GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Material
Publisher
Person/Organisation
Language
Years
FID
Subjects(RVK)
  • 1
    Online Resource
    Online Resource
    STRUCTURAL CHARACTERISTICS AND BIOLOGICAL ACTIVITY OF SULFATED POLYSACCHARIDE FROM RED ALGAE BETAPHYCUS GELATINUS
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Science and Technology Vol. 58, No. 6A ( 2021-03-31), p. 252-
    Details
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 58, No. 6A ( 2021-03-31), p. 252-
    Abstract: In this paper, sulfated polysaccharides were extracted from red algae Betaphycus gelatinus species collected at Ninh Thuan sea by using several extraction methods. The structural characteristics of sulfated polysaccharides was investigated by IR and NMR methods. The results indicated that polysaccharide from Betaphycus gelatinus identified as hybrid carrageenan, mainly composed of β-carrageenan, κ-carrageenan and g-carrageenan. Biological activity evaluation showed that the sulfated polysaccharides possessed antioxidant activity with different levels.
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    URL: Article
    DOI: 10.15625/2525-2518/58/6A/15598
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Table_1.DOCX
    Frontiers Media SA ; 2022
    In:  Frontiers in Plant Science Vol. 13 ( 2022-2-2)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-2-2)
    Abstract: Fucoidanases are endo-fucoidanases (also known as endo-fucanases) that catalyze hydrolysis of α-glycosidic linkages in fucoidans, a family of sulfated fucose-rich polysaccharides primarily found in the cell walls of brown seaweeds. Fucoidanases are promising tools for producing bioactive fucoidan oligosaccharides for a range of biomedical applications. High sulfation degree has been linked to high bioactivity of fucoidans. In this study, a novel fucoidanase, Fhf2, was identified in the genome of the aerobic, Gram-negative marine bacterium Formosa haliotis . Fhf2 was found to share sequence similarity to known endo-α(1,4)-fucoidanases (EC 3.2.1.212) from glycoside hydrolase family 107. A C-terminal deletion mutant Fhf2∆484, devoid of 484 amino acids at the C-terminus, with a molecular weight of approximately 46 kDa, was constructed and found to be more stable than the full-length Fhf2 protein. Fhf2∆484 showed endo-fucoidanase activity on fucoidans from different seaweed species including Fucus evanescens , Fucus vesiculosus , Sargassum mcclurei , and Sargassum polycystum . The highest activity was observed on fucoidan from F. evanescens . The Fhf2∆484 enzyme was active at 20–45°C and at pH 6–9 and had optimal activity at 37°C and pH 8. Additionally, Fhf2∆484 was found to be calcium-dependent. NMR analysis showed that Fhf2∆484 catalyzed hydrolysis of α(1,4) linkages between L-fucosyl moieties sulfated on C2 (similar to Fhf1 from Formosa haliotis ), but Fhf2∆484 in addition released oligosaccharides containing a substantial amount of 2,4-disulfated fucose residues. The data thus suggest that the Fhf2∆484 enzyme could be a valuable candidate for producing highly sulfated oligosaccharides applicable for fucoidan bioactivity investigations.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2022.823668
    DOI: 10.3389/fpls.2022.823668.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2687947-5
    detail.hit.zdb_id: 2613694-6
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima
    MDPI AG ; 2020
    In:  Marine Drugs Vol. 18, No. 6 ( 2020-06-02), p. 296-
    Details
    In: Marine Drugs, MDPI AG, Vol. 18, No. 6 ( 2020-06-02), p. 296-
    Abstract: Fucoidans from brown macroalgae (brown seaweeds) have different structures and many interesting bioactivities. Fucoidans are classically extracted from brown seaweeds by hot acidic extraction. Here, we report a new targeted enzyme-assisted methodology for fucoidan extraction from brown seaweeds. This enzyme-assisted extraction protocol involves a one-step combined use of a commercial cellulase preparation (Cellic®CTec2) and an alginate lyase from Sphingomonas sp. (SALy), reaction at pH 6.0, 40 °C, removal of non-fucoidan polysaccharides by Ca2+ precipitation, and ethanol-precipitation of crude fucoidan. The workability of this method is demonstrated for fucoidan extraction from Fucus distichus subsp. evanescens (basionym Fucus evanescens) and Saccharina latissima as compared with mild acidic extraction. The crude fucoidans resulting directly from the enzyme-assisted method contained considerable amounts of low molecular weight alginate, but this residual alginate was effectively removed by an additional ion-exchange chromatographic step to yield pure fucoidans (as confirmed by 1H NMR). The fucoidan yields that were obtained by the enzymatic method were comparable to the chemically extracted yields for both F. evanescens and S. latissima, but the molecular sizes of the fucoidans were significantly larger with enzyme-assisted extraction. The molecular weight distribution of the fucoidan fractions was 400 to 800 kDa for F. evanescens and 300 to 800 kDa for S. latissima, whereas the molecular weights of the corresponding chemically extracted fucoidans from these seaweeds were 10–100 kDa and 50–100 kDa, respectively. Enzyme-assisted extraction represents a new gentle strategy for fucoidan extraction and it provides new opportunities for obtaining high yields of native fucoidan structures from brown macroalgae.
    Type of Medium: Online Resource
    ISSN: 1660-3397
    URL: Article
    DOI: 10.3390/md18060296
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2175190-0
    SSG: 15,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure
    Springer Science and Business Media LLC ; 2021
    In:  Scientific Reports Vol. 11, No. 1 ( 2021-09-30)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2021-09-30)
    Abstract: Fucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). Fucoidans are known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we report the first functional characterization and the first crystal structure of a prokaryotic sulfatase, PsFucS1, belonging to sulfatase subfamily S1_13, able to release sulfate from fucoidan oligosaccharides. PsFucS1 was identified in the genome of a Pseudoalteromonas sp. isolated from sea cucumber gut. PsFucS1 (57 kDa) is Ca 2+ dependent and has an unusually high optimal temperature (68 °C) and thermostability. Further, the PsFucS1 displays a unique quaternary hexameric structure comprising a tight trimeric dimer complex. The structural data imply that this hexamer formation results from an uncommon interaction of each PsFucS1 monomer that is oriented perpendicular to the common dimer interface (~ 1500 Å 2 ) that can be found in analogous sulfatases. The uncommon interaction involves interfacing (1246 Å 2 ) through a bundle of α-helices in the N-terminal domain to form a trimeric ring structure. The high thermostability may be related to this unusual quaternary hexameric structure formation that is suggested to represent a novel protein thermostabilization mechanism.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-021-98588-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2615211-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    The Endo-α(1,3)-Fucoidanase Mef2 Releases Uniquely Branched Oligosaccharides from Saccharina latissima Fucoidans
    MDPI AG ; 2022
    In:  Marine Drugs Vol. 20, No. 5 ( 2022-04-29), p. 305-
    Details
    In: Marine Drugs, MDPI AG, Vol. 20, No. 5 ( 2022-04-29), p. 305-
    Abstract: Fucoidans are complex bioactive sulfated fucosyl-polysaccharides primarily found in brown macroalgae. Endo-fucoidanases catalyze the specific hydrolysis of α-L-fucosyl linkages in fucoidans and can be utilized to tailor-make fucoidan oligosaccharides and elucidate new structural details of fucoidans. In this study, an endo-α(1,3)-fucoidanase encoding gene, Mef2, from the marine bacterium Muricauda eckloniae, was cloned, and the Mef2 protein was functionally characterized. Based on the primary sequence, Mef2 was suggested to belong to the glycosyl hydrolase family 107 (GH107) in the Carbohydrate Active enZyme database (CAZy). The Mef2 fucoidanase showed maximal activity at pH 8 and 35 °C, although it could tolerate temperatures up to 50 °C. Ca2+ was shown to increase the melting temperature from 38 to 44 °C and was furthermore required for optimal activity of Mef2. The substrate specificity of Mef2 was investigated, and Fourier transform infrared spectroscopy (FTIR) was used to determine the enzymatic activity (Units per μM enzyme: Uf/μM) of Mef2 on two structurally different fucoidans, showing an activity of 1.2 × 10−3 Uf/μM and 3.6 × 10−3 Uf/μM on fucoidans from Fucus evanescens and Saccharina latissima, respectively. Interestingly, Mef2 was identified as the first described fucoidanase active on fucoidans from S. latissima. The fucoidan oligosaccharides released by Mef2 consisted of a backbone of α(1,3)-linked fucosyl residues with unique and novel α(1,4)-linked fucosyl branches, not previously identified in fucoidans from S. latissima.
    Type of Medium: Online Resource
    ISSN: 1660-3397
    URL: Article
    DOI: 10.3390/md20050305
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2175190-0
    SSG: 15,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Structural tailoring of fucoidan backbones for maximizing their benefits: Enzymatic, chemical, and physical strategies
    Springer Science and Business Media LLC ; 2023
    In:  Journal of Applied Phycology Vol. 35, No. 5 ( 2023-10), p. 2445-2462
    Details
    In: Journal of Applied Phycology, Springer Science and Business Media LLC, Vol. 35, No. 5 ( 2023-10), p. 2445-2462
    Type of Medium: Online Resource
    ISSN: 0921-8971 , 1573-5176
    URL: Article
    DOI: 10.1007/s10811-023-03036-6
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 1002324-0
    detail.hit.zdb_id: 1477703-4
    SSG: 12
    SSG: 14
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Functional Characterization of a New GH107 Endo-α-(1,4)-Fucoidanase from the Marine Bacterium Formosa haliotis
    MDPI AG ; 2020
    In:  Marine Drugs Vol. 18, No. 11 ( 2020-11-17), p. 562-
    Details
    In: Marine Drugs, MDPI AG, Vol. 18, No. 11 ( 2020-11-17), p. 562-
    Abstract: Fucoidans from brown macroalgae are sulfated fucose-rich polysaccharides, that have several beneficial biological activities, including anti-inflammatory and anti-tumor effects. Controlled enzymatic depolymerization of the fucoidan backbone can help produce homogeneous, defined fucoidan products for structure-function research and pharmaceutical uses. However, only a few endo-fucoidanases have been described. This article reports the genome-based discovery, recombinant expression in Escherichia coli, stabilization, and functional characterization of a new bacterial endo-α-(1,4)-fucoidanase, Fhf1, from Formosa haliotis. Fhf1 catalyzes the cleavage of α-(1,4)-glycosidic linkages in fucoidans built of alternating α-(1,3)-/α-(1,4)-linked l-fucopyranosyl sulfated at C2. The native Fhf1 is 1120 amino acids long and belongs to glycoside hydrolase (GH) family 107. Deletion of the signal peptide and a 470 amino acid long C-terminal stretch led to the recombinant expression of a robust, minimized enzyme, Fhf1Δ470 (71 kDa). Fhf1Δ470 has optimal activity at pH 8, 37–40 °C, can tolerate up to 500 mM NaCl, and requires the presence of divalent cations, either Ca2+, Mn2+, Zn2+ or Ni2+, for maximal activity. This new enzyme has the potential to serve the need for controlled enzymatic fucoidan depolymerization to produce bioactive sulfated fucoidan oligomers.
    Type of Medium: Online Resource
    ISSN: 1660-3397
    URL: Article
    DOI: 10.3390/md18110562
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2175190-0
    SSG: 15,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Teaching According to the Competency-based at Technical Pedagogical Universities
    Bentham Science Publishers Ltd. ; 2022
    In:  The Open Psychology Journal Vol. 15, No. 1 ( 2022-12-23)
    Details
    In: The Open Psychology Journal, Bentham Science Publishers Ltd., Vol. 15, No. 1 ( 2022-12-23)
    Abstract: Six basic characteristics of competency-based teaching are: (1) teaching is conducted based on performance and individual needs; (2) the process can provide immediate learner feedback; (3) performance competencies are determined based on work analysis; (4) the program contains measurable goals and (6) uses performance standards as a reference for performance evaluation. Objective: This study is conducted to investigate the training quality of Technical Pedagogical University according to competency-based teaching. Methods: This study uses a quantitative approach, a survey of 54 school administrators and 186 lecturers from Technical Pedagogical University and 43 school administrators from Vocational Schools in Vietnam. Results: The main findings of the current study are (i) the training quality at Technical Pedagogical University that school administrators and lecturers from Technical Pedagogical University reported was relatively high; (ii) the levels of satisfaction with task requirements at Technical Pedagogical University reported by school administrators at vocational schools were relatively high. Conclusion: Although the evaluation from school administrators and lecturers from the two institutes is quite high, educators should focus on diversified and effective methods to enhance the quality of teaching in educational settings, especially competency-based teaching in universities.
    Type of Medium: Online Resource
    ISSN: 1874-3501
    URL: Article
    DOI: 10.2174/18743501-v15-e221129-2022-29
    Language: English
    Publisher: Bentham Science Publishers Ltd.
    Publication Date: 2022
    detail.hit.zdb_id: 2434631-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Evaluating model teacher education and training at Vietnam's universities of technology and education
    Asian Educational Journal Publishing Group ; 2023
    In:  Journal of Education and e-Learning Research Vol. 10, No. 2 ( 2023-04-26), p. 307-313
    Details
    In: Journal of Education and e-Learning Research, Asian Educational Journal Publishing Group, Vol. 10, No. 2 ( 2023-04-26), p. 307-313
    Abstract: The traditional educational paradigm has become outdated as a result of changes in both the cultural and socioeconomic setting. A more sustainable and acceptable teacher is needed in education. A quantitative study was conducted. 95 administrators and teachers at the University of Technology and Education participated in this study with the aim of analyzing the current status of the teacher education model at the institution. According to the findings of the study, the model for educator preparation has been put into practice primarily through the processes of planning, organizing and directing activities related to educator preparation as well as inspecting and assessing the quality of education. The outcomes of the study indicate that it is essential to design educational programs that are appropriate for the present context. In particular, the study suggests that one of the most important steps towards achieving success is to incorporate technology into teaching methods. The process of educating teachers with the right degree of expertise and skills should be emphasized by educators and policymakers by developing relationships with other educational institutions and allowing teachers to participate in internships.
    Type of Medium: Online Resource
    ISSN: 2410-9991 , 2518-0169
    URL: Article
    DOI: 10.20448/jeelr.v10i2.4612
    Language: Unknown
    Publisher: Asian Educational Journal Publishing Group
    Publication Date: 2023
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Activities of the practice teaching organization and vocational teaching facilities in collaboration between the vocational school and units employing
    Asian Educational Journal Publishing Group ; 2023
    In:  Journal of Education and e-Learning Research Vol. 10, No. 2 ( 2023-04-12), p. 243-249
    Details
    In: Journal of Education and e-Learning Research, Asian Educational Journal Publishing Group, Vol. 10, No. 2 ( 2023-04-12), p. 243-249
    Abstract: The aim of this research is to evaluate the current level of preparation in the organization of practical training as well as the facilities that are available for practical vocational training. The collaboration in education between businesses and vocational schools is an effective strategy. As a result of the rapid transformation that has taken place in the socioeconomic context for professional skills and practical training among employees, a new educational strategy is required to address these demands in order to meet the needs of the workforce. A quantitative method was applied in this research. There were 570 individuals who were chosen at random. According to the findings, the majority of instructors and technicians possessed regulations for conducting practical teaching activities as well as suitable facilities and equipment for the purpose of vocational training. This study presents a number of suggestions for improving the standard of educational institutions as well as the professional growth opportunities available to teachers and lecturers. In addition, facility management and maintenance as well as optimize the instructional facilities and equipment are required.
    Type of Medium: Online Resource
    ISSN: 2410-9991 , 2518-0169
    URL: Article
    DOI: 10.20448/jeelr.v10i2.4588
    Language: Unknown
    Publisher: Asian Educational Journal Publishing Group
    Publication Date: 2023
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...