In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 8 ( 2021-8-3), p. e0254730-
Abstract:
Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit’s microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities’ role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0254730
DOI:
10.1371/journal.pone.0254730.g001
DOI:
10.1371/journal.pone.0254730.g002
DOI:
10.1371/journal.pone.0254730.g003
DOI:
10.1371/journal.pone.0254730.g004
DOI:
10.1371/journal.pone.0254730.g005
DOI:
10.1371/journal.pone.0254730.g006
DOI:
10.1371/journal.pone.0254730.g007
DOI:
10.1371/journal.pone.0254730.t001
DOI:
10.1371/journal.pone.0254730.s001
DOI:
10.1371/journal.pone.0254730.s002
DOI:
10.1371/journal.pone.0254730.s003
DOI:
10.1371/journal.pone.0254730.s004
DOI:
10.1371/journal.pone.0254730.s005
DOI:
10.1371/journal.pone.0254730.s006
DOI:
10.1371/journal.pone.0254730.s007
DOI:
10.1371/journal.pone.0254730.s008
DOI:
10.1371/journal.pone.0254730.s009
DOI:
10.1371/journal.pone.0254730.s010
DOI:
10.1371/journal.pone.0254730.r001
DOI:
10.1371/journal.pone.0254730.r002
DOI:
10.1371/journal.pone.0254730.r003
DOI:
10.1371/journal.pone.0254730.r004
DOI:
10.1371/journal.pone.0254730.r005
DOI:
10.1371/journal.pone.0254730.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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