In:
Plant Biotechnology Journal, Wiley, Vol. 12, No. 9 ( 2014-12), p. 1308-1318
Abstract:
RNA ‐ dependent RNA polymerase 6 ( RDR 6) and suppressor of gene silencing 3 ( SGS 3) act together in post‐transcriptional transgene silencing mediated by small interfering RNA s (si RNA s) and in biogenesis of various endogenous si RNA s including the tasi ARF s, known regulators of auxin responses and plant development. Legumes, the third major crop family worldwide, has been widely improved through transgenic approaches. Here, we isolated rdr6 and sgs3 mutants in the model legume Medicago truncatula . Two sgs3 and one rdr6 alleles led to strong developmental defects and impaired biogenesis of tasi ARF s. In contrast, the rdr6.1 homozygous plants produced sufficient amounts of tasi ARF s to ensure proper development. High throughput sequencing of small RNA s from this specific mutant identified 354 potential Mt RDR 6 substrates, for which si RNA production was significantly reduced in the mutant. Among them, we found a large variety of novel phased loci corresponding to protein‐encoding genes or transposable elements. Interestingly, measurement of GFP expression revealed that post‐transcriptional transgene silencing was reduced in rdr6.1 roots. Hence, this novel mis‐sense mutation, affecting a highly conserved amino acid residue in plant RDR 6s, may be an interesting tool both to analyse endogenous pha‐si RNA functions and to improve transgene expression, at least in legume species.
Type of Medium:
Online Resource
ISSN:
1467-7644
,
1467-7652
DOI:
10.1111/pbi.2014.12.issue-9
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2136367-5
SSG:
12
Permalink