In:
Chirality, Wiley, Vol. 14, No. 9 ( 2002-01), p. 731-735
Abstract:
The development and validation of a direct injection high‐performance liquid chromatographic (HPLC) method, with column switching, for the determination of metyrapol enantiomers and metyrapone in human plasma is described. The system used in this work was composed of a restricted access media (RAM) bovine serum albumin (BSA) octyl column coupled to an amylose tris(3,5‐dimethoxyphenylcarbamate) chiral column. Water was used as eluent for the first 5 min at a flow rate of 1.0 ml/min for the elution of the plasma proteins and then acetonitrile‐water (30:70 v/v) for the transfer and analysis of metyrapol enantiomers and metyrapone, which were detected by UV at λ = 260 nm. The total analysis time was about 32 min. The calibration curves for each enantiomer and for the metyrapone were linear in the ranges 0.075–0.75 μg/ml and 0.150–1.50 μg/ml, respectively. Recoveries, intra‐ and interday precision and accuracy were determined using three quality controls, one low (0.18 μg/ml), one medium (0.75 μg/ml), and one high (1.35 μg/ml) plasma concentration. Quantitative recoveries and good precision and accuracy were obtained. The limit of quantitation were 0.045 μg/ml for both enantiomers and for the metyrapone. Chirality 14:731–735, 2002. © 2002 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0899-0042
,
1520-636X
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
2001237-8
SSG:
12
SSG:
15,3
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