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  • 1
    Online Resource
    Online Resource
    Erratum to: The Interplay Between NSAIDs and Candida albicans on the Gastrointestinal Tract of Guinea Pigs
    Springer Science and Business Media LLC ; 2013
    In:  Mycopathologia Vol. 176, No. 1-2 ( 2013-8), p. 171-173
    Details
    In: Mycopathologia, Springer Science and Business Media LLC, Vol. 176, No. 1-2 ( 2013-8), p. 171-173
    Type of Medium: Online Resource
    ISSN: 0301-486X , 1573-0832
    URL: Article
    DOI: 10.1007/s11046-013-9659-7
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2013
    detail.hit.zdb_id: 2003647-4
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    In Vitro Antibacterial Susceptibility of Different Pathogens to Thirty Nano-Polyoxometalates
    MDPI AG ; 2021
    In:  Pharmaceuticals Vol. 15, No. 1 ( 2021-12-27), p. 33-
    Details
    In: Pharmaceuticals, MDPI AG, Vol. 15, No. 1 ( 2021-12-27), p. 33-
    Abstract: Due to their unique properties, nano-polyoxometalates (POMs) can be alternative chemotherapeutic agents instrumental in designing new antibiotics. In this research, we synthesized and characterized “smart” nanocompounds and validated their antibacterial effects in order to formulate and implement potential new drugs. We characterized thirty POMs in terms of antibacterial activity–structure relationship. The antibacterial effects of these compounds are directly dependent upon their structure and the type of bacterial strain tested. We identified three POMs that presented sound antibacterial activity against S. aureus, B. cereus, E. coli, S. enteritidis and P. aeruginosa strains. A newly synthesized compound K6[(VO)SiMo2W9O39]·11H2O (POM 7) presented antibacterial activity only against S. aureus (ATCC 6538P). Twelve POMs exerted antibacterial effects against both Gram-positive and Gram-negative strains. Only one POM (a cluster derivatized with organometallic fragments) exhibited a stronger effect compared to amoxicillin. New studies in terms of selectivity and specificity are required to clarify these extremely important aspects needed to be considered in drug design.
    Type of Medium: Online Resource
    ISSN: 1424-8247
    URL: Article
    DOI: 10.3390/ph15010033
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2193542-7
    SSG: 15,3
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  • 3
    Online Resource
    Online Resource
    In vitro susceptibility of prototheca unicellular algae to resorcin and rivanol
    Digital ProScholar Media ; 2020
    In:  Romanian Biotechnological Letters Vol. 25, No. 2 ( 2020-04-30), p. 1440-1447
    Details
    In: Romanian Biotechnological Letters, Digital ProScholar Media, Vol. 25, No. 2 ( 2020-04-30), p. 1440-1447
    Type of Medium: Online Resource
    ISSN: 1224-5984 , 2248-3942
    URL: Journal
    URL: Article
    DOI: 10.25083/rbl
    DOI: 10.25083/rbl/25.2/1440.1447
    Language: Unknown
    Publisher: Digital ProScholar Media
    Publication Date: 2020
    detail.hit.zdb_id: 2507808-2
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  • 4
    Online Resource
    Online Resource
    Efficacy of natural formulations in bovine mastitis pathology: alternative solution to antibiotic treatment
    Walter de Gruyter GmbH ; 2020
    In:  Journal of Veterinary Research Vol. 64, No. 4 ( 2020-10-20), p. 523-529
    Details
    In: Journal of Veterinary Research, Walter de Gruyter GmbH, Vol. 64, No. 4 ( 2020-10-20), p. 523-529
    Abstract: Bovine mastitis is an inflammatory disease of the udder that causes important economic losses in the animal breeding and dairy product industries. Nowadays, the conventional livestock antibiotic treatments are slowly being replaced by alternative treatments. In this context, the main aim of this study was to evaluate the efficacy of natural products in alternative treatment of bovine mastitis. Material and Methods Two natural formulations with previously suggested in vitro antimicrobial effect were tested in vivo on mastitic cows. Animals with a positive diagnosis for mastitis (n = 20) were divided into three treatment groups: two groups (n = 8) were administered formulations of propolis, alcoholic extracts of Brewers Gold and Perle hops, plum lichen, common mallow, marigold, absinthe wormwood, black poplar buds, lemon balm, and essential oils of oregano, lavender, and rosemary designated R4 and R7 (differing only in the latter being more concentrated) and one group (n = 4) a conventional antibiotic mixture. In vivo efficacy of treatments was evaluated by somatic cell and standard plate counts, the treatment being considered efficacious when both parameters were under the maximum limit. Results R7 was effective in the most cases, being therapeutically bactericidal in six out of eight cows, while R4 gave good results in three out of eight cows, and conventional antibiotics cured one out of four. Conclusion These results suggest the possible therapeutic potential of these natural products in bovine mastitis.
    Type of Medium: Online Resource
    ISSN: 2450-8608
    URL: Article
    DOI: 10.2478/jvetres-2020-0067
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2855010-9
    SSG: 22
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  • 5
    Online Resource
    Online Resource
    Antibacterial Activity of Pollen Extracts on Pathogenic Microflora from Milk
    University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca ; 2016
    In:  Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies Vol. 73, No. 2 ( 2016-11-28), p. 261-
    Details
    In: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Vol. 73, No. 2 ( 2016-11-28), p. 261-
    Abstract: Bee-pollen was used for thousands of years as functional food and medicinal plant product. Various beneficial effects were attributed to it and its consumption was increased over the years. The tests have been made on 16 pollen samples of the following families: Rosaceae, Salicaceae, Fabaceae, Tiliaceae, Asteraceae, Brassicaceae and two polifloral pollen assortment, using difuzimetric method. Among the tested bacteria, the Onobrychis viciifolia pollen has the highest sensitivity 11.86±1.79 mm while the low antibacterial activity was registered for Brassica sp. pollen  8.65±2.65 mm. As the use of antibiotic substances over a long period of time resulted in larger doses of residues in milk and representing a potential biohazard, the use of ethanol extracts from beepollen is a real alternative in the treatment of cows with various diseases.
    Type of Medium: Online Resource
    ISSN: 1843-536X , 1843-5262
    URL: Article
    DOI: 10.15835/buasvmcn-asb:12296
    Language: Unknown
    Publisher: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
    Publication Date: 2016
    detail.hit.zdb_id: 2720548-4
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  • 6
    Online Resource
    Online Resource
    Prevalence and Antimicrobial Susceptibility Profiles of Pathogen Isolated from Bovine Mastitis Milk in Transylvania, Romania
    University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca ; 2016
    In:  Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine Vol. 73, No. 2 ( 2016-11-30), p. 329-
    Details
    In: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Vol. 73, No. 2 ( 2016-11-30), p. 329-
    Abstract:  Mastitis in cows, one of the most common and economically important infectious diseases of dairy cattle, all over the world, with significant impact due to economic losses, occurs when the udder becomes inflamed because the leukocytes are released into the mammary gland usually in response to bacteria invasion of the teat canal.The main objective of this study was to evaluate the in vitro antimicrobial susceptibility of bacteria isolated from milk in order to design specific control programs for bovine mastitis in this area.A total of 204 milk samples aseptically collected both from farms and private owners were processed during May 2014 and March 2016 within the Microbiology Laboratory of the Faculty of Veterinary Cluj-Napoca, Romania. The microbiological examination was carried out by inoculation on blood agar and MacConkey medium. After the overnight incubation in aerobic conditions, the identification of the isolates was performed using microscopic, cultural and biochemical methods. Biochemical identification was based on API 20 Biomerieux system. Susceptibility to antibiotics was evaluated using Kirby Bauer disk diffusion method on Mueller-Hinton agar; the antibiotics were represented by Amoxicillin and Clavulanic Acid, Ceftiofur, Florfenicol, Mastidiscs, Enrofloxacin, Penicillin and Tetracycline.Staphylococcus spp. was the most common isolated pathogen, in 54.9% of the specimens, followed by Streptococcus spp. in 20.1%, Escherichia coli in 10.78%, Klebsiella spp. in 8.34%, Bacillus spp. in 5.88%. The most frequent associations were represented by staphylococci-streptococci in 62.7% of the samples, followed by streptococci-bacillus in 19.8% of the samples. The most important etiological agents identified were Staphylococcus aureus, S uberis, Streptococcus agalactiae, and Escherichia coli. Antimicrobial susceptibility test for the total isolates revealed good sensitivity to Enrofloxacin, Mastidiscs and Amoxicillin and Clavulanic Acid. Resistance was observed for Penicillin and Tetracycline.The major mastitis pathogens identified was Staphylococcus aureus, while recurrent mastitis treatment was based on systemic and local administration of Enrofloxacin and Mastidiscs respectively.
    Type of Medium: Online Resource
    ISSN: 1843-5378 , 1843-5270
    URL: Article
    DOI: 10.15835/buasvmcn-vm:12199
    Language: Unknown
    Publisher: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
    Publication Date: 2016
    detail.hit.zdb_id: 2415710-7
    SSG: 22
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  • 7
    Online Resource
    Online Resource
    In Vitro Sensitivity Research Concerning Some Microorganisms at Hydroxyquinoline and Cupric Derivatives Deposited onto Hydroxyapatite
    University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca ; 2016
    In:  Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine Vol. 73, No. 2 ( 2016-11-30), p. 339-
    Details
    In: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Vol. 73, No. 2 ( 2016-11-30), p. 339-
    Abstract: ABSTRACT Introduction: The preparations based on hydroxyquinoline, in various combinations, are used in medicine, being shown to have an inhibitory effect against bacteria, molds, fungi, parasites, and viruses, but also having other beneficial effects mentioned in other medical conditions (anti-cancer, anti-degenerative, anti-inflammatory). Aims: In vitro susceptibility testing of microorganisms: bacteria (Gram positive and Gram negative), yeast (Candida spp.,) and unicellular algae (Prototheca spp.) at the preparations based on hydroxyquinoline (HQ) and its cupric derivatives deposited on hydroxyapatite (HAP). Materials and methods: There were tested microbial strains of the following genera: Escherichia, Staphylococcus, Micrococcus, Bacillus, Candida, and Prototheca. The tested products (developed in the Laboratory for Nanobiomaterials Synthesis, Center of Physical Chemistry, Faculty of Chemistry and Chemical Engineering, UBB Cluj-Napoca) were developed in three versions: 1) HQ–Cu2+–HAP1; 2) HQ–Cu2+–HAP2; and 3) NHQ–Cu2+–HAP2, where NHQ stands for nitro hydroxyquinoline. Determination of the inhibitory effect was conducted by diffusion technique in nutrient agar, according to CLSI 2013 standards, with necessary adaptations for testing of products made in the form of suspensions. Results: Following interpretation, it was found that the inhibition zones, arising from the antimicrobial effect of the tested products showed variability in size, depending on the test product and the microbial strain: Escherichia coli (8-10 mm), Staphylococcus sp. (17.6 - 23.2 mm), Micrococcus spp. (24.4 - 27.6 mm), Bacillus spp. (14.0 - 16.0 mm), Candida spp. (20.4 - 25.2 mm), Prototheca spp. (20.8 - 30.0 mm). From the three tested products, the best efficacy was found at the product no. 3 (NHQ – Cu2+ – HAP2), followed by no. 1 (HQ– Cu2+–HAP1) and no. 2 (HQ–Cu2+–HAP2). Conclusions: The inhibitory effect was bactericidal, manifested more intensively against Gram-positive bacteria, yeasts, and prototheca. Such products, prepared in the form of suspensions, may have practical application in the prevention and treatment of skin or hooves disorders. No resistance phenomena are recorded. Keywords: copper, hydroxyapatite, hydroxyquinoline, microorganisms, sensitivity.
    Type of Medium: Online Resource
    ISSN: 1843-5378 , 1843-5270
    URL: Article
    DOI: 10.15835/buasvmcn-vm:12203
    Language: Unknown
    Publisher: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
    Publication Date: 2016
    detail.hit.zdb_id: 2415710-7
    SSG: 22
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  • 8
    Online Resource
    Online Resource
    Preliminary Data on the In Vitro Effect of Copper-Based Compounds on Aerobic Bacterial Microflora Isolated from Sheep Footrot
    University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca ; 2018
    In:  Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine Vol. 75, No. 1 ( 2018-05-19), p. 83-
    Details
    In: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Vol. 75, No. 1 ( 2018-05-19), p. 83-
    Abstract: Contagious sheep footrot is an infectious disease with chronic evolution, usually enzootic, characterized by necrotic or necrotic-purulent inflammation of the soft tissues of the hoof, which causes partial or total detachment of the horn. Cu-based products represented by (I) 1-hydroxyquinoline + Cu on 5% hydroxyapatite, (II) 2 Hydroxyquinoline + Cu + NO3 on 5% Hydroxyapatite and (III) 3 Nitrohydroxyquinoline + Cu on 5% Hydroxyapatite were conditioned in aqueous suspension in a volume of 100 ml, pH 6,0 – 6,4. The products were placed into wells, 20 μL/well, along with 0.5 % copper sulphate solution (Cu control) and Enroxil and Oxitetracycline (control for antibiotics).The results demonstrated that 7 of the samples (58.33 %) are sensitive to the product I, 6 samples (50%) to the product III, 4 samples (33.33 %) to the product 2 and the 0.5% Copper sulphate solution only has a bacteriostatic effect. In comparison to the two antibiotics, 9 samples (75 %) are sensitive to enrofloxacin and only 5 samples (41.66 %) to oxytetracycline. Regarding the associations of microorganisms in the samples with resistance, we have found that these are represented by the most common germs of genus Bacillus, Corynebacterium and Gram negative bacteria, and rarer associations with Micrococcus and Staphylococcus.
    Type of Medium: Online Resource
    ISSN: 1843-5378 , 1843-5270
    URL: Article
    DOI: 10.15835/buasvmcn-vm:005317
    Language: Unknown
    Publisher: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
    Publication Date: 2018
    detail.hit.zdb_id: 2415710-7
    SSG: 22
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  • 9
    Online Resource
    Online Resource
    Gastrointestinal Trichocephalosis Associated With Candidiasis in a Pig
    University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca ; 2014
    In:  Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine Vol. 71, No. 2 ( 2014-11-28)
    Details
    In: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Vol. 71, No. 2 ( 2014-11-28)
    Type of Medium: Online Resource
    ISSN: 1843-5378 , 1843-5270
    URL: Article
    DOI: 10.15835/buasvmcn-vm:10698
    Language: Unknown
    Publisher: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
    Publication Date: 2014
    detail.hit.zdb_id: 2415710-7
    SSG: 22
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  • 10
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    In Vitro Preparation And Testing Of Anti-Salmonella Vaccine Against Abortion In Sheep
    University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca ; 2017
    In:  Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine Vol. 74, No. 1 ( 2017-05-18), p. 26-
    Details
    In: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Vol. 74, No. 1 ( 2017-05-18), p. 26-
    Abstract: Abstract: In March 2016, the microbiology laboratory of the Faculty of Veterinary Medicine in Cluj-Napoca, 3 type of antisalmonella vaccine for sheep were prepared. For type 1, 24 hours salmonella on brain heart infusion (BHI) broth culture, heat inactivated for 1 hour at 60°C, then with formaldehyde in a concentration of 3 ‰. Variant 2 - the culture supernatant obtained on solid BHI medium, washed with PBS, frozen-thawed 6 times, centrifuged at 4000 rpm for 15 minutes, filtered through 0.2 μm Millipore filter Orange Scientific. Variant 3 - suspension of cell walls remaining after centrifugation suspended in PBS and inactivated with formaldehyde.After the bacterial and fungal sterility control, the three types of vaccine were administered in rabbits by the subcutaneous route at a dose of 1 ml/individual along with 1 ml of plant extract adjuvant (decoction of Ewernia prunastri) sterilized by filtration.There have been two booster inoculations of the initial administration, 7 and 14 days after. Before each dose of vaccine, blood was sampled from the marginal auricular vein in order to control the immunogenicity by anti-somatic ˮOˮ serum antibody (Ab) titration using slow microplate agglutination test (Widal reaction). After three inoculations with the vaccine variant 1, Ab serum titer reached 1/128, and in types 2 and 3, 1/512 after 2 inoculations, decreasing to 1/256 after the second booster administered with no immunomodulator.
    Type of Medium: Online Resource
    ISSN: 1843-5378 , 1843-5270
    URL: Article
    DOI: 10.15835/buasvmcn-vm:12452
    Language: Unknown
    Publisher: University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
    Publication Date: 2017
    detail.hit.zdb_id: 2415710-7
    SSG: 22
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