In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2019-10-01)
Abstract:
We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubiosis and dysbiosis. The profiles of the vaginal microbiome and metabolome were studied in 79 reproductive-aged women, by means of next generation sequencing and proton based-nuclear magnetic resonance spectroscopy. Lactobacillus genus was profoundly depleted in all the genital infections herein considered, and species-level analysis revealed that healthy vaginal microbiome was dominated by L. crispatus . In the shift from HC to CT, VVC, and BV, L. crispatus was progressively replaced by L. iners . CT infection and VVC, as well as BV condition, were mainly characterised by anaerobe genera, e.g. Gardnerella , Prevotella , Megasphaera , Roseburia and Atopobium . The changes in the bacterial communities occurring during the genital infections resulted in significant alterations in the vaginal metabolites composition, being the decrease of lactate a common marker of all the pathological conditions. In conclusion, according to the taxonomic and metabolomics analysis, we found that each of the four conditions is characterized by a peculiar vaginal microbiome/metabolome fingerprint.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-019-50410-x
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2019
detail.hit.zdb_id:
2615211-3
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