In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-10-20)
Abstract:
The temporal regulation of protein abundance and post-translational modifications is a key feature of cell division. Recently, we analysed gene expression and protein abundance changes during interphase under minimally perturbed conditions (Ly et al., 2014, 2015). Here, we show that by using specific intracellular immunolabelling protocols, FACS separation of interphase and mitotic cells, including mitotic subphases, can be combined with proteomic analysis by mass spectrometry. Using this PRIMMUS (PRoteomic analysis of Intracellular iMMUnolabelled cell Subsets) approach, we now compare protein abundance and phosphorylation changes in interphase and mitotic fractions from asynchronously growing human cells. We identify a set of 115 phosphorylation sites increased during G2, termed ‘early risers’. This set includes phosphorylation of S738 on TPX2, which we show is important for TPX2 function and mitotic progression. Further, we use PRIMMUS to provide the first a proteome-wide analysis of protein abundance remodeling between prophase, prometaphase and anaphase.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.27574.001
DOI:
10.7554/eLife.27574.002
DOI:
10.7554/eLife.27574.003
DOI:
10.7554/eLife.27574.004
DOI:
10.7554/eLife.27574.005
DOI:
10.7554/eLife.27574.006
DOI:
10.7554/eLife.27574.007
DOI:
10.7554/eLife.27574.008
DOI:
10.7554/eLife.27574.009
DOI:
10.7554/eLife.27574.010
DOI:
10.7554/eLife.27574.011
DOI:
10.7554/eLife.27574.012
DOI:
10.7554/eLife.27574.013
DOI:
10.7554/eLife.27574.014
DOI:
10.7554/eLife.27574.015
DOI:
10.7554/eLife.27574.016
DOI:
10.7554/eLife.27574.017
DOI:
10.7554/eLife.27574.018
DOI:
10.7554/eLife.27574.019
DOI:
10.7554/eLife.27574.020
DOI:
10.7554/eLife.27574.021
DOI:
10.7554/eLife.27574.023
DOI:
10.7554/eLife.27574.024
DOI:
10.7554/eLife.27574.025
DOI:
10.7554/eLife.27574.026
DOI:
10.7554/eLife.27574.027
DOI:
10.7554/eLife.27574.029
DOI:
10.7554/eLife.27574.030
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
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