In:
Journal of Gastroenterology and Hepatology, Wiley, Vol. 21, No. 4 ( 2006-04), p. 647-656
Abstract:
Background and Aim: Intestinal microflora play a crucial role in some severe liver diseases. The purpose of this study was to evaluate the effects of a Lactobacillus strain and a Bifidobacterium strain on ischemia‐reperfusion (I/R) liver injury. Methods: Rats were divided into six groups. Each group received either Bifidobacterium Catenulatum ZYB0401; Lactobacillus Fermentum ZYL0401; a mixture of these two bacterial strains; gentamicin; or saline by daily gavage for 7 days. On the sixth day, all rats, except those in the control group, were subjected to 20 min of liver ischemia. After 22 h of hepatic reperfusion, liver enzymes and histology, malondialdehyde (MDA), superoxide dismutase (SOD), endotoxemia, serum tumor necrosis factor‐α (TNF‐α), intestinal bacteria, intestinal mucosal ultrastructure, and bacterial translocation were studied. Results: All administered bacteria increased intestinal Bifidobacterium and Lactobacillus , decreased endotoxemia ( P 〈 0.01), alanine aminotransferase (ALT) ( P 〈 0.01), and markedly ameliorated liver histology and intestinal mucosal ultrastructure. Only rats treated with Bifidobacterium Catenulatum ZYB0401 and Lactobacillus Fermentum ZYL0401 showed reduced incidence of bacterial translocation to the kidney ( P 〈 0.05), associated with decreased serum TNF‐α and liver MDA ( P 〈 0.05) and increased liver SOD ( P 〈 0.05) compared to the I/R group. Gentamicin decreased almost all kinds of intestinal bacteria ( P 〈 0.01) and decreased ALT ( P 〈 0.01) and serum TNF‐α, but failed to reduce both endotoxemia and the incidence of bacterial translocation and had no effects on liver MDA and SOD. Conclusion: Bifidobacterium Catenulatum ZYB0401 in combination with Lactobacillus Fermentum ZYL0401 could be useful in restoring intestinal microflora and in preventing liver injury in hepatic I/R of rats.
Type of Medium:
Online Resource
ISSN:
0815-9319
,
1440-1746
DOI:
10.1111/jgh.2006.21.issue-4
DOI:
10.1111/j.1440-1746.2006.04306.x
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2006782-3
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