In:
British Journal of Haematology, Wiley, Vol. 121, No. 2 ( 2003-04), p. 287-295
Abstract:
Summary. Fluorescence in situ hybridization (FISH) was used to detect 6q–, 11q–, +12, 13q–, 17p– and translocations involving 14q32 in interphase nuclei from blood and/or bone marrow from 113 patients with B‐cell chronic lymphocytic leukaemia (B‐CLL). A total of 87 patients (77%) had a FISH anomaly: 13q– × 1 was most frequent (64%) followed by 13q– × 2 (28%), +12 (25%), 11q– (15%), 17p– (8%) and 6q– (0%). FISH results for blood and bone marrow cells in 38 patients were similar. Purified CD5 + /CD19 + cells from blood were studied in eight patients and results indicate that in some patients not all B cells have FISH anomalies. We used a defined set of hierarchical FISH risk categories to compare FISH results by stable versus progressive disease, age, sex, Rai stage, CD38 + expression and IgV H mutational status. Significant differences in FISH risk distributions were associated with Rai stage, disease status and CD38 + , but not by age, sex or IgV H mutational status. To look for baseline factors associated with high‐risk disease, multivariate analysis of age, sex, Rai stage, CD38 + and disease status versus FISH risk category was performed. Importantly, only CD38 + was significantly associated with high‐risk FISH categories (+12, 11q– and 17p–) after adjustment for the effects of other variables.
Type of Medium:
Online Resource
ISSN:
0007-1048
,
1365-2141
DOI:
10.1046/j.1365-2141.2003.04265.x
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
1475751-5
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