In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 1 ( 2022-1-10), p. e1010001-
Abstract:
Invasive Pulmonary Aspergillosis, which is caused by the filamentous fungus Aspergillus fumigatus , is a life-threatening infection for immunosuppressed patients. Chromatin structure regulation is important for genome stability maintenance and has the potential to drive genome rearrangements and affect virulence and pathogenesis of pathogens. Here, we performed the first A . fumigatus global chromatin profiling of two histone modifications, H3K4me3 and H3K9me3, focusing on the two most investigated A . fumigatus clinical isolates, Af293 and CEA17. In eukaryotes, H3K4me3 is associated with active transcription, while H3K9me3 often marks silent genes, DNA repeats, and transposons. We found that H3K4me3 deposition is similar between the two isolates, while H3K9me3 is more variable and does not always represent transcriptional silencing. Our work uncovered striking differences in the number, locations, and expression of transposable elements between Af293 and CEA17, and the differences are correlated with H3K9me3 modifications and higher genomic variations among strains of Af293 background. Moreover, we further showed that the Af293 strains from different laboratories actually differ in their genome contents and found a frequently lost region in chromosome VIII. For one such Af293 variant, we identified the chromosomal changes and demonstrated their impacts on its secondary metabolites production, growth and virulence. Overall, our findings not only emphasize the influence of genome heterogeneity on A . fumigatus fitness, but also caution about unnoticed chromosomal variations among common laboratory strains.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010001
DOI:
10.1371/journal.pgen.1010001.g001
DOI:
10.1371/journal.pgen.1010001.g002
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10.1371/journal.pgen.1010001.g003
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10.1371/journal.pgen.1010001.g004
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10.1371/journal.pgen.1010001.g005
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10.1371/journal.pgen.1010001.g006
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10.1371/journal.pgen.1010001.g007
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10.1371/journal.pgen.1010001.s001
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10.1371/journal.pgen.1010001.s002
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10.1371/journal.pgen.1010001.s003
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10.1371/journal.pgen.1010001.s004
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10.1371/journal.pgen.1010001.s005
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10.1371/journal.pgen.1010001.s006
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10.1371/journal.pgen.1010001.s007
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10.1371/journal.pgen.1010001.s008
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10.1371/journal.pgen.1010001.s009
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10.1371/journal.pgen.1010001.s010
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10.1371/journal.pgen.1010001.s011
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10.1371/journal.pgen.1010001.s012
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10.1371/journal.pgen.1010001.s013
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10.1371/journal.pgen.1010001.s014
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10.1371/journal.pgen.1010001.s015
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10.1371/journal.pgen.1010001.s016
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10.1371/journal.pgen.1010001.s017
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10.1371/journal.pgen.1010001.s018
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10.1371/journal.pgen.1010001.s019
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10.1371/journal.pgen.1010001.s020
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10.1371/journal.pgen.1010001.s021
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10.1371/journal.pgen.1010001.s022
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10.1371/journal.pgen.1010001.s023
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10.1371/journal.pgen.1010001.s024
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10.1371/journal.pgen.1010001.s025
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10.1371/journal.pgen.1010001.s026
DOI:
10.1371/journal.pgen.1010001.s027
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10.1371/journal.pgen.1010001.s028
DOI:
10.1371/journal.pgen.1010001.r001
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10.1371/journal.pgen.1010001.r002
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10.1371/journal.pgen.1010001.r003
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10.1371/journal.pgen.1010001.r004
DOI:
10.1371/journal.pgen.1010001.r005
DOI:
10.1371/journal.pgen.1010001.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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