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  • 1
    In: Applied Biochemistry and Biotechnology, Springer Science and Business Media LLC, Vol. 26, No. 1 ( 1990-10), p. 107-113
    Materialart: Online-Ressource
    ISSN: 0273-2289 , 1559-0291
    Sprache: Englisch
    Verlag: Springer Science and Business Media LLC
    Publikationsdatum: 1990
    ZDB Id: 2072711-2
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    Proceedings of the National Academy of Sciences ; 1984
    In:  Proceedings of the National Academy of Sciences Vol. 81, No. 21 ( 1984-11), p. 6647-6651
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 81, No. 21 ( 1984-11), p. 6647-6651
    Kurzfassung: Upon a shift to high temperature, Escherichia coli increase their rate of protein degradation and also the expression of a set of "heat shock" genes. Nonsense mutants of htpR (also called hin), suppressed by a temperature-sensitive suppressor, show lower expression of heat shock genes at 30 degrees C and fail to respond to a shift to 42 degrees C. These mutants were found to have a lower capacity to degrade abnormal or incomplete proteins than that of wild-type cells. This reduction in proteolysis equals or exceeds that in lon mutants, which encode a defective ATP-dependent protease, protease La, and is particularly large in htpR lon double mutants. The activity of protease La was higher in wild-type cells than in htpR mutants grown at 30 degrees C and increased upon shift to 42 degrees C only in the wild type. To determine whether htpR influences transcription of the lon gene, a lon-lacZ operon fusion was utilized. Introduction of the htpR mutation reduced transcription from the lon promoter at 30 degrees C and 37 degrees C. This defect was corrected by a plasmid (pFN97) carrying the wild-type htpR allele. Induction of the heat shock response with ethanol had little or no effect in htpR mutants but stimulated lon transcription 2-3 fold in wild-type cells and htpR cells carrying pFN97. Thus, lon appears to be a heat shock gene, and increased synthesis of protease La under stressful conditions may help to prevent the accumulation of damaged cellular protein.
    Materialart: Online-Ressource
    ISSN: 0027-8424 , 1091-6490
    RVK:
    RVK:
    Sprache: Englisch
    Verlag: Proceedings of the National Academy of Sciences
    Publikationsdatum: 1984
    ZDB Id: 209104-5
    ZDB Id: 1461794-8
    SSG: 11
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Informa UK Limited ; 1988
    In:  Molecular and Cellular Biology Vol. 8, No. 10 ( 1988-10), p. 4295-4301
    In: Molecular and Cellular Biology, Informa UK Limited, Vol. 8, No. 10 ( 1988-10), p. 4295-4301
    Kurzfassung: Covalent attachment of myristic acid to pp60v-src, the transforming protein of Rous sarcoma virus, was studied in a cell-free system. Using a synthetic peptide containing the first 11 amino acids of the mature pp60v-src polypeptide sequence as a substrate, we probed lysates from a variety of cells and tissues for N-myristyl transferase (NMT) activity. Nearly every eucaryotic cell type tested contained NMT, including avian, mammalian, insect, and plant cells. Since NMT activity was detected in rabbit reticulocyte lysates, we took advantage of the translational capability of these lysates to determine the precise point during translation at which myristate is attached to pp60v-src. src mRNA, transcribed from cloned v-src DNA, was translated in reticulocyte lysates which had been depleted of endogenous myristate. Addition of [3H]myristate to lysates 10 min after the start of synchronized translation resulted in a dramatic decrease in the incorporation of radiolabeled myristate into pp60v-src polypeptide chains. These results imply that although myristate can be attached posttranslationally to synthetic peptide substrates, myristylation in vivo is apparently a very early cotranslational event which occurs before the first 100 amino acids of the nascent polypeptide chain are polymerized.
    Materialart: Online-Ressource
    ISSN: 0270-7306 , 1098-5549
    RVK:
    Sprache: Englisch
    Verlag: Informa UK Limited
    Publikationsdatum: 1988
    ZDB Id: 1474919-1
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Online-Ressource
    Online-Ressource
    American Society for Microbiology ; 1984
    In:  Journal of Bacteriology Vol. 158, No. 1 ( 1984-04), p. 180-186
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 158, No. 1 ( 1984-04), p. 180-186
    Kurzfassung: Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viologen, an artificial electron acceptor. A subset of these mutants was studied in which beta-galactosidase was expressed at much higher levels under anaerobic conditions than under aerobic conditions. If nitrate was present during anaerobic growth, the same levels of beta-galactosidase were seen in these fusion strains as were seen under aerobic conditions. The Mu d1(Ap lac) insertions in these mutants were genetically mapped between mutS and srl and thus define a new locus we have termed ant (anaerobic electron transport). Recombinant lambda derivatives were isolated which complemented the deficiency of the ant mutants in anaerobic electron transport and also carried a trans-acting region of DNA which reduced expression of the ant-lac fusions under anaerobic conditions; a probe to the ant region was generated from one of these recombinant lambda derivatives. Southern hybridization analysis revealed that the four independent ant::Mu d1(Ap lac) fusions we isolated spanned an approximately 5-kilobase region and that all were transcribed in the same direction, counterclockwise on the E. coli genetic map.
    Materialart: Online-Ressource
    ISSN: 0021-9193 , 1098-5530
    Sprache: Englisch
    Verlag: American Society for Microbiology
    Publikationsdatum: 1984
    ZDB Id: 1481988-0
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    The American Association of Immunologists ; 1995
    In:  The Journal of Immunology Vol. 155, No. 12 ( 1995-12-15), p. 5647-5654
    In: The Journal of Immunology, The American Association of Immunologists, Vol. 155, No. 12 ( 1995-12-15), p. 5647-5654
    Kurzfassung: Most data available from in vivo sources regarding the impact of somatic hypermutation on Ab V region structure and function are heavily biased due to the influence of clonal selection. In an effort to address this issue directly, we "randomly" introduced point mutations throughout the length of the VH region of an anti-p-azophenylarsonate (Ars) Ab expressed as an Fab in the phage display format. This was accomplished by means of an error-prone PCR with two protocols, which resulted in two mutant libraries. The nature of the nucleotide substitutions obtained from each protocol differed from each other and resulted in different frequencies of phage clones that did not appear to contain Fab on their surfaces. However, the majority of mutants in both libraries lacked detectable Fab expression. Screening of the library containing the most expressed Fabs for those that had gained affinity for structurally related haptens yielded two independent mutants that lacked detectable affinity for Ars and had high affinity for p-azophenylsulfonate. These mutants both contained amino acid substitutions from Asn to Ser or Thr at VH CDR1 position 35, a putative Ars contact residue. In this paper, we discuss the significance of these data with regard to the frequencies of V region loss of function, gain of increased affinity, and gain of altered specificity that result from somatic hypermutation in vivo.
    Materialart: Online-Ressource
    ISSN: 0022-1767 , 1550-6606
    RVK:
    RVK:
    Sprache: Englisch
    Verlag: The American Association of Immunologists
    Publikationsdatum: 1995
    ZDB Id: 1475085-5
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    JSTOR ; 1990
    In:  The Geographical Journal Vol. 156, No. 1 ( 1990-03), p. 96-
    In: The Geographical Journal, JSTOR, Vol. 156, No. 1 ( 1990-03), p. 96-
    Materialart: Online-Ressource
    ISSN: 0016-7398
    Sprache: Unbekannt
    Verlag: JSTOR
    Publikationsdatum: 1990
    ZDB Id: 3040-5
    ZDB Id: 2038485-3
    SSG: 14
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Online-Ressource
    Online-Ressource
    Rockefeller University Press ; 1995
    In:  The Journal of experimental medicine Vol. 182, No. 3 ( 1995-09-01), p. 743-750
    In: The Journal of experimental medicine, Rockefeller University Press, Vol. 182, No. 3 ( 1995-09-01), p. 743-750
    Kurzfassung: To gain insight into the mechanism and limitations of antibody affinity maturation leading to memory B cell formation, we generated a phage display library of random mutants at heavy chain variable (V) complementarity determining region 2 positions 58 and 59 of an anti-p-azophenylarsonate (Ars) Fab. Single amino acid substitutions at these positions resulting from somatic hypermutation are recurrent products of affinity maturation in vivo. Most of the ex vivo mutants retained specificity for Ars. Among the many mutants displaying high Ars-binding activity, only one contained a position 58 and 59 amino acid combination that has been previously observed among the monoclonal antibodies (mAbs) derived from Ars-immunized mice. Affinity measurements on 14 of the ex vivo mutants with high Ars-binding activity showed that 11 had higher intrinsic affinities for Ars that the wild-type V region. However, nine of these Fabs also bound strongly to denatured DNA, a property neither displayed by the wild-type V region nor observed among the mutants characteristic of in vivo affinity maturation. These data suggest that ex vivo enhancement of mAb affinity via site-directed and random mutagenesis approaches may often lead to a reduction in antibody specificity that could complicate the use of the resulting mAbs for diagnostic and therapeutic applications. Moreover, the data are compatible with a hypothesis proposing that increased specificity for antigen, rather than affinity per se, is the driving force for formation of the memory B cell compartment.
    Materialart: Online-Ressource
    ISSN: 0022-1007 , 1540-9538
    RVK:
    Sprache: Englisch
    Verlag: Rockefeller University Press
    Publikationsdatum: 1995
    ZDB Id: 1477240-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    In: Nature Physics, Springer Science and Business Media LLC, Vol. 18, No. 7 ( 2022-07), p. 776-782
    Materialart: Online-Ressource
    ISSN: 1745-2473 , 1745-2481
    Sprache: Englisch
    Verlag: Springer Science and Business Media LLC
    Publikationsdatum: 2022
    ZDB Id: 2206346-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    In: Nuclear Fusion, IOP Publishing, Vol. 59, No. 11 ( 2019-11-01), p. 112021-
    Kurzfassung: For the past several years, the JET scientific programme (Pamela et al 2007 Fusion Eng. Des . 82 590) has been engaged in a multi-campaign effort, including experiments in D, H and T, leading up to 2020 and the first experiments with 50%/50% D–T mixtures since 1997 and the first ever D–T plasmas with the ITER mix of plasma-facing component materials. For this purpose, a concerted physics and technology programme was launched with a view to prepare the D–T campaign (DTE2). This paper addresses the key elements developed by the JET programme directly contributing to the D–T preparation. This intense preparation includes the review of the physics basis for the D–T operational scenarios, including the fusion power predictions through first principle and integrated modelling, and the impact of isotopes in the operation and physics of D–T plasmas (thermal and particle transport, high confinement mode (H-mode) access, Be and W erosion, fuel recovery, etc). This effort also requires improving several aspects of plasma operation for DTE2, such as real time control schemes, heat load control, disruption avoidance and a mitigation system (including the installation of a new shattered pellet injector), novel ion cyclotron resonance heating schemes (such as the three-ions scheme), new diagnostics (neutron camera and spectrometer, active Alfvèn eigenmode antennas, neutral gauges, radiation hard imaging systems…) and the calibration of the JET neutron diagnostics at 14 MeV for accurate fusion power measurement. The active preparation of JET for the 2020 D–T campaign provides an incomparable source of information and a basis for the future D–T operation of ITER, and it is also foreseen that a large number of key physics issues will be addressed in support of burning plasmas.
    Materialart: Online-Ressource
    ISSN: 0029-5515 , 1741-4326
    Sprache: Unbekannt
    Verlag: IOP Publishing
    Publikationsdatum: 2019
    ZDB Id: 2037980-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
    In: Physical Review Research, American Physical Society (APS), Vol. 2, No. 1 ( 2020-1-8)
    Materialart: Online-Ressource
    ISSN: 2643-1564
    Sprache: Englisch
    Verlag: American Physical Society (APS)
    Publikationsdatum: 2020
    ZDB Id: 3004165-X
    Standort Signatur Einschränkungen Verfügbarkeit
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