In:
Intervirology, S. Karger AG, Vol. 47, No. 6 ( 2004), p. 321-327
Abstract:
〈 i 〉 Objective: 〈 /i 〉 To compare and contrast the intracellular distribution pattern of the human papillomavirus type 59 (HPV 59) E1^E4 protein in COS cells, human keratinocytes, and naturally infected genital epithelium. 〈 i 〉 Methods: 〈 /i 〉 The HPV 59 E1^E4 protein was expressed in COS cells and NIKS cells (immortalized human keratinocytes). A subset of NIKS cells was induced to differentiate. The intracellular distribution pattern of E1^E4 and the effects of E1^E4 expression on the cytoskeleton network were compared for COS and NIKS cells. Expression of E1^E4 was examined in HPV 59-infected foreskin xenografts grown in athymic mice and in a natural HPV 59-infected genital lesion. 〈 i 〉 Results: 〈 /i 〉 The HPV 59 E1^E4 protein formed dense perinuclear inclusions in COS cells, similar to those reported for the HPV 16 E1^E4 protein. In contrast, the E1^E4 protein was diffusely cytoplasmic in undifferentiated NIKS cells, co-localizing with an intact cytokeratin filament network. The E1^E4 protein was concentrated in the region of the cornified cell envelope (CCE) of differentiated NIKS cells, co-localizing with involucrin, a CCE component. A similar distribution in the region of the CCE was observed for E1^E4 protein in HPV 59-infected human epithelial tissues. 〈 i 〉 Conclusions: 〈 /i 〉 The HPV 59 E1^E4 protein is cytoplasmic and co-localizes with an intact cytokeratin filament network in undifferentiated keratinocytes. The E1^E4 protein is distributed in the region of the CCE and co-localizes with involucrin in differentiated human keratinocytes, consistent with the intracellular distribution pattern observed in HPV 59-infected epithelium.
Type of Medium:
Online Resource
ISSN:
0300-5526
,
1423-0100
Language:
English
Publisher:
S. Karger AG
Publication Date:
2004
detail.hit.zdb_id:
184545-7
detail.hit.zdb_id:
1482863-7
SSG:
12
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