In:
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, American Physiological Society, Vol. 277, No. 6 ( 1999-12-01), p. R1605-R1611
Abstract:
Endothelin (ET) from a nontetrapod species has never been characterized, either structurally or biologically. A single molecular form of trout ET with 21-amino-acid residues was isolated in pure form from an extract of the kidney of the steelhead trout, Oncorhynchus mykissand its primary structure established as Cys-Ser-Cys-Ala-Thr-Phe-Leu-Asp-Lys-Glu 10 -Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile 20 -Trp. This amino acid sequence shows only three substitutions (Ala 4 →Ser, Thr 5 →Ser, and Phe 6 →Trp) compared with human ET-2, demonstrating that the structure of the peptide has been well conserved during evolution and that the pathway of posttranslational processing of preproendothelin in the trout is probably similar to that in mammals. Synthetic trout ET produced concentration-dependent constrictions of isolated rings of vascular tissue from trout efferent branchial artery (EBA; pD 2 = 7.90 ± 0.06, n = 5), caeliacomesenteric artery (pD 2 = 8.03 ± 0.04, n = 4), anterior cardinal vein (ACV; pD 2 = 8.57 ± 0.25, n = 4), and rat abdominal aorta (AO; pD 2 = 8.86 ± 0.08, n = 7). Trout and rat vessels were more sensitive to mammalian ET-1 than to trout ET (pD 2 for human ET-1 in: EBA = 9.12 ± 0.14; ACV = 9.90 ± 0.15; AO = 8.86 ± 0.08), but there was no significant difference in the maximum tension produced by either peptide in these vessels.
Type of Medium:
Online Resource
ISSN:
0363-6119
,
1522-1490
DOI:
10.1152/ajpregu.1999.277.6.R1605
Language:
English
Publisher:
American Physiological Society
Publication Date:
1999
detail.hit.zdb_id:
603839-6
detail.hit.zdb_id:
1477297-8
SSG:
12
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