In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 9 ( 2021-9-30), p. e0257863-
Abstract:
The endophytic bacterium Burkholderia contaminans NZ was isolated from jute, which is an important fiber-producing plant. This bacterium exhibits significant growth promotion activity in in vivo pot experiments, and like other plant growth-promoting (PGP) bacteria fixes nitrogen, produces indole acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. B . contaminans NZ is considered to exert a promising growth inhibitory effect on Macrophomina phaseolina , a phytopathogen responsible for infecting hundreds of crops worldwide. This study aimed to identify the possibility of B . contaminans NZ as a safe biocontrol agent and assess its effectiveness in suppressing phytopathogenic fungi, especially M . phaseolina . Co-culture of M . phaseolina with B . contaminans NZ on both solid and liquid media revealed appreciable growth suppression of M . phaseolina and its chromogenic aberration in liquid culture. Genome mining of B . contaminans NZ using NaPDoS and antiSMASH revealed gene clusters that displayed 100% similarity for cytotoxic and antifungal substances, such as pyrrolnitrin. GC-MS analysis of B . contaminans NZ culture extracts revealed various bioactive compounds, including catechol; 9,10-dihydro-12’-hydroxy-2’-methyl-5’-(phenylmethyl)- ergotaman 3’,6’,18-trione; 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one; 1-(1,6-Dioxooctadecyl)- pyrrolidine; 9-Octadecenamide; and 2- methoxy- phenol. These compounds reportedly exhibit tyrosinase inhibitory, antifungal, and antibiotic activities. Using a more targeted approach, an RP-HPLC purified fraction was analyzed by LC-MS, confirming the existence of pyrrolnitrin in the B . contaminans NZ extract. Secondary metabolites, such as catechol and ergotaman, have been predicted to inhibit melanin synthesis in M . phaseolina . Thus, B . contaminans NZ appears to inhibit phytopathogens by apparently impairing melanin synthesis and other potential biochemical pathways, exhibiting considerable fungistatic activity.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0257863
DOI:
10.1371/journal.pone.0257863.g001
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10.1371/journal.pone.0257863.g002
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10.1371/journal.pone.0257863.g003
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10.1371/journal.pone.0257863.g004
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10.1371/journal.pone.0257863.g005
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10.1371/journal.pone.0257863.t001
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10.1371/journal.pone.0257863.t002
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10.1371/journal.pone.0257863.t003
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10.1371/journal.pone.0257863.t004
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10.1371/journal.pone.0257863.t005
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10.1371/journal.pone.0257863.s001
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10.1371/journal.pone.0257863.s002
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10.1371/journal.pone.0257863.s003
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10.1371/journal.pone.0257863.s004
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10.1371/journal.pone.0257863.s005
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10.1371/journal.pone.0257863.s006
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10.1371/journal.pone.0257863.s007
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10.1371/journal.pone.0257863.s008
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10.1371/journal.pone.0257863.s009
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10.1371/journal.pone.0257863.s010
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10.1371/journal.pone.0257863.r001
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10.1371/journal.pone.0257863.r002
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10.1371/journal.pone.0257863.r003
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10.1371/journal.pone.0257863.r004
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10.1371/journal.pone.0257863.r005
DOI:
10.1371/journal.pone.0257863.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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