In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 79, No. 5 ( 2013-03), p. 1428-1435
Abstract:
Determination of the complete nucleotide sequence of a cryptic plasmid, pMBLT00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC13302 revealed that it contains 20,721 bp, a G+C content of 38.7%, and 18 open reading frames. Comparative sequence and mung been nuclease analyses of pMBLT00 showed that pMBLT00 replicates via the theta replication mechanism. A new, stable Escherichia coli-Leuconostoc shuttle vector, pMBLT02, which was constructed from a theta-replicating pMBLT00 replicon and an erythromycin resistance gene of pE194, was successfully introduced into Leuconostoc , Lactococcus lactis , and Pediococcus . This shuttle vector was used to engineer Leuconostoc citreum 95 to overproduce d -lactate. The L. citreum 95 strain engineered using plasmid pMBLT02, which overexpresses d -lactate dehydrogenase, exhibited enhanced production of optically pure d -lactate (61 g/liter, which is 6 times greater than the amount produced by the control strain) when cultured in a reactor supplemented with 140 g/liter glucose. Therefore, the shuttle vector pMBLT02 can serve as a useful and stable plasmid vector for further development of a d -lactate overproduction system in other Leuconostoc strains and Lactococcus lactis .
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.03291-12
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2013
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
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