.3389/fpls.2021.638051.s015
DOI:
10.3389/fpls.2021.638051.s016
DOI:
10.3389/fpls.2021.638051.s017
DOI:
10.3389/fpls.2021.638051.s018
DOI:
10.3389/fpls.2021.638051.s019
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
Permalink