In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_12 ( 2015-12-01), p. 4716-4723
Abstract:
Seven strains, ICMP 19430 T , ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume Dipogon lignosus (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10–37 °C (optimum, 25–30 °C), at pH 4.0–9.0 (optimum, pH 6.0–7.0) and with 0–2 % (w/v) NaCl (optimum growth in the absence of NaCl). On the basis of 16S rRNA gene sequence analysis, the strains showed 99.0–99.5 % sequence similarity to the closest type strain, Burkholderia phytofirmans PsJN T , and 98.4–99.7 % sequence similarity to Burkholderia caledonica LMG 19076 T . The predominant fatty acids were C 18 : 1 ω7 c (21.0 % of the total fatty acids in strain ICMP 19430 T ), C 16 : 0 (19.1 %), C 17 : 0 cyclo (18.9 %), summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ; 10.7 %) and C 19 : 0 cyclo ω8 c (7.5 %). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was Q-8 and the DNA G+C content of strain ICMP 19430 T was 63.2 mol%. The DNA–DNA relatedness of the novel strains with respect to the closest neighbouring members of the genus Burkholderia was 55 % or less. On the basis of 16S rRNA and recA gene sequence similarities and chemotaxonomic and phenotypic data, these strains represent a novel symbiotic species in the genus Burkholderia , for which the name Burkholderia dipogonis sp. nov. is proposed, with the type strain ICMP 19430 T ( = LMG 28415 T = HAMBI 3637 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.000639
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
2056611-6
detail.hit.zdb_id:
2002336-4
SSG:
12
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