In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 63, No. 5 ( 1998-05-01), p. 620-630
Abstract:
Dendritic cells (DC) have been shown to develop along a myeloid or lymphoid lineage of differentiation propagated from bone marrow or early thymic precursor cells with hematopoietic cytokines. In our study, we have induced growth and differentiation of DC from cord blood CD34+ cells initiated in interleukin-2 (IL-2) alone or in IL-2 + stem cell factor (SCF) + tumor necrosis factor α (TNF-α)-supplemented medium and cultured with IL-2 or IL-2 + SCF for 28–35 days. Dendritic morphology and antigenic phenotype of DC grown with IL-2 were characteristic for DC cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Growth and differentiation of DC was followed by an increase in expression of MHC II and co-stimulating molecules CD80 and CD86. We have also shown the expression of the IL-2 receptor (IL-2R) γ-chain in CD34+ cells after 2–3 days of culture with IL-2 alone. The co-expression of the IL-2R α, β, and γ subunits in both DC cultured with IL-2- or GM-CSF-containing cocktail of cytokines was also shown. The time curve for induction of IL-2R demonstrated low levels of subunit expression at the beginning of culture. The number of CD1a cells coexpressing CD25, CD122, and CDγ increased to about 24–68 and to 78–95% after 21 and 28–35 days, respectively. Development of natural killer cells was shown along with DC. The proportion of CD56+ cells and cytotoxicity increased in a time-dependent manner.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.63.5.620
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1998
detail.hit.zdb_id:
2026833-6
SSG:
12
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