In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 7 ( 2023-7-17), p. e0287890-
Abstract:
The purpose of this study was to evaluate, in vivo , the biocompatibility, biomineralization, collagen maturation and the in vitro antibacterial and cytotoxicity of resinous endodontic sealers containing calcium hydroxide. Forty rats were implanted with polyethylene tubes containing Sealer 26, Sealer Plus, Dia-ProSeal and an empty tube, examined after 7, 15, 30 and 60 days. Antimicrobial activity was evaluated against Enterococcus faecalis by Agar Diffusion Test (ADT) through inhibition zones. For cytotoxicity, undifferentiated pulp cells (OD-21) were cultured and assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, exposed to dilution of serial extracts at 6, 24, 48h. Cytotoxicity was analyzed by two-way ANOVA and Bonferroni correction. Kruskal-Wallis test followed by Dunn test was performed for nonparametric data (p 〈 0.05). MTT assay revealed cell proliferation affected by sealers extract in all periods (p 〈 0.0001), except for Dia-Proseal and Sealer Plus ⅛ dilution. Subcutaneous analysis showed at day 7 th moderate inflammatory infiltration. After 30 days, Sealer 26 still showed moderate inflammatory infiltrate compared to mild inflammation from control and Dia-ProSeal (p = 0.006). At day 60 th , all groups showed similar mild inflammatory infiltrate (p 〉 0.05). Sealer 26 induced more biomineralization than other sealers in all periods. At 7 and 15 days, all sealers had significant percentage of immature collagen fibers. After 60 days Sealer 26 showed more mature fibers compared to other sealers (p 〈 0.001). All sealers had a smaller zone of inhibition than chlorhexidine, but with no significant difference among any group (p 〉 0.05). All sealers showed satisfactory biological responses with in vitro / in vivo biocompatibility and antimicrobial activity against planktonic bacteria. Sealer 26 induced more biomineralization than Sealer Plus and Dia-ProSeal.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0287890
DOI:
10.1371/journal.pone.0287890.g001
DOI:
10.1371/journal.pone.0287890.g002
DOI:
10.1371/journal.pone.0287890.g003
DOI:
10.1371/journal.pone.0287890.g004
DOI:
10.1371/journal.pone.0287890.g005
DOI:
10.1371/journal.pone.0287890.g006
DOI:
10.1371/journal.pone.0287890.t001
DOI:
10.1371/journal.pone.0287890.t002
DOI:
10.1371/journal.pone.0287890.t003
DOI:
10.1371/journal.pone.0287890.t004
DOI:
10.1371/journal.pone.0287890.s001
DOI:
10.1371/journal.pone.0287890.s002
DOI:
10.1371/journal.pone.0287890.s003
DOI:
10.1371/journal.pone.0287890.s004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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