In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2021-2-19), p. e0247160-
Abstract:
Treatment with hydrogen peroxide (H 2 O 2 ) raises the hatching rate through the development and diapause termination of Artemia cysts. To comprehend the upstream genetic regulation of diapause termination activated by exterior H 2 O 2 elements, an Illumina RNA-seq analysis was performed to recognize and assess comparative transcript amounts to explore the genetic regulation of H 2 O 2 in starting the diapause termination of cysts in Artemia salina . We examined three groupings treated with no H 2 O 2 (control), 180 μM H 2 O 2 (low) and 1800 μM H 2 O 2 (high). The results showed a total of 114,057 unigenes were identified, 41.22% of which were functionally annotated in at least one particular database. When compared to control group, 34 and 98 differentially expressed genes (DEGs) were upregulated in 180 μM and 1800 μM H 2 O 2 treatments, respectively. On the other hand, 162 and 30 DEGs were downregulated in the 180 μM and 1800 μM H 2 O 2 treatments, respectively. Cluster analysis of DEGs demonstrated significant patterns among these types of 3 groups. GO and KEGG enrichment analysis showed the DEGs involved in the regulation of blood coagulation (GO: 0030193; GO: 0050818), regulation of wound healing (GO:0061041), regulation of hemostasis (GO: 1900046), antigen processing and presentation (KO04612), the Hippo signaling pathway (KO04391), as well as the MAPK signaling pathway (KO04010). This research helped to define the diapause-related transcriptomes of Artemia cysts using RNA-seq technology, which might fill up a gap in the prevailing body of knowledge.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0247160
DOI:
10.1371/journal.pone.0247160.g001
DOI:
10.1371/journal.pone.0247160.g002
DOI:
10.1371/journal.pone.0247160.g003
DOI:
10.1371/journal.pone.0247160.g004
DOI:
10.1371/journal.pone.0247160.t001
DOI:
10.1371/journal.pone.0247160.t002
DOI:
10.1371/journal.pone.0247160.t003
DOI:
10.1371/journal.pone.0247160.t004
DOI:
10.1371/journal.pone.0247160.s001
DOI:
10.1371/journal.pone.0247160.s002
DOI:
10.1371/journal.pone.0247160.s003
DOI:
10.1371/journal.pone.0247160.s004
DOI:
10.1371/journal.pone.0247160.s005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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