In:
NPG Asia Materials, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2019-12)
Abstract:
Due to their inherent hydrophobic and bioinert nature, synthetic degradable polymer-based membranes show inferior stem cell attachment, proliferation, and even differentiation. To overcome these limitations, bioinspired and osteopromotive polydopamine nanoparticle-incorporated fibrous membranes are developed via a two-step route: pH-induced polymerization of dopamine and co-electrospinning of polycaprolactone (PCL) with polydopamine nanoparticles (PDA NPs). Hybrid membranes with optimized PDA NP content exhibit high quantities of apatite deposition and prominent cytocompatibility (cell attachment, spreading and reproduction) and osteo-differentiation potential (alkaline phosphatase activity, calcium mineralization, and osteogenesis-related genes and protein expression) of human mesenchymal stem cells cultured without any growth factors. Importantly, in vivo assessments using a mouse calvarial critical-sized defect demonstrate that the engineered fibrous membranes remarkably boost bone reconstruction and regeneration. Accordingly, our bioinspired PCL-based hybrid fibrous membranes with robust osteoinductive ability can potentially be utilized as a clinically applicable candidate in guided tissue regeneration applications.
Type of Medium:
Online Resource
ISSN:
1884-4049
,
1884-4057
DOI:
10.1038/s41427-019-0139-5
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2019
detail.hit.zdb_id:
2608333-4
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