In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 61, No. 9 ( 2011-09-01), p. 2029-2034
Abstract:
A novel non-sporulating, non-motile, catalase- and oxidase-positive, strictly aerobic, Gram-positive-staining coccus, strain JSM 077023 T , was isolated from an intertidal sediment sample collected from Naozhou Island in the South China Sea, China. Growth occurred in the presence of 0.5–25 % (w/v) NaCl [optimum, 2–5 % (w/v) NaCl] and at pH 5.5–10.5 (optimum, pH 7.0–8.0) and at 4–45 °C (optimum, 30–35 °C). The major amino acid constituents of the cell wall were alanine, glycine and lysine. The major cellular fatty acids were anteiso-C 15 : 0 and iso-C 15 : 0 . The strain contained MK-7 and MK-6 as the predominant respiratory quinones and diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid as the polar lipids. The genomic DNA G+C content of strain JSM 077023 T was 41.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 077023 T should be assigned to the genus Jeotgalicoccus and was most closely related to the type strains of Jeotgalicoccus halotolerans (sequence similarity 99.0 %) and Jeotgalicoccus aerolatus (99.0 %), followed by Jeotgalicoccus coquinae (98.6 %) and Jeotgalicoccus psychrophilus (97.4 %). 16S rRNA gene sequence similarities of less than 97 % were observed with other species of the genus Jeotgalicoccus . Levels of DNA–DNA relatedness between strain JSM 077023 T and the type strains of J. halotolerans , J. aerolatus , J. coquinae and J. psychrophilus ranged from 36.8 to 22.7 %. The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain JSM 077023 T represents a novel species of the genus Jeotgalicoccus , for which the name Jeotgalicoccus nanhaiensis sp. nov. is proposed. The type strain is JSM 077023 T ( = DSM 23006 T = KCTC 13714 T ). An emended description of the genus Jeotgalicoccus is also presented.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.022871-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2011
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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