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  • 1
    In: Surgical and Radiologic Anatomy, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 1989-3), p. 3-10
    Type of Medium: Online Resource
    ISSN: 0930-312X , 1279-8517
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 1989
    detail.hit.zdb_id: 1461974-X
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  • 2
    In: Diversity, MDPI AG, Vol. 14, No. 7 ( 2022-07-09), p. 552-
    Abstract: As the largest alpine peat swamp wetland distribution area in the world, the Zoige has important ecological functions, including water conservation and biodiversity maintenance. In the past 20 years, the regional ecological protection and restoration measures continuously strengthened under the leadership of the local government have led to gradual improvements in the ecological environment of the region. In this study, multisource satellite remote-sensing image data were used to carry out quantitative monitoring and assessment of the main ecological elements (vegetation and water), as well as the regional leading ecosystem service function in the Zoige. Combined with local ecological protection management policies and measures, we analyzed the characteristics and effectiveness of ecological protection. We compared the ecosystem change trends of the Zoige reserve and the county, from 2001 to 2020, and found that the fractional vegetation cover (FVC) of Zoige county has increased at a rate of 0.25%/year. The growth rate was highest between 2015 and 2020, and the growth rate of FVC in the Zoige Wetland National Nature Reserve is approximately 1.89-fold that of the whole county. The water area also shows similar variation characteristics. On the whole, the water conservation capacity of the Zoige showed a significant increase from 2001 to 2020. We used high-resolution satellite remote-sensing images to capture the details of land use changes brought about by local ecological protection policies and measures, and together with macroecological indicators, we reflected on the effectiveness of regional ecological protection measures. We observed that the ecological effects of nature reserves are more direct and rapid, and the amount of water conservation within the nature reserve is about 1 × 104 m3/km2 higher than that of the surrounding grasslands. Satellite remote-sensing images can not only capture the multiscale change information of ecological indicators, such as vegetation and water, in a timely manner, but can also help us to identify the effectiveness of conservation measures by distinguishing and analyzing the causes of these changes.
    Type of Medium: Online Resource
    ISSN: 1424-2818
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518137-3
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  • 3
    Online Resource
    Online Resource
    Frontiers Media SA ; 2021
    In:  Frontiers in Microbiology Vol. 12 ( 2021-11-29)
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 12 ( 2021-11-29)
    Abstract: The CRISPR diagnostic (CRISPR-Dx) technology that employs the trans -cleavage activities has shown great potential in diagnostic sensitivity, specificity, convenience, and portability, and has been recognized as the next-generation diagnostic methods. However, due to the lack of standardized definition of Cas trans -cleavage enzymatic units, it is difficult to standardize the present CRISPR-Dx systems, which have undoubtedly impeded the development of the CRISPR-Dx industry. To solve the problem, we here first systematically optimized the reaction systems for Cas12a, and then defined its trans -cleavage units ( trans U), which we believe will be of great importance and interest to researchers in both molecular diagnostic industry and basic research. Moreover, a simple protocol was provided to facilitate a step-by-step measurement of the Cas12a trans U, which can also act as a reference for the definition of the trans U for other Cas proteins.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2587354-4
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  • 4
    Online Resource
    Online Resource
    Elsevier BV ; 2014
    In:  Analytical Biochemistry Vol. 456 ( 2014-07), p. 53-60
    In: Analytical Biochemistry, Elsevier BV, Vol. 456 ( 2014-07), p. 53-60
    Type of Medium: Online Resource
    ISSN: 0003-2697
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2014
    detail.hit.zdb_id: 1461105-3
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  • 5
    In: Agronomy, MDPI AG, Vol. 13, No. 2 ( 2023-02-09), p. 501-
    Abstract: [Objective] This study aims to analyze the effects of the plant growth regulator Miantaijin (N,N-dimethyl piperidinium chloride and 2-N,N-diethylaminoethyl caproate) and planting density on yield and nitrogen (N), phosphorus (P), and potassium (K) uptake and accumulation in cotton. The results will clarify the high-yield cultivation techniques in the cotton direct seeding after wheat harvesting cropping system in the Yangtze River Basin. [Method] In 2017 and 2018, the cotton cultivar Guoxinzao 11-1 was planted at 3 densities (75,000, 90,000, and 105,000 plants·ha−1), and 3 Miantaijin doses (0, 1170, and 2340 mL·ha−1) were imposed. [Results] The results show that the highest yield (3551.3–3687.5 kg·ha−1) was achieved with a 90,000 and 105,000 plant·ha−1 density and 1170 mL·ha−1 of Miantaijin (seedling stage: 90 mL·ha−1, peak squaring stage: 180 mL·ha−1, peak flowering stage: 360 mL·ha−1, and peak bolling stage: 540 mL·ha−1). Under these conditions, the uptakes of N, P, and K were also the highest, up to 117.8 kg·ha−1, 77.4 kg·ha−1, and 116.4 kg·ha−1, respectively. N uptake was the highest from the peak flowering to peak squaring stage, while the highest uptakes of P and K were both detected from the peak squaring to peak flowering stages. We also found significant linear positive correlations between yield and the total absorptions and accumulations of N, P, and K, especially during the peak flowering–peak bolling stage. [Conclusions] The optimum dose of Miantaijin with a medium and high density could enhance the absorption of N, P, and K during the whole growth period of the cotton population, especially in the peak flowering–boll opening stage. This resulted in the highest yield of direct-seeded cotton after wheat harvesting.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 6
    In: Agriculture, MDPI AG, Vol. 12, No. 12 ( 2022-11-24), p. 1999-
    Abstract: As one of the most important edible legumes worldwide, faba bean can be grown for grain, feed, vegetable, fertilizer, medicine and deep processing. In this study, experiments were designed to determine the combined effect of organic and inorganic fertilizers on the growth and development of faba bean. Dabaipi (a cultivar of Vicia faba L.) was used for the experiments. Five treatments with different ratios of organic nitrogen (N) to total N were applied, including 0% organic fertilizer (0% OF), 25% OF, 50% OF, 75% OF, 100% OF and 0% OF. Chemical urea was used as an inorganic fertilizer. The experimental results showed that 50% OF resulted in the highest faba bean yield, up to 10,337.39 and 13,595.7 kg ha−1 in 2018 and 2019, respectively. Compared with 0% OF, 50% OF increased the yield by 84.47% and 183.17%, respectively. The regression analysis showed that the yield could be maximized when ROT accounted for 51.1%. The 50% OF treatment significantly increased N accumulation in seeds, resulting in higher N partial factor productivity and N harvest index (NHI). N accumulation in green seeds and aboveground plant parts had a significantly positive linear correlation with the yield and NHI, respectively. The 50% OF treatment maintained appropriate N accumulation in vegetative organs and higher N accumulation in reproductive organs and whole plants. Compared to 0% OF, the 50% OF treatment increased the total nodule number per plant (52.5%), fresh nodule weight (55.8%), nitrate reductase activity (70.7%), glutamine synthetase (18.2%) and glutamate synthase activity (42.4%). Therefore, the combined application of 50% OF and 50% inorganic fertilizer can be recommended for faba bean cultivation. This study will provide a theoretical basis for high-yield cultivation of faba bean.
    Type of Medium: Online Resource
    ISSN: 2077-0472
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2651678-0
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  • 7
    In: Nature Biomedical Engineering, Springer Science and Business Media LLC, Vol. 6, No. 4 ( 2021-11-22), p. 421-434
    Type of Medium: Online Resource
    ISSN: 2157-846X
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2878897-7
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  • 8
    In: Circulation Research, Ovid Technologies (Wolters Kluwer Health), Vol. 131, No. 1 ( 2022-06-24), p. 6-20
    Abstract: The sino atrial node (SAN) is characterized by the microenvironment of pacemaker cardiomyocytes (PCs) encased with fibroblasts. An altered microenvironment leads to rhythm failure. Operable cell or tissue models are either generally lacking or difficult to handle. The biological process behind the milieu of SANs to evoke pacemaker rhythm is unknown. We explored how fibroblasts interact with PCs and regulate metabolic reprogramming and rhythmic activity in the SAN. Methods: Tbx18 (T-box transcription factor 18)-induced PCs and fibroblasts were used for cocultures and engineered tissues, which were used as the in vitro models to explore how fibroblasts regulate the functional integrity of SANs. RNA-sequencing, metabolomics, and cellular and molecular techniques were applied to characterize the molecular signals underlying metabolic reprogramming and identify its critical regulators. These pathways were further validated in vivo in rodents and induced human pluripotent stem cell-derived cardiomyocytes. Results: We observed that rhythmicity in Tbx18-induced PCs was regulated by aerobic glycolysis. Fibroblasts critically activated metabolic reprogramming and aerobic glycolysis within PCs, and, therefore, regulated pacemaker activity in PCs. The metabolic reprogramming was attributed to the exclusive induction of Aldoc (aldolase c) within PCs after fibroblast-PC integration. Fibroblasts activated the integrin-dependent mitogen-activated protein kinase-E2F1 signal through cell-cell contact and turned on Aldoc expression in PCs. Interruption of fibroblast-PC interaction or Aldoc knockdown nullified electrical activity. Engineered Tbx18-PC tissue sheets were generated to recapitulate the microenvironment within SANs. Aldoc-driven rhythmic machinery could be replicated within tissue sheets. Similar machinery was faithfully validated in de novo PCs of adult mice and rats, and in human PCs derived from induced pluripotent stem cells. Conclusions: Fibroblasts drive Aldoc-mediated metabolic reprogramming and rhythmic regulation in SANs. This work details the cellular machinery behind the complex milieu of vertebrate SANs and opens a new direction for future therapy.
    Type of Medium: Online Resource
    ISSN: 0009-7330 , 1524-4571
    RVK:
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2022
    detail.hit.zdb_id: 1467838-X
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  • 9
    Online Resource
    Online Resource
    Elsevier BV ; 2023
    In:  Chemical Engineering Journal Vol. 465 ( 2023-06), p. 142903-
    In: Chemical Engineering Journal, Elsevier BV, Vol. 465 ( 2023-06), p. 142903-
    Type of Medium: Online Resource
    ISSN: 1385-8947
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 241367-X
    detail.hit.zdb_id: 2012137-4
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  • 10
    In: Agronomy, MDPI AG, Vol. 12, No. 11 ( 2022-10-30), p. 2693-
    Abstract: Fertilizer application, especially their physical and chemical composition, substantially regulates crop growth and development. The form in which fertilizers are applied to the soil has always been regarded as a crucial factor regulating nutrient availability. However, the properties and release characteristics of Mg fertilizers, i.e., fast-release Mg (F-Mg) and slow-release Mg (S-Mg), have not been fully elucidated in acidic soils. This study characterized the different Mg fertilizers, and their release characteristics were verified through pot (using soybean) and field (using pomelo) experiments. The results showed that, despite the differences between different Mg fertilizers, the same functional group peaks were recorded among them. F-Mg fertilizers had a low pH and low Mg purity, while S-Mg fertilizers had a high pH and high Mg purity. The release rate and leaching characteristics of the F-Mg fertilizers were higher than the S-Mg fertilizers. The pot experiment showed that the yield and growth of soybean were higher under the S-Mg fertilizer than the F-Mg fertilizer. However, MgSO4·7H2O and MgO had the best effect among the F-Mg and S-Mg fertilizers, respectively. The effects of these two fertilizers were further validated using field experiments, and it was found that MgSO4·7H2O and MgO fertilizers substantially improved the yield and quality of pomelo. However, MgO showed a better effect than MgSO4·7H2O. This study can provide a sound theoretical basis for selecting the most efficient type of Mg fertilizer for acid soils. It can contribute valuable information regarding farmland management strategies and may result in sustainable agricultural productivity.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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