In:
Development, The Company of Biologists, ( 2016-01-01)
Kurzfassung:
The sea urchin larval skeleton offers a simple model for developmental pattern formation. The calcium carbonate skeleton is secreted by primary mesenchyme cells (PMCs) in response to largely unknown patterning cues expressed by the ectoderm. To discover novel ectodermal cues, we performed an unbiased RNA-seq-based screen and functionally tested candidates; we thereby identified several novel skeletal patterning cues. Among these, we show that SLC26a2/7 is a ventrally expressed sulfate transporter which promotes a ventral accumulation of sulfated proteoglycans that is required for ventral PMC positioning and skeletal patterning. We show that the effects of SLC perturbation are mimicked by manipulation of either external sulfate levels or proteoglycan sulfation. These results identify novel skeletal patterning genes and demonstrate that ventral proteoglycan sulfation serves as a positional cue for the sea urchin skeletal patterning.
Materialart:
Online-Ressource
ISSN:
1477-9129
,
0950-1991
Sprache:
Englisch
Verlag:
The Company of Biologists
Publikationsdatum:
2016
ZDB Id:
2007916-3
SSG:
12
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