In:
Insect Molecular Biology, Wiley, Vol. 16, No. 5 ( 2007-10), p. 573-580
Abstract:
Germ‐line transformation of a major agricultural pest, the Mexican fruit fly ( Anastrepha ludens Loew, Mexfly), was achieved using composite piggyBac transposable elements marked with green, yellow and red fluorescent proteins (CopGreen, PhiYFP and J‐Red). We also investigated the possibility of generating transposon‐free insertions, in order to address potential concerns relating to proposed field use of transgenic Mexfly. We describe a highly efficient method for transforming Mexfly, compare efficiency of piggyBac terminal sequences for transformation and also describe the derivation of a transposon‐free insertion line. The development of an efficient transformation system for Mexfly holds great promise for improved applications of the sterile insect technique, a major component of the present control measures for this economically important pest species.
Type of Medium:
Online Resource
ISSN:
0962-1075
,
1365-2583
DOI:
10.1111/imb.2007.16.issue-5
DOI:
10.1111/j.1365-2583.2007.00752.x
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
2020348-2
SSG:
12
Permalink