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  • 1
    In: International Journal of Biological Macromolecules, Elsevier BV, Vol. 181 ( 2021-06), p. 136-149
    Type of Medium: Online Resource
    ISSN: 0141-8130
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 1483284-7
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  • 2
    In: New Disease Reports, Wiley, Vol. 43, No. 2 ( 2021-04)
    Type of Medium: Online Resource
    ISSN: 2044-0588 , 2044-0588
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2160485-X
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  • 3
    Online Resource
    Online Resource
    Scientific Societies ; 2001
    In:  Phytopathology® Vol. 91, No. 3 ( 2001-03), p. 282-292
    In: Phytopathology®, Scientific Societies, Vol. 91, No. 3 ( 2001-03), p. 282-292
    Abstract: During the 1995 wet season, harvested rice seed was collected from farmers' fields at different locations in Iloilo, Philippines. Bacterial isolations from crushed seed yielded 428 isolates. The isolates were characterized by BOX-polymerase chain reaction fingerprinting of total genomic DNA and represented 151 fingerprint types (FPT). Most FPTs were found on a single occasion, although matching fingerprints for isolates from different samples also were found. Identifications were made by cellular fatty acid methyl ester analysis and additional use of Biolog GN/GP MicroPlates and API 20E/50CHE systems. The predominant bacteria were Enterobacteriaceae (25%), Bacillus spp. (22%), and Pseu-domonas spp. (14%). Other bacteria regularly present were identified as Xanthomonas spp., Cellulomonas flavigena, and Clavibacter michiganense. Of the total number of isolated bacteria, 4% exhibited in vitro antifungal activity against Rhizoctonia solani or Pyricularia grisea. Two percent of isolates were pathogens identified as Burkholderia glumae and Burkholderia gladioli. Five percent of isolates induced sheath necrosis on only 50 to 90% of inoculated plants and were related to Bacillus pumilus, Paenibacillus spp., Pseudomonas spp., and Pantoea spp.
    Type of Medium: Online Resource
    ISSN: 0031-949X , 1943-7684
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2001
    detail.hit.zdb_id: 2037027-1
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  • 4
    In: Phytopathology®, Scientific Societies, Vol. 104, No. 5 ( 2014-05), p. 520-531
    Abstract: Bacterial leaf streak (BLS) caused by Xanthomonas oryzae pv. oryzicola was first reported in Africa in the 1980s. Recently, a substantial reemergence of this disease was observed in West Africa. Samples were collected at various sites in five and three different rice-growing regions of Burkina Faso and Mali, respectively. Sixty-seven X. oryzae pv. oryzicola strains were isolated from cultivated and wild rice varieties and from weeds showing BLS symptoms. X. oryzae pv. oryzicola strains were evaluated for virulence on rice and showed high variation in lesion length on a susceptible cultivar. X. oryzae pv. oryzicola strains were further characterized by multilocus sequence analysis (MLSA) using six housekeeping genes. Inferred dendrograms clearly indicated different groups among X. oryzae pv. oryzicola strains. Restriction fragment length polymorphism analysis using the transcriptional activator like effector avrXa7 as probe resulted in the identification of 18 haplotypes. Polymerase chain reaction-based analyses of two conserved type III effector (T3E) genes (xopAJ and xopW) differentiated the strains into distinct groups, with xopAJ not detected in most African X. oryzae pv. oryzicola strains. XopAJ functionality was confirmed by leaf infiltration on ‘Kitaake’ rice Rxo1 lines. Sequence analysis of xopW revealed four groups among X. oryzae pv. oryzicola strains. Distribution of 43 T3E genes shows variation in a subset of X. oryzae pv. oryzicola strains. Together, our results show that African X. oryzae pv. oryzicola strains are diverse and rapidly evolving, with a group endemic to Africa and another one that may have evolved from an Asian strain.
    Type of Medium: Online Resource
    ISSN: 0031-949X , 1943-7684
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2014
    detail.hit.zdb_id: 2037027-1
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  • 5
    Online Resource
    Online Resource
    Wiley ; 2013
    In:  Plant Pathology Vol. 62, No. 5 ( 2013-10), p. 1123-1131
    In: Plant Pathology, Wiley, Vol. 62, No. 5 ( 2013-10), p. 1123-1131
    Abstract: Polyphasic analysis exposed important heterogeneity between bacterial strains catalogued as X anthomonas arboricola pv. fragariae ( X af) from different culture collections. Two draft whole‐genome sequences revealed pathogenicity related genes of the type‐three secretion system in strain LMG 19146, whereas none were found in the X af pathotype strain LMG 19145. Also, considerable sequence divergence was observed in the phylogenetic marker genes gyr B , rpo D , dna K and fyu A . Further study of 16 X af culture‐collection strains showed that co‐classification is not justified. Partial 16S rRNA gene and gyr B sequencing demonstrated that 12 strains belonged to X . arboricola , but that they did not form one homogeneous group within the species. The four remaining strains were identified as X anthomonas fragariae and X anthomonas sp. All sequence‐based identifications were confirmed by MALDI‐TOF MS fingerprinting. Also, the pathogenicity genes hrc Q and avr B s2 were detected in only three of the 12 analysed X .  arboricola strains. The X . arboricola and X anthomonas sp. strains showed pectolytic activity, and upon inoculation in strawberry none of the strains reproduced the leaf blight symptoms reported for X af. This study demonstrates that (i) no clear criteria exist for the identification of strains as X af, (ii) the name X af is currently used for a genetically diverse assortment of strains, and (iii) the species X . arboricola holds many undetermined plant‐associated bacteria besides the described pathovars.
    Type of Medium: Online Resource
    ISSN: 0032-0862 , 1365-3059
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2020845-5
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  • 6
    Online Resource
    Online Resource
    Wiley ; 1999
    In:  Journal of Animal Physiology and Animal Nutrition Vol. 82, No. 5 ( 1999-12), p. 217-226
    In: Journal of Animal Physiology and Animal Nutrition, Wiley, Vol. 82, No. 5 ( 1999-12), p. 217-226
    Type of Medium: Online Resource
    ISSN: 0931-2439 , 1439-0396
    Language: English
    Publisher: Wiley
    Publication Date: 1999
    detail.hit.zdb_id: 2020405-X
    SSG: 12
    SSG: 21
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  • 7
    In: Plant Pathology, Wiley, Vol. 66, No. 7 ( 2017-09), p. 1094-1102
    Abstract: Molecular diagnostics of plant pathogens are crucial to prevent disease spread and to enhance food quality and security. A comparative genomics approach using genomes of different Xanthomonas species and pathovars was applied to identify highly specific targets in the genome of Xanthomonas fragariae , the causal agent of angular leaf spot of strawberry, listed under quarantine regulations in Europe. A reliable and sensitive loop‐mediated isothermal amplification ( LAMP ) assay was designed using a unique marker, providing a highly specific and rapid detection technique, convenient for on‐site detection. Specificity of the designed assay was tested on 37 strains from a culture collection of X. fragariae , 82 strains of other Xanthomonas species and pathovars and 11 strains of other bacterial genera isolated from strawberry leaves. A detection limit of 10 2  fg was achieved, approximating to 20 genome copies per reaction. When performing analyses with crude plant material, a consistent lower detection efficiency of 10 2 CFU   mL −1 was achieved. The LAMP assay designed in this study was adapted to work on crude plant material without any prior extensive extraction steps or incubation period; moreover, it does not require advanced analytical knowledge or a fully equipped laboratory. Results were produced within 7–20 min, depending on the pathogen concentration, thus providing a high‐throughput and user‐friendly method for detection and screening of plant material in support of quarantine regulations.
    Type of Medium: Online Resource
    ISSN: 0032-0862 , 1365-3059
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2020845-5
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  • 8
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 1998
    In:  BSAP Occasional Publication Vol. 22 ( 1998), p. 300-302
    In: BSAP Occasional Publication, Cambridge University Press (CUP), Vol. 22 ( 1998), p. 300-302
    Abstract: In the Dutch protein evaluation system (Tamminga et al ., 1994) microbial protein synthesized in the rumen is estimated from the fermentable organic-matter (FOM) content. The reference method to determine FOM is by in situ incubations of food in the rumen of fistulated animals. However, this is a cumbersome, time-consuming and expensive procedure. In practice, FOM is estimated from the digestible organic-matter content (DOMD) corrected for crude fat (Cfat), rumen escape protein (EP) and starch (ES) and the fermentation products (FP), using tabular values. Apart from being indirect, this approach takes little or no account of the variability within foods. Although an in vitro method based on rumen fluid may give a good prediction of in situ FOM for the majority of the foodstuffs (Cone et al ., 1994), an enzymatic method is preferable for practical reasons. From the organic-matter (OM) degradation by a mixture of amylase and other carbohydrate degrading enzymes at 39°C during 0, 2, 6 and 24 h, Cone et al . (1996) obtained a moderate correlation with in situ FOM for 29 grass and grass silage samples ( R 2 = 0.88; residual s.d. = 37 g/kg dry matter (DM)) and 80 samples of concentrates, maize and clover ( R 2 = 0.77; residual s.d. = 42 g/kg DM). In this study a modified version of the enzymatic method of de Boever et al . (1994), normally used to predict the in vivo OM digestibility and energy value of concentrates, was tested for its reliability to predict in situ FOM of compound foods and forages.
    Type of Medium: Online Resource
    ISSN: 0263-967X , 2053-5953
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 1998
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  • 9
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 1995
    In:  Animal Science Vol. 61, No. 1 ( 1995-08), p. 19-23
    In: Animal Science, Cambridge University Press (CUP), Vol. 61, No. 1 ( 1995-08), p. 19-23
    Abstract: Forty-eight culled dairy cows were assigned to one of two treatment groups to investigate the effect of a β-adrenergic agonist in mature cattle. They were finished during a 63-day period on a concentrate diet, containing 0 or 4 mg/kg cimaterol. No food was given in the 20 h prior to slaughter. Cimaterol had no effect on food intake, but significantly improved food conversion efficiency and increased live-weight gain, carcass weight and dressing proportion (P 〈 0·05). Cimaterol induced muscle hypertrophy as evidenced by an improved EUROP carcass classification and a higher longissimus thoracis (LT) cross-sectional area (P 〈 0·001). EUROP fat score and fat content in the carcass and the LT were lowered (P 〈 0·05). Cimaterol resulted in brighter meat (higher L-value: 37·0 v. 35·6; P 〈 0·05) with a higher shear force value (64·0 v. 46·4 Newton; P 〈 0·01) compared with control animals. It seems likely that mature cattle receiving cimaterol respond similarly to younger growing animals.
    Type of Medium: Online Resource
    ISSN: 1357-7298 , 1748-748X
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 1995
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  • 10
    Online Resource
    Online Resource
    Cambridge University Press (CUP) ; 1999
    In:  Animal Science Vol. 69, No. 1 ( 1999-08), p. 275-285
    In: Animal Science, Cambridge University Press (CUP), Vol. 69, No. 1 ( 1999-08), p. 275-285
    Abstract: To optimize performance and carcass quality of Belgian Blue double-muscled bulls four phased feeding regimens were evaluated. All diets were offered ad libitum and consisted of 650 g concentrates and 350 g maize silage per kg dry matter (DM). Total period was divided into three phases (ca. 360 to 460, 460 to 570 and 570 to 680 kg). All four groups received the same maize silage but the different energy and protein densities were applied using different concentrates. During the three phases, the negative control (NC) group constantly received a diet with a low protein and a moderate energy density; the second group (DP) received with each phase diets with decreasing protein density while the energy density remained moderate; the third group (IE) received diets with increasing energy density at a constantly high protein density and the last group (DPIE) received diets which decreased in protein and increased in energy density. The NC group grew significantly slower during the first interval (1·37 v. 1·62 kg/day for the other groups), resulting in a significantly longer total finishing period. During the third period IE had the slowest growth. The NC group needed 21 and 20 days more than the DP and DPIE groups, respectively, to reach the same slaughter weight. The NC group had the lowest DM intake during the first interval, the IE group during the third interval. No significant differences (P 〉 0·05) were found for food conversion, when expressed as DM or NEF (net energy for fattening). IE had the worst protein conversion while NC had the best, with the two other groups being intermediate. The only important significant difference concerning carcass quality was the improved dressing proportion of the IE and DP groups. Although significant, differences in the fat content of the m. longissimus thoracis are of minor importance. These results proved that for Belgian Blue double-muscled bulls, protein density of the diet can be decreased with increasing live weight, while energy should be increased to give optimal performance.
    Type of Medium: Online Resource
    ISSN: 1357-7298 , 1748-748X
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 1999
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