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  • 1
    In: Journal of Molecular Endocrinology, Bioscientifica, ( 2023-09)
    Kurzfassung: Suboptimal in utero environments impact fetal birthweight and the metabolic health trajectory of the adult offspring. We reported that when metabolically challenged by a high-fat diet, placental mTORKO (mTORKOpl) adult female offspring develop obesity and insulin resistance, whereas placental TSC2KO (TSC2KOpl) female offspring are protected from obesity. Here, we investigated whether reducing or in-creasing placental mTOR signaling in utero alters programming of adult offspring metabolic tissues pre-ceding a metabolic challenge. Adult male and female mTORKOpl, TSC2KOpl and respective controls on a normal chow diet were subjected to an acute intraperitoneal insulin injection. Upon insulin stimulation, insulin signaling via phosphorylation of Akt and nutrient sensing via phosphorylation of mTOR target ribosomal S6 were evaluated in the offspring liver, white adipose tissue, and skeletal muscle. We observed significant changes only in the liver signaling. In the male mTORKOpl adult offspring liver, insulin stimulated phospho-Akt was enhanced compared to controls. Phospho-S6 was basally increased in the mTORKOpl female offspring liver compared to controls and did not increase further in response to insulin. RNA se-quencing of offspring liver identified mTORC1 programming-mediated differentially expressed genes (DEGs). The expression of major urinary protein 1 (Mup1) was altered in female mTORKOpl and TSC2KOpl offspring livers and we show that Mup1 may be regulated by mTOR, overnutrition, and fasting status. In summary, deletion of placental mTOR nutrient sensing in utero programs hepatic response to insulin action in a sexually dimorphic manner. Additionally, we highlight a role for hepatic and circulating Mup1 in glucose homeostasis that warrants further investigation.
    Materialart: Online-Ressource
    ISSN: 0952-5041 , 1479-6813
    Sprache: Unbekannt
    Verlag: Bioscientifica
    Publikationsdatum: 2023
    ZDB Id: 1478171-2
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    In: Cell Reports, Elsevier BV, Vol. 40, No. 11 ( 2022-09), p. 111321-
    Materialart: Online-Ressource
    ISSN: 2211-1247
    Sprache: Englisch
    Verlag: Elsevier BV
    Publikationsdatum: 2022
    ZDB Id: 2649101-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    American Diabetes Association ; 2023
    In:  Diabetes Vol. 72, No. Supplement_1 ( 2023-06-20)
    In: Diabetes, American Diabetes Association, Vol. 72, No. Supplement_1 ( 2023-06-20)
    Kurzfassung: Fetal Growth Restriction (FGR) is a determinant for the development of chronic diseases, such as type II diabetes in adulthood. FGR is caused by the failure of the placenta to provide adequate nutrients and oxygen for fetal development. mTOR regulates cell metabolism and growth, and it is reduced in the FGR placenta. We previously reported that placental mTOR ablation induces insulin resistance and glucose intolerance in adult mice. We hypothesize that mTOR signaling regulates glucose utilization in the placenta, which stores glucose reservoirs as glycogen. Appropriate localization and breakdown of glycogen cells is crucial for normal fetal growth. We leveraged our mouse model lacking placental mTOR signaling to ask whether glycogen cell number was altered. First, we found that critical glycogenesis and glycogenolysis enzymes, as well as the glucagon receptor, were expressed in placental tissue, indicating the capability for glycogen cell synthesis and breakdown, like glycogen-rich tissues such as the liver. Next, we show increased glycogen cell counts in the junctional zone of the mTORKO placenta compared to littermate controls by IHC. However, whole placental glycogen content, measured by ELISA, demonstrates no changes between KO and control. These data suggest that mTOR signaling modulates the localization and migration of glycogen cells during high metabolic needs. Glucagon mRNA is expressed in the placenta, with significant increases in the male KO placenta, indicating the placenta may produce its own glucagon. However, IHC staining indicates increased glucagon levels in female KO placenta in the labyrinth zone. Together, these data suggest that the placenta may have the capability to regulate its own glycogen breakdown by altering glucagon expression. Our results indicate that the FGR placenta induced by mTOR deletion has altered glycogen cell content and mislocalization, which mimics the phenotypes of placenta impacted by gestational diabetes, suggesting a shared mechanistic pathway. Disclosure B. Clifton: None. M. Beetch: None. E. Alejandro: None. Funding National Institutes of Health (R01DK115720)
    Materialart: Online-Ressource
    ISSN: 0012-1797
    Sprache: Englisch
    Verlag: American Diabetes Association
    Publikationsdatum: 2023
    ZDB Id: 1501252-9
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    In: BMJ, BMJ
    Kurzfassung: To determine whether medical errors, family experience, and communication processes improved after implementation of an intervention to standardize the structure of healthcare provider-family communication on family centered rounds. Design Prospective, multicenter before and after intervention study. Setting Pediatric inpatient units in seven North American hospitals, 17 December 2014 to 3 January 2017. Participants All patients admitted to study units (3106 admissions, 13171 patient days); 2148 parents or caregivers, 435 nurses, 203 medical students, and 586 residents. Intervention Families, nurses, and physicians coproduced an intervention to standardize healthcare provider-family communication on ward rounds (“family centered rounds”), which included structured, high reliability communication on bedside rounds emphasizing health literacy, family engagement, and bidirectional communication; structured, written real-time summaries of rounds; a formal training programme for healthcare providers; and strategies to support teamwork, implementation, and process improvement. Main outcome measures Medical errors (primary outcome), including harmful errors (preventable adverse events) and non-harmful errors, modeled using Poisson regression and generalized estimating equations clustered by site; family experience; and communication processes (eg, family engagement on rounds). Errors were measured via an established systematic surveillance methodology including family safety reporting. Results The overall rate of medical errors (per 1000 patient days) was unchanged (41.2 (95% confidence interval 31.2 to 54.5) pre-intervention v 35.8 (26.9 to 47.7) post-intervention, P=0.21), but harmful errors (preventable adverse events) decreased by 37.9% (20.7 (15.3 to 28.1) v 12.9 (8.9 to 18.6), P=0.01) post-intervention. Non-preventable adverse events also decreased (12.6 (8.9 to 17.9) v 5.2 (3.1 to 8.8), P=0.003). Top box (eg, “excellent”) ratings for six of 25 components of family reported experience improved; none worsened. Family centered rounds occurred more frequently (72.2% (53.5% to 85.4%) v 82.8% (64.9% to 92.6%), P=0.02). Family engagement 55.6% (32.9% to 76.2%) v 66.7% (43.0% to 84.1%), P=0.04) and nurse engagement (20.4% (7.0% to 46.6%) v 35.5% (17.0% to 59.6%), P=0.03) on rounds improved. Families expressing concerns at the start of rounds (18.2% (5.6% to 45.3%) v 37.7% (17.6% to 63.3%), P=0.03) and reading back plans (4.7% (0.7% to 25.2%) v 26.5% (12.7% to 7.3%), P=0.02) increased. Trainee teaching and the duration of rounds did not change significantly. Conclusions Although overall errors were unchanged, harmful medical errors decreased and family experience and communication processes improved after implementation of a structured communication intervention for family centered rounds coproduced by families, nurses, and physicians. Family centered care processes may improve safety and quality of care without negatively impacting teaching or duration of rounds. Trial registration ClinicalTrials.gov NCT02320175 .
    Materialart: Online-Ressource
    ISSN: 0959-8138 , 1756-1833
    Sprache: Englisch
    Verlag: BMJ
    Publikationsdatum: 2018
    ZDB Id: 1479799-9
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    In: JAMA Pediatrics, American Medical Association (AMA), Vol. 171, No. 4 ( 2017-04-01), p. 372-
    Materialart: Online-Ressource
    ISSN: 2168-6203
    Sprache: Englisch
    Verlag: American Medical Association (AMA)
    Publikationsdatum: 2017
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    Wiley ; 2022
    In:  Annals of the New York Academy of Sciences Vol. 1513, No. 1 ( 2022-07), p. 89-107
    In: Annals of the New York Academy of Sciences, Wiley, Vol. 1513, No. 1 ( 2022-07), p. 89-107
    Kurzfassung: While mathematics anxiety (MA) has been widely researched in recent decades, this study addresses significant gaps: namely, research that explores the relationship between MA and self‐reported mathematics experiences; samples adults with a range of MA levels; and controls for general anxiety. Additionally, the study sampled deaf and hard of hearing (DHH) students, whose diverse life and educational experiences often differ from hearing students’. We investigated whether DHH students’ experiences with mathematics (i.e., parental behaviors, school environment, and mathematics feelings) and demographic variables (i.e., hearing status, age, and gender) predict their MA, and whether these relationships differ from those in hearing students. Self‐report questionnaires were completed by 296 DHH and hearing college students. Linear regression analyses controlling for general anxiety led to the following inference: DHH students who reported more positive attitudes toward mathematics and school environments demonstrated higher MA. Also, the relationships between mathematics feelings, parental behaviors, and MA differed between DHH and hearing students. Logistic regression analyses showed no contribution of MA to students’ likelihood of pursuing STEM degrees in either DHH or between DHH and hearing groups. Overall, this work breaks new ground in the study of MA in DHH students and challenges standard views of the relationships between MA and individual experiences.
    Materialart: Online-Ressource
    ISSN: 0077-8923 , 1749-6632
    URL: Issue
    RVK:
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2022
    ZDB Id: 2834079-6
    ZDB Id: 211003-9
    ZDB Id: 2071584-5
    SSG: 11
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    In: The Joint Commission Journal on Quality and Patient Safety, Elsevier BV, Vol. 43, No. 7 ( 2017-07), p. 319-329
    Materialart: Online-Ressource
    ISSN: 1553-7250
    Sprache: Englisch
    Verlag: Elsevier BV
    Publikationsdatum: 2017
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    In: Journal of Cardiovascular Electrophysiology, Wiley, Vol. 34, No. 5 ( 2023-05), p. 1196-1205
    Kurzfassung: Most patients undergoing a left atrial appendage occlusion (LAAO) procedure are admitted for overnight observation. A same‐day discharge strategy offers the opportunity to improve resource utilization without compromising patient safety. We compared the patient safety outcomes and post‐discharge complications between same‐day discharge versus hospital admission (HA) ( 〉 1 day) in patients undergoing LAAO procedure. Methods A systematic search of MEDLINE and Embase was conducted. Outcomes of interest included peri‐procedural complications, re‐admissions, discharge complications including major bleeding and vascular complications, ischemic stroke, all‐cause mortality, and peri‐device leak 〉 5 mm. Mantel–Haenszel risk ratios (RRs) with 95% CIs were calculated. Results A total of seven observational studies met the inclusion criteria. There was no statistically significant difference between same‐day discharge versus HA regarding readmission (RR: 0.61; 95% confidence interval [CI]: [0.29–1.31] ; p  = .21), ischemic stroke after discharge (RR: 1.16; 95% CI: [0.49–2.73]), peri‐device leak 〉 5 mm (RR: 1.27; 95% CI: [0.42–3.85], and all‐cause mortality (RR: 0.60; 95% CI: [0.36–1.02] ). The same‐day discharge study group had significantly lower major bleeding or vascular complications (RR: 0.71; 95% CI: [0.54–0.94]). Conclusions This meta‐analysis of seven observational studies showed no significant difference in patient safety outcomes and post‐discharge complications between same‐day discharge versus HA. These findings provide a solid basis to perform a randomized control trial to eliminate any potential confounders.
    Materialart: Online-Ressource
    ISSN: 1045-3873 , 1540-8167
    URL: Issue
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2023
    ZDB Id: 2037519-0
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Online-Ressource
    Online-Ressource
    BirdLife Australia, Ltd. ; 2017
    In:  Australian Field Ornithology ( 2017), p. 30-34
    In: Australian Field Ornithology, BirdLife Australia, Ltd., ( 2017), p. 30-34
    Materialart: Online-Ressource
    Sprache: Unbekannt
    Verlag: BirdLife Australia, Ltd.
    Publikationsdatum: 2017
    Standort Signatur Einschränkungen Verfügbarkeit
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