In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_12 ( 2013-12-01), p. 4744-4749
Abstract:
A pink-pigmented, chemo-organotrophic bacterium, designated strain 03SUJ4 T , was isolated from the freshwater of Juam reservoir, Republic of Korea (35° 03′ 43′′ N 127° 14′ 15′′ E). Cells were aerobic, Gram-reaction-negative and non-motile rods. Strain 03SUJ4 T grew at pH 6–7 (optimum, pH 6) and at 15–30 °C (optimum, 25 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus Terriglobus , showing sequence similarities of 97.09 % and 96.82 % to Terriglobus roseus DSM 18391 T and Terriglobus saanensis SP1PR4 T , respectively. Low rpoB gene sequence similarity with members of the genus Terriglobus and different fingerprints with the repetitive primers BOX, ERIC and REP indicated that the isolate represented a novel species of the genus Terriglobus . The major cellular fatty acids were iso-C 15 : 0 , C 16 : 0 , C 20 : 1 ω9 c , C 14 : 0 and summed feature 3 (C 16 : 1 ω7 c /C 16 : 1 ω6 c ). The DNA G+C content of strain 03SUJ4 T was 63.2±0.1 mol% (mean± sd of three determinations). The predominant menaquinone was MK-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. Several phenotypic characteristics served to differentiate the novel isolate from recognized members of the genus Terriglobus . On the basis of the evidence presented in this study, a novel species, Terriglobus aquaticus sp. nov. is proposed for strain 03SUJ4 T ( = KCTC 23332 T = JCM 17517 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.050724-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
2056611-6
detail.hit.zdb_id:
2002336-4
SSG:
12
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