In:
Journal of Systematics and Evolution, Wiley, Vol. 49, No. 3 ( 2011-05), p. 261-266
Abstract:
Abstract In 2009, the Consortium for the Barcode of Life (CBOL) recommended the combination of rbcL and matK as the plant barcode based on assessments of recoverability, sequencing quality, and levels of species discrimination. Subsequently, based on a study of more than 6600 samples belonging to 193 families from seven phyla, the internal transcribed spacer (ITS) 2 locus was proposed as a universal barcode sequence for all major plant taxa used in traditional herbal medicine. Neither of these two studies was based on a detailed analysis of a particular family. Here, Zingiberaceae plants, including many closely related species, were used to compare the genetic divergence and species identification efficiency of ITS2, rbcL , matK , psbK – psbI , trnH – psbA , and rpoB . The results indicate that ITS2 has the highest interspecific divergence and significant differences between inter‐ and intraspecific divergence, whereas matK and rbcL have much lower divergence values. Among 260 species belonging to 30 genera in Zingiberaceae, the discrimination ability of the ITS2 locus was 99.5% at the genus level and 73.1% at the species level. Thus, we propose that ITS2 is the preferred DNA barcode sequence for identifying Zingiberaceae plants.
Type of Medium:
Online Resource
ISSN:
1674-4918
,
1759-6831
DOI:
10.1111/jse.2011.49.issue-3
DOI:
10.1111/j.1759-6831.2011.00133.x
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
2516638-4
SSG:
6,25
SSG:
12
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