In:
Journal of Endocrinology, Bioscientifica, Vol. 185, No. 3 ( 2005-06), p. 497-505
Abstract:
Earlier studies have shown that germ cells or germ cell-conditioned media are capable of regulating α 2 -macroglobulin (α 2 -MG, a non-specific protease inhibitor) expression by Sertoli cells and hepatocytes cultured in vitro. These results illustrate a possible physiological link between testes and liver regarding α 2 -MG production. Using a series of surgical procedures including castration, hemicastration, and hepatectomy coupled with Northern blot and immunoblot analyses, we report herein that the surge in α 2 -MG expression in the liver in response to inflammation is indeed regulated, at least in part, by the testis via testosterone. It was found that hepatectomy induced at least a tenfold increase in the steady-state mRNA and protein production of α 2 -MG in the liver. However, castration induced a mild but not statistically significant induction of α 2 -MG in the liver in contrast to sham operation or hemicastration alone, when hemicastration alone could induce liver α 2 -MG production by almost fourfold. Perhaps most important of all, hepatectomy accompanied by castration significantly reduced the liver α 2 -MG response to the surgery-induced inflammation compared with hepatectomy alone, illustrating that the removal of the testicles can induce a loss of signal communications between the testis and the liver, rendering a significant loss of the α 2 -MG response to experimentally induced inflammation in the liver. Interestingly, this lack of response of the liver to surgery-induced inflammation regarding α 2 -MG production following castration could be restored, at least in part, by using testosterone implants placed subdermally 6 days prior to orchiectomy. Collectively, these results illustrate that a physiological link does indeed exist between the testis and the liver, and that testes per se can influence the liver in vivo α 2 -MG expression in response to inflammation possibly via testosterone or testosterone-induced biological factor(s).
Type of Medium:
Online Resource
ISSN:
0022-0795
,
1479-6805
Language:
Unknown
Publisher:
Bioscientifica
Publication Date:
2005
detail.hit.zdb_id:
1474892-7
Permalink